Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for in-vitro liver cell differentiation of human marrow mesenchyme stem cells based on VPA inducement

A technology of bone marrow mesenchymal and hepatocytes, applied in the field of biomedicine, can solve the problems of rare research on differentiated hepatocytes, and achieve the effect of improving in vitro differentiation efficiency, increasing differentiation rate, and obvious induction effect

Inactive Publication Date: 2010-05-19
SHAOXING PEOPLES HOSPITAL
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, researchers have used experimental animal models such as rats and mice to conduct more research on the differentiation of BMMSCs into hepatocytes in vitro, and initially established some differentiation induction techniques, such as using mouse damaged liver tissue conditioned medium and fetal Liver tissue conditioned medium induces mouse BMMSCs to differentiate into hepatocytes in vitro, using different cytokines (such as SCF, HGF, EGF and FGF-4, etc.) It is rare, and most of the research work is focused on the establishment of different cytokine combinations to induce differentiation, and there is no technical system using the histone deacetylase inhibitor VPA to induce the differentiation of hepatocytes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for in-vitro liver cell differentiation of human marrow mesenchyme stem cells based on VPA inducement
  • Method for in-vitro liver cell differentiation of human marrow mesenchyme stem cells based on VPA inducement
  • Method for in-vitro liver cell differentiation of human marrow mesenchyme stem cells based on VPA inducement

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Isolation and culture of human bone marrow mesenchymal stem cells

[0041] Firstly, mononuclear cells are isolated from human bone marrow samples, and the bone marrow from adult healthy volunteers is washed once or twice with phosphate buffered saline (PBS), and the bone marrow is diluted into a cell suspension, and gently added to human lymph The upper layer of the cell separation solution (Ficoll-Hypaque, D=1.077±0.002g / ml); after centrifugation at room temperature 2200rpm / min for 25 minutes, the upper layer of PBS was aspirated, and the middle white cell layer (mononuclear cell layer) was taken; the basal culture medium ( Contain 10% fetal bovine serum FBS, 100mg / ml streptomycin 100U / ml penicillin low-sugar IMDM) to wash the cells, then centrifuge at a low speed of 1000rpm / min for 6-7min, pour off the culture medium, and use this culture medium to dilute the cells to a certain concentration Inoculate the suspension in a culture bottle, place it in a 37°C, 5% CO2 incu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a novel method for the in-vitro liver cell differentiation of human marrow mesenchyme stem cells based on VPA inducement. The human marrow is used as a raw material, the mesenchyme stem cells of the human marrow are separated, a fibroblast growth factor 4, a hepatocyte growth factor, tumour suppressing essence and dexamethasone are sequentially added after the VPA processing of histone ab-acetylase suppressor valproic acid, the liver cells of the human marrow are induced to be differentiated, and a class of polygonal liver cell type cells which have the biological functions of the liver cells of synthesis glycogen, urea, secretion albumin and the like and have double cores are obtained. The method enhances the efficiency of the liver cell differentiation of the human marrow mesenchyme stem cells greatly, provides a novel technology and opens up a novel approach for developing and applying the human marrow mesenchyme stem cells to the cellular transplantation treatment of liver diseases, hepatic tissue engineering and the like and also provides a novel research model for the research of liver cell differentiation development molecular mechanisms comprising a surface appearance genetic mechanism and the like.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to the discovery of a histone deacetylase inhibitor valproic acid (valproic acid, VPA) to induce human bone marrow mesenchymal stem cells (humanbone marrow mesenchymal stem cells, hBMMSCs) to span and differentiate liver cells in vitro Technology. Background technique [0002] Stem cells are a type of cells with self-renewal and multi-directional differentiation potential, which can be divided into embryonic stem cells and adult stem cells according to their developmental stages. Adult stem cells widely exist in bone marrow, nerve, muscle, epidermis, intestine, liver and other tissues or organs. The traditional view is that adult stem cells can only differentiate into the tissue types they settle in, but cannot differentiate into cells of other tissue types. However, studies in recent years have shown that adult stem cells can differentiate into cells of other tissue types different from ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
Inventor 董学君邵健忠项黎新张卉杨超
Owner SHAOXING PEOPLES HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com