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Peristaltic solid state fermentation reactor for cellulose fermentation volatile alcohol and fermentation method thereof

A solid-state fermentation and reactor technology, applied in specific-purpose bioreactors/fermenters, solid-phase fermentation bioreactors, bioreactor/fermenter combinations, etc., can solve the problems of mass transfer, heat transfer, microbial Cell damage, damage to microbial cells and other problems, to achieve the effect of improving fermentation efficiency, reducing product inhibition, and solving fermentation microbial cell damage

Inactive Publication Date: 2013-03-27
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that the existing cellulose fermentation volatile alcohol reactor cannot solve the problem of mass transfer and heat transfer, or the use of agitation to enhance mass transfer and heat transfer brings about the problem of agitation and shear force damage to microbial cells, and proposes a The peristaltic solid-state fermentation reactor and its fermentation method for cellulose fermentation of volatile alcohols, using up and down moving spheres to imitate the peristalsis of the small intestine, realize the back and forth push of solid materials, enhance the mass transfer and heat transfer effects, and promote the solid materials by the spheres The force belongs to the normal force, which can solve the problem of the damage of the stirring shear force to the microbial cells, and realize the online separation of volatile alcohols by using air stripping, and reduce the product inhibition in the fermentation process

Method used

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  • Peristaltic solid state fermentation reactor for cellulose fermentation volatile alcohol and fermentation method thereof
  • Peristaltic solid state fermentation reactor for cellulose fermentation volatile alcohol and fermentation method thereof

Examples

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Embodiment 1

[0032] A peristaltic solid-state fermentation reactor for cellulose fermentation of volatile alcohol in this embodiment has an aspect ratio of 3, a total design volume of 50 L, and the up and down reciprocating motion of the shaft is realized by a motor combined with a crankshaft and connecting rod.

[0033] The fermentation operation steps are as follows: use crushed steam-exploded corn stalks with a water content of 50% and a cellulose content of 35% as raw materials, use 0.1Mpa high-pressure steam to sterilize, and use a cellulase dosage of 20IU filter paper enzyme activity / g pre-treated cellulose Raw material, cellulase is sterilized by 0.45μm ultrafiltration membrane, 40L of cellulose raw material and cellulase are added to the fermenter, inoculated with Angelica high activity dry yeast Saccharomyces cerevisiae (0.2% inoculum), and added nutrients (0.1% (NH 4 ) 2 SO 4 , 0.1% KH 2 PO 4 ), adjust the pH to 4.8-5.0, control the temperature in the fermentation tank at 35°...

Embodiment 2

[0035] This embodiment adopts the fermentation reactor of Embodiment 1, and its fermentation operation steps are as follows: use the pulverized steam-exploded corn stalks with a water content of 50% and a cellulose content of 35% as raw materials, use 0.1Mpa high-pressure steam to sterilize, and cellulase Amount of 20IU filter paper enzyme activity / g pretreatment cellulosic raw material, cellulase use 0.45 μ m ultrafiltration membrane filtration sterilization, 40L of cellulosic raw material and cellulase are added in the fermenter together, inoculate Clostridium acetobutylicum ( C.acetobutylicum AS1.132), inoculum size 10%, and added nutrients (0.2% (NH 4 ) 2 SO 4 , 0.2% yeast extract, 0.05% KH 2 PO 4 , 0.05%K 2 HPO 4 ), adjust the pH to 6.5-7.0, control the temperature in the fermenter at 37°C, turn on the motor on the shaft, make the shaft drive the ball to move up and down, push the solid material to move back and forth, and use CO 2 Replace the air in the system, and...

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Abstract

The invention discloses a peristaltic solid state fermentation reactor for cellulose fermentation volatile alcohol and a fermentation method thereof; the reactor comprises a bionic peristaltic fermentation reactor (1), peristaltic actuating devices (7, 8), circulating gas stripping devices (13, 14), and heat pump separation devices (9, 10, 11, 12); shafts distributed in a concentric circle way are arranged in the peristaltic fermentation reactor, round balls are distributed on the shaft with equal space, the shaft drives the ball to move up and down, so as to realize back and forth pushing tosolid material; the steps of fermenting volatile alcohol by cellulose are as follows: the cellulose, cellulose enzyme and fermentation organisms are added in a tank body for carry out solid state fermentation, the fermentation products can be taken out by utilizing circulating gas stripping, and the products are condensed and collected at the position of a heat pump evaporator. The invention has the advantages that: the movement that the ball body drives the solid material enhances mass transferring and heat transferring effect, the pushing force of the ball body to the solid material is normal acting force, so as to solve the problem that stirring shearing force damages the microbial cells.

Description

technical field [0001] The invention belongs to the technical field of cellulose fermentation industry, in particular to a peristaltic solid-state fermentation reactor for cellulose fermentation of volatile alcohol and a fermentation method thereof. Background technique [0002] Since the last century, as the world's population has increased and more countries have industrialized, energy consumption has also increased. Many experts estimate that the known oil reserves in the world will be consumed within about 30 years, so countries all over the world have launched research and development of new energy sources. Solar energy is an inexhaustible and inexhaustible renewable energy source for human beings. Plants efficiently use solar energy to synthesize lignocellulose, which is a huge renewable resource. It is estimated that lignin and cellulose, the main components of plant biomass, are continuously regenerated at a rate of about 164 billion tons per year, which is equivale...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/02C12P7/02
CPCC12M21/12C12M21/18C12M21/16C12M29/06C12M29/24C12M27/02Y02P20/129
Inventor 陈洪章丁文勇
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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