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Streptomyces coelicolor and application thereof

A technology for strains and seeds, applied in the directions of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of low pentostatin fermentation unit and inability to industrialize, and achieve excellent fermentation performance, less fermentation by-products, and production capacity. stable effect

Active Publication Date: 2010-08-04
HANGZHOU HUADONG MEDICINE GRP PHARMA RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obtaining pentostatin in this way has a low fermentation unit and cannot be industrialized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: The culture characteristics of the bacterial strain whose preservation number is CCTCC M 208263

[0030] The strain with the preservation number CCTCC M 208263 was inoculated in the culture medium of Gao Shi No. 1, glucose asparagine, etc. for observing the morphological characteristics. Cultivate at 28°C for a certain period of time for 5, 7, and 10 days, observe and describe.

[0031] Colony characteristics of strains with preservation number CCTCC M 208263 on various media

[0032] culture medium

Embodiment 2

[0033] Example 2: Physiological and biochemical characteristics

[0034] Physiological and biochemical properties used standard methods in the classification of Streptomyces.

[0035] The physiological and biochemical reactions of the strain with the preservation number CCTCC M 208263 are as follows: gelatin can be liquefied on gelatin medium, milk can be peptonized, milk can be coagulated, starch hydrolysis is positive, cellulose is not utilized, and nitrate reduction is positive.

Embodiment 3

[0036] Example 3: Carbon source utilization

[0037] Using the method recommended by the International Streptomyces Program (ISP), the carbon source utilization experiment used ISP9 as the basal medium, cultured at 28°C for 3 to 7 days, and observed the utilization of various carbon sources tested by the strains.

[0038] When the strain with the preservation number CCTCC M 208263 grows, it can make good use of rhamnose, glycerin, glucose, raffinose, mannitol, sucrose, and inositol, and does not use sodium citrate, potassium sodium tartrate, galactose, xylose, Fructose, Arabinose.

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PUM

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Abstract

The invention discloses a streptomyces coelicolor and an application thereof. The bacterial strain thereof is collected by China Center for Type Culture Collection with the collection number of CCTCC No. M 208263 and the collection date is 26th December, 2008. The invention also discloses a method for preparing pentostatin by fermenting the bacterial strain. The fermentation process provided by the invention is tested by a 10 ton fermentation tank, has stable production capacity, high fermentation unit and fewer fermentation by-products, thus lowering the difficulty for follow-up extraction and being suitable for industrialized production.

Description

technical field [0001] The invention relates to a novel microorganism and its application, in particular to a streptomyces and its application in the preparation of pentostatin. Background technique [0002] Pentostatin is an anti-metabolite of purines, and its anti-tumor effect is realized by inhibiting adenosine deaminase (ADA) in the body. ADA is an enzyme involved in the purine rescue metabolic pathway, which catalyzes the irreversible deamination of intracellular adenosine and deoxyadenosine, thereby controlling the intracellular adenosine level. Pentostatin inhibits the activity of ADA, resulting in a large accumulation of deoxyadenosine and 5'-triphosphate deoxyadenosine (dATP), and the accumulation of dATP will eventually lead to cell death. This process may be related to DNA or RNA synthesis is inhibited. [0003] The indications for pentostatin are: 1. stem cell leukemia (hairy cell leukemia, HCL); 2. chronic lymphocytic leukemia; 3. mycosis fungoides. [0004] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/38C12R1/465
Inventor 朱健陈晓霞谢祥茂陈俊勇
Owner HANGZHOU HUADONG MEDICINE GRP PHARMA RES INST
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