Clean production method for preparing N-(phosphonomethyl)iminodiacetic acid
A technology of clean production and diglyphosate, which is applied in the chemical industry, can solve the problems of no mention of diglyphosate wastewater treatment methods, no good wastewater treatment methods, etc., and achieve the effects of reducing hydrolysis costs, simple composition, and low salt content
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Embodiment 1
[0024] Those skilled in the art know that there are many kinds of enzymes that can make nitrile hydrolysis, and the enzymes used in the following examples here are genetically engineered Escherichia coli BL21 (DE3) / Pet-NiT (LuoHui, et al.Appl Biochem Biotechnol.DOI10.1007 / s12010-008-8324-y).
[0026] Medium:
[0027] 1# medium composition: tryptone 1%, yeast powder 0.5%, sodium chloride 1%, glycerol 0.3%, 20mM lactose, kanamycin 50uM / L, PH7.0
[0028] 2# medium composition: 5% corn steep liquor, 0.017M KH 2 PO 4 , 0.07M K 2 HPO 4 , 0.4% glucose, 20mM lactose, 50uM / L kanamycin, pH7.5
[0029] nourish:
[0030] In medium 1#, 35°C, 180r / min, cultivate genetically engineered Escherichia coli BL21(DE3) / Pet-NiT for 12h, inoculate 5% inoculum into medium 2#, 30°C, 200r / min , cultivated for 14h.
[0031] Collect bacteria:
[0032] 10000r / min, centrifuge for 5min, and harvest the bacteria.
[0033] Immobilized enzyme preparation:
[0034] First...
Embodiment 2
[0039] Nitrilase was immobilized on the carrier obtained by mixing sodium periodate and starch, then added to the evaporated solution in Example 1, and added water to 1450 g. Slowly add iminodiacetonitrile between PH6-9 at 35°C to maintain the concentration of diacetonitrile. After 14 hours of dosing, stop the dosing and add 100 g of iminodiacetonitrile (92%) in total, (during which it is used to adjust the pH Consume liquid caustic soda 27g) filter after insulation 1h, get filtrate 1531.8g, IDA content 8.24%, get the diglyphosate mother liquor 300g among the embodiment 1 and mix with filtrate after adjusting pH to 6-7 with 7g tablet alkali. Then carry out membrane concentration to obtain concentrated solution 790.4g, concentrated solution is evaporated again, take embodiment 1 middle membrane concentrated light solution 300g absorb ammonia gas, obtain concentrated solution 489.3g, IDA content 26.13%. Get the concentrated solution and add 152g (99%) phosphorus trichloride drop...
Embodiment 3
[0041]Take the immobilized enzyme filtered in Example 1 and add some unused immobilized enzyme, dissolve in the aqueous solution of pH=7, a total of 1100g, the enzyme cell concentration is 40g / L. Slowly add iminodiacetonitrile at 40°C, pH 6-9, maintain the concentration of diacetonitrile at 0.05mol / L-0.1mol / L, stop adding after 18 hours of reaction, and add iminodiacetonitrile (92%) 100g, (during this period, 22g of liquid caustic soda is used for adjusting PH) is incubated for 1h and then filtered to obtain 1198.3g of filtrate with an IDA content of 10.51%. After evaporation, 435.3 g of concentrated liquid was obtained, and 263.6 g of evaporated liquid was obtained. Take the concentrated solution and add 155g (99%) phosphorus trichloride dropwise in a 1000ml flask, then raise the temperature and add dropwise 101.9g (37%) formaldehyde, after the reaction is completed, 140g of deacidification, add 62g of liquid caustic soda (32%) to adjust the pH, and filter with suction , 100...
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