Brucella vaccine and method for preparing antigen protein used for same

Abstract

The invention discloses a brucella vaccine applied to livestock and a method for preparing antigen protein used for same. The antigen protein used for the brucella vaccine is brucella bcsap31 protein, and in particular brucella bcsp31 restructured protein. The method for preparing the restructured protein has steps of: taking bacterium total DNA extracted from the brucella as a template, designing a pair of primers according to ribotide complete sequence of brucella bcsp31 gene to carry out polymerase chain reaction, obtaining bcsp31 protein gene by clone and then transferring the bcsp31 protein gene to a yeast expression vector to obtain a restructured expression vector, importing the obtained restructured expression vector to yeast expression bacterium to obtain restructured pichia pastoris expression bacterium, and carrying out proliferation and induced expression of the restructured pichia pastoris expression bacterium so as to obtain the required antigen protein by separation and purification.

Description

technical field [0001] The invention relates to a vaccine against brucellosis used by domestic animals and a preparation method of the antigenic protein used in the vaccine. Background technique [0002] Livestock brucellosis is distributed worldwide. It exists or breaks out in more than 170 countries and regions among more than 200 countries in five continents, especially in many developing countries in South America, Africa and Asia. Brucellosis is mainly infected through the digestive tract, and generally only occurs between animals-animals and animals-humans. It is listed as a second-class infectious disease by the country. In recent years, the epidemic situation of brucellosis in my country has rebounded obviously, and has spread to 28 provinces, autonomous regions and municipalities directly under the Central Government; the outbreak of livestock brucellosis has caused huge economic losses to the animal husbandry. The epidemic situation of brucellosis is very serious....

AI Technical Summary

Problems solved by technology

However, long-term use of weak strains may become more virulent

In addition, the cultivation of Brucella requires high conditions and is difficult to cultivate.

On the other hand, since the current serological detection technology in my country cannot distinguish vaccine immune antibodies from wild virus infection antibodies

Images