Protocatechuic acid-3-O-(6-hydroxybenaene benzoyl)-beta-D-heteroside extracted from isodon lophanthoides and method
A technology of o-hydroxybenzoyl and protocatechuic acid is applied in the field of protocatechuic acid-3-O--β-D-glucoside and its preparation, and achieves the effects of improving drug efficacy, advanced preparation method and easy operation
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Embodiment 1
[0020] Take 6.0kg of the whole herb of fresh thread-shaped fragrant tea from Henan, and use 50% acetone in a flash extractor at room temperature 21°C to extract twice, 15 minutes each time, combine the extracts, and recover the solvent under reduced pressure at 45°C , dry powder was obtained after vacuum drying, the dry powder was dissolved in water, centrifuged, the supernatant was applied to a macroporous adsorption resin Diaion HP-20 column, and the column was washed with water until the eluent flowed out colorless, and the column was washed with 10% methanol until the eluent flowed out Colorless, flush the column with 20% methanol until the eluent flows out colorless, and finally flush the column with 30% methanol until the eluent flows out colorless, collect the 30% methanol eluate, recover the solvent under reduced pressure, and obtain a dry powder after vacuum drying 19.6g, dissolved in water, centrifuged, put the supernatant on the gel Toyopearl HW-40C column, washed th...
Embodiment 2
[0022] Take 8.0kg of the whole herb of fresh scalloped tea from Yunnan or Guangxi, and use 50% acetone to crush and extract twice in a flash extractor at room temperature at 23°C, each time for 18 minutes, combine the extracts, and depressurize at 45°C Recover the solvent, dry it in vacuum to get dry powder, dissolve the dry powder in water, centrifuge, put the supernatant on the macroporous adsorption resin Diaion HP-20 column, wash the column with water until the eluate flows out colorless, and wash the column with 10% methanol until the elution The eluate is colorless, flush the column with 20% methanol until the eluent is colorless, and finally flush the column with 30% methanol until the eluent is colorless, collect the 30% methanol eluate, recover the solvent under reduced pressure, and vacuum dry Obtain 25.8g of dry powder, dissolve it in water, centrifuge, put the gel Toyopearl HW-40C column on the supernatant, wash the column with water, 10% methanol, and 20% methanol ...
Embodiment 3
[0024] Take 10.0kg of the whole herb of fresh line-grained tea leaves produced in Hunan or Zhejiang, and use 50% acetone in a flash extractor at a room temperature of 25°C to crush and extract twice, 20 minutes each time, combine the extracts, and reduce them at 45°C. Recover the solvent under pressure, and obtain a dry powder after vacuum drying. The dry powder is dissolved in water, centrifuged, and the supernatant is placed on a macroporous adsorption resin DiaionHP-20 column. The eluate is colorless, flush the column with 20% methanol until the eluent is colorless, and finally flush the column with 30% methanol until the eluent is colorless, collect the 30% methanol eluate, recover the solvent under reduced pressure, and vacuum dry Obtain 33.0 g of dry powder, dissolve it in water, centrifuge, put the supernatant on the gel Toyopearl HW-40C column, wash the column with water, 10% methanol, and 20% methanol successively to obtain an eluate, and drop the eluate on a thin-laye...
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Abstract
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