Method for measuring content of lactitol

Abstract

The invention provides a method for measuring the content of lactitol, which adopts a high performance liquid internal standard method to carry out the measuring. The method comprises the following steps: (1) preparing a lactitol sample to be measured, and adding the lactitol sample to arabinose solution, so as to dissolve and dilute the lactitol sample; (2) analyzing the lactitol sample to be measured by HPLC, wherein carbohydrate analytical columns are selected as chromatographic columns, calcium-ion coordination exchanger is adopted as padding, water is adopted as a mobile phase, arabinoseis adopted as the internal standard, the temperature of the columns is 90 DEG C, the flow rate is 0.5mL / min, a differential detector is adopted, the sample injection volume is 20mul, and the theoretical plate number is larger than or equal to 3,000. The invention has the advantages of accurate measuring, convenient operation, high specificity and high sensitivity.

Description

technical field [0001] The invention relates to a chromatographic monitoring method, in particular to a method for determining the content of lactitol by high performance liquid chromatography. Background technique [0002] Lactitol (also known as lactitol or lactitol monohydrate) is a disaccharide derivative composed of sorbitol and galactose, which is not hydrolyzed by disaccharide enzymes in the gastrointestinal tract, but enters the colon in its original form. In the colon, it is degraded by intestinal flora (mainly Bacteroides and Lactobacillus) into short-chain organic acids (mainly acetic acid, propionic acid, and butyric acid), acidifying the colonic contents, thereby reducing the absorption of ammonia by the colon. This product can be converted into low-molecular-weight organic acids, leading to an increase in the osmotic pressure in the colon, thereby increasing the water content and volume of the stool, and producing a laxative effect. Because of its sweet taste,...

AI Technical Summary

Problems solved by technology

In the local standard of Shanghai, it is announced that the oxidation-reduction titration method is used to determine its content, but since the starting material for the preparation of lactitol is lactose, the intermediates mannitol, sorbitol, and galactitol can all participate in the oxidation-reduction reaction. It is easy to interfere with the determination of the content and affect the final test result; and this method also has some shortcomings of the usual chemical method, such as complicated operation, visual error in the judgment of the end point, etc.; therefore, the redox titration method is used to determine the content of lactitol , has the disadvantages of inaccurate test results, method specificity, reproducibility, poor recovery rate, cumbersome operation, visual error, etc.

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