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Plant pathogenic fungi fusarium moniliforme natural antagonist

A technology of Fusarium moniliformes and compounds, which can be applied in the directions of plant growth regulators, fungicides, fungi, etc., can solve the problems that do not involve Fusarium nidulans, etc.

Inactive Publication Date: 2010-07-14
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the domestic and foreign patent literature reports, there is no report on the compound chlorodiscosyrin A itself and its ability to inhibit the activity of Fusarium moniliform (F.moniliform)

Method used

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  • Plant pathogenic fungi fusarium moniliforme natural antagonist
  • Plant pathogenic fungi fusarium moniliforme natural antagonist
  • Plant pathogenic fungi fusarium moniliforme natural antagonist

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The separation of embodiment 1 Pseudomonas fumigatus (P.adusta) SN17

[0018] P.adusta SN17, which was isolated from Hainan Province, was deposited in the General Microorganism Center (CGMCC) of China Committee for Culture Collection of Microorganisms on September 26, 2008 (大学, Chaoyang District, Beijing, China). Tunlu Institute of Microbiology, Chinese Academy of Sciences, 100101), the preservation number is 2672.

[0019] The separation method adopts the conventional endophytic fungus separation method, and the specific steps are as follows: wash the healthy plant branches and leaves with water, dry them, soak them in 75% alcohol for disinfection for 60 seconds, soak them in 25% (1.3% available chlorine) sodium hypochlorite Disinfect for 5 minutes, then soak and disinfect with 75% alcohol for 30 seconds, then wash with sterile water for 3 times, cut the sterilized tissue into small pieces with a side length of about 5mm, place them on PDA medium, and place at 25°C to...

Embodiment 2

[0029] Example 2 Isolation and preparation of compound chlorinated discoidin A

[0030] Materials: Potatoes are commercially available, and other reagents used in the present invention are of analytical grade, which are sold by general biological reagent companies.

[0031] a. Activation of P.adusta SN17:

[0032] Prepare PDA medium: 200g of potatoes, 20g of glucose, 15g of agar, 1000mL of water, make a test tube slant, pick mycelium and inoculate it on the test tube slant, and incubate at 25°C for 10 days;

[0033] b. Solid fermentation culture of Discocystis fumigatus SN17:

[0034] Prepare solid medium, and its preparation process is: 150mL liquid medium (containing 6% dextrin, 2% maltose, 0.75% cottonseed meal, 0.7% peptone, 0.25% CaCO 3 , 0.25% MgSO 4 ·7H 2 O, 0.1% FeSO 4 ·7H 2 O, 0.001% ZnSO 4 , adjust the pH to 6.0) and 30g of vermiculite into a 500mL Erlenmeyer flask, prepare 10 parts, soak overnight, autoclave at 121°C for 30 minutes, and cool for later use;

...

Embodiment 3

[0040] Physicochemical analysis of embodiment 3 chlorinated discoidin A

[0041] The chlorinated discoidin A obtained in step e of Example 2 has the following physical and chemical properties: the shape is a colorless flaky crystal, and the melting point is 205-207 ° C; molecular formula: C 21 h 21 Cl 2 NO 5 ; Molecular weight: 437 (according to ESI-MS); Specific rotation [α] D +5.0(c 0.4, CH 3 OH), ultraviolet UV (CH 3 OH)λ max =211 (ε100100), 253 (ε17600), 296 (ε12200) nm. The mass spectrum and infrared spectrum data of chlorinated discoidin A are shown in Table 1, and the hydrogen spectrum and carbon spectrum data are shown in Table 2. 1 H NMR spectrum see figure 1 , 13 C NMR spectrum see figure 2 .

[0042] Table 1. Physicochemical constants of chlorinated discoidin A

[0043]

[0044] Table 2. Hydrogen spectrum and carbon spectrum data of chlorinated discoidin A (deuterated acetone)

[0045]

[0046] s-singlet; d-doublet; t-triplet; m-multiplet

[004...

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Abstract

The invention relates to the discovery of a compound with strong inhibitory activity on the growth of fusarium moniliforme and a preparation method, which belong to the technical field of biological pesticide. The compound is obtained through production strain smoky pestalotiopsis sp SN17 by adopting a solid fermentation and extraction separation method, and the structure of the compound is determined through using the technologies such as mass spectrum, nuclear magnetic resonance spectrum, infrared spectrum and the like. The compound of the invention is restalachloride A, which can effectively inhabit the growth of the fusarium moniliforme and can be singly used as the natural antagonist of the fusarium moniliforme.

Description

technical field [0001] The invention relates to the discovery and preparation of a compound chlorinated Pestalachloride A (Pestalachloride A) with strong inhibitory activity against Fusarium moniliforme, belonging to the technical field of biological pesticides. Background technique [0002] Fusarium (Fusarium Link ex Fr.) belongs to half-know fungi, including many species that endanger economic plants, not only can cause harm to wheat, rice, vegetables, etc., but also some species are pathogenic bacteria for humans and animals, and Often cause grain and feed mildew and produce toxins, causing human and animal poisoning. The sexual stage of Fusarium moniliform Sheld is Gibberella fujikuroi (Saw.) Wollenw. Can be sick. The most obvious symptom of rice bakanae disease in the field is that the diseased seedlings grow taller and thinner than healthy seedlings, the whole plant is light yellow-green, the leaves are long and narrow, and there are light red or white powdery substa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C07D209/46C12P17/10A01N43/38A01P3/00C12R1/645
Inventor 车永胜郭良栋李二伟姜立花刘述春
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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