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Preparation for treating and restoring infective wound surface and preparation method thereof

A preparation and wound technology, applied in the field of medicine, can solve the problems of poor bactericidal effect of bacterial infection wounds, inability to effectively kill pathogenic bacteria, ineffective control, etc., and achieve the effects of accelerating wound healing, suitable for mass production, and reducing the probability of contact.

Active Publication Date: 2013-07-10
SHANGHAI HI TECH BIOENG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can interact with locally applied agents and endogenous molecules in the body to promote wound healing, but it has poor bactericidal effect on bacterially infected wounds and cannot effectively treat such wounds
[0004] The above-mentioned dressings generally have the disadvantages that they cannot effectively kill the pathogenic bacteria that cause wound infection, or cannot effectively control and absorb wound exudate, etc.

Method used

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  • Preparation for treating and restoring infective wound surface and preparation method thereof
  • Preparation for treating and restoring infective wound surface and preparation method thereof
  • Preparation for treating and restoring infective wound surface and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Preparation of chitosan-collagen hydrogel loaded with lysostaphin enzyme

[0032] Weigh 0.1g of chitosan hydrochloride and dissolve it in 10ml of water, add 0.3g of hydrolyzed collagen (molecular weight 3000Da), stir evenly, add 0.12g of water-soluble carbodiimide hydrochloride (EDAC), and stir at room temperature for 2h .

[0033] Add 0.1 g of lysostaphin into the prepared chitosan-collagen hydrogel, and stir evenly to prepare the lysostaphin-loaded chitosan-collagen hydrogel.

Embodiment 2

[0034] Example 2 Preparation of chitosan-collagen hydrogel scaffold loaded with lysostaphin enzyme

[0035] Pour the lysostaphin-loaded chitosan-collagen hydrogel prepared in Example 1 into a petri dish (40×25 mm), and freeze-dry to obtain a hydrogel scaffold.

Embodiment 3

[0036] Embodiment 3: chitosan-collagen hydrogel scaffold scanning electron microscope

[0037] Take chitosan-collagen hydrogel scaffold (the mass ratio of chitosan to collagen is 1:1, 1:3, 1:6 and 1:8, respectively) and lysostaphin-loaded hydrogel scaffold (1:6) Spray gold, observe with scanning electron microscope, and take pictures. The result is as figure 1 and figure 2 shown. It can be seen that there are more pores in the hydrogel scaffold ( figure 1 ), while lysostaphin is uniformly dispersed in the pores ( figure 2 ).

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Abstract

The invention belongs to the technical field of medication, in particular to a biological preparation for treating and restoring an infective wound surface and a preparation method thereof. The preparation disclosed by the invention comprises the following components in percentage by weight: 0.1 to 30 percent of staphylococcus lysozyme, 3 to 50 percent of chitosan or chitosan derivative and 30 to95 percent of collagen. The preparation method comprises the following steps: crosslinking the natural biodegradable chitosan derivative and the collagen serving as dressings to form hydrogel used asa carrier; and loading the staphylococcus lysozyme into the hydrogel to prepare a novel preparation with a function of promoting the healing of the wound surface and a bactericidal effect.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a biological preparation for treating and repairing infected wounds and a preparation method thereof. Background technique [0002] Reconstructing or restoring the skin barrier is the ultimate goal of wound treatment. A wound covering with excellent performance can temporarily play a part of the skin barrier function and provide an environment conducive to wound healing. An ideal wound dressing should first effectively prevent bacterial infection, especially the infection of methicillin-resistant Staphylococcus aureus (MRSA), improve the success rate of skin grafting and accelerate wound healing, and at the same time should have good biocompatibility, control and absorb the wound Exudation, suitable for gas and water vapor permeation, protection of new tissue, acceleration of wound healing and other properties. [0003] The kind of existing dressing is a lot, is rou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/48A61P17/02A61L15/44A61K38/39A61K31/722
Inventor 黄青山崔福英
Owner SHANGHAI HI TECH BIOENG
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