Japanese encephalitis virus JEV replicon vector and application thereof

A technology of encephalitis virus and replicon, which is applied in the direction of virus/bacteriophage, antiviral agent, virus antigen component, etc., and can solve problems such as potential safety hazards
CN101712965AInactive Publication Date: 2010-05-26INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
Publication Date
2010-05-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a replicon vector taking Japanese encephalitis virus genome as a framework, as well as a cell line for packaging the same and a packaging system. The JEV replicon vector has the capability of efficiently expressing foreign protein. Mice are immunized with the replicon vector, and the titer of an anti-JEV antibody reaches 1:1280 after three immunizations so as to protect 75 percent of suckling mice from virus attack. The cell line provided can produce 1.6*105 U / ml of pseudovirus particles after packaging JEV replicon, and the titer of the anti-JEV antibody reaches 1:2560 after two immunizations so as to protect 73 percent of suckling mice from virus attack. The invention establishes a technical platform for a JEV replicon vector system for the first time, and explores the feasibility of researching replicon vaccines and pseudovirus vaccines through the JEV replicon vector system, thereby laying a solid foundation for developing and researching a plurality of novel vaccines used to prevent and treat tumors and viral diseases in future.
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Description

technical field

[0001] The invention relates to a replicon carrier with Japanese encephalitis virus genome as the backbone, and a cell line and packaging system used for packaging the replicon carrier. More specifically, the present invention relates to a JEV expression system that can encode exogenous immunogenic proteins and polypeptides that can be used as a vaccine delivery system. Vaccines can be administered in the form of DNA or virus-like particles, and the carrier system and its derivative products can be used for gene therapy and gene prevention research. Background technique

[0002] JEV belongs to the genus Flaviviridae and is transmitted by mosquitoes. It is an important human pathogen and can cause permanent neurological diseases and even fatal diseases. There are more than 50,000 cases each year, the fatality rate exceeds 25%, and more than half of the survivors will have lasting neurologic sequelae. JEV is mainly distributed in Asia, from the Soviet Union t...

Claims

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