Method for in-vitro liver cell differentiation of human marrow mesenchyme stem cells based on VPA inducement

Abstract

The invention discloses a novel method for the in-vitro liver cell differentiation of human marrow mesenchyme stem cells based on VPA inducement. The human marrow is used as a raw material, the mesenchyme stem cells of the human marrow are separated, a fibroblast growth factor 4, a hepatocyte growth factor, tumour suppressing essence and dexamethasone are sequentially added after the VPA processing of histone ab-acetylase suppressor valproic acid, the liver cells of the human marrow are induced to be differentiated, and a class of polygonal liver cell type cells which have the biological functions of the liver cells of synthesis glycogen, urea, secretion albumin and the like and have double cores are obtained. The method enhances the efficiency of the liver cell differentiation of the human marrow mesenchyme stem cells greatly, provides a novel technology and opens up a novel approach for developing and applying the human marrow mesenchyme stem cells to the cellular transplantation treatment of liver diseases, hepatic tissue engineering and the like and also provides a novel research model for the research of liver cell differentiation development molecular mechanisms comprising a surface appearance genetic mechanism and the like.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to the discovery of a histone deacetylase inhibitor valproic acid (valproic acid, VPA) to induce human bone marrow mesenchymal stem cells (humanbone marrow mesenchymal stem cells, hBMMSCs) to span and differentiate liver cells in vitro Technology. Background technique [0002] Stem cells are a type of cells with self-renewal and multi-directional differentiation potential, which can be divided into embryonic stem cells and adult stem cells according to their developmental stages. Adult stem cells widely exist in bone marrow, nerve, muscle, epidermis, intestine, liver and other tissues or organs. The traditional view is that adult stem cells can only differentiate into the tissue types they settle in, but cannot differentiate into cells of other tissue types. However, studies in recent years have shown that adult stem cells can differentiate into cells of other tissue types different from ...

AI Technical Summary

Problems solved by technology

In recent years, researchers have used experimental animal models such as rats and mice to conduct more research on the differentiation of BMMSCs into hepatocytes in vitro, and initially established some differentiation induction techniques, such as using mouse damaged liver tissue conditioned medium and fetal Liver tissue conditioned medium induces mouse BMMSCs to differentiate into hepatocytes in vitro, using different cytokines (such as SCF, HGF, EGF and FGF-4, etc.) It is rare, and most of the research work is focused on the establishment of different cytokine combinations to induce differentiation, and there is no technical system using the histone deacetylase inhibitor VPA to induce the differentiation of hepatocytes

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