Tri-union compound immunopotentiator and method for preparing same
A technology of immune enhancer and immunoglobulin, which is applied in the field of triple compound immune enhancer and its preparation, can solve the problems that have not been reported, and achieve the effects of reasonable compatibility, protection of the body's immune function from damage, and wide application fields
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[0035] The present invention is described below in conjunction with accompanying drawing and specific embodiment is as follows:
[0036] 1. Take fresh pig blood (it can also be cow blood or sheep blood), add anticoagulant, centrifuge, and collect plasma and blood cells respectively.
[0037] 2. Add ammonium sulfate to the blood plasma by volume to a saturation of 60%. After the ammonium sulfate is completely dissolved, let it stand for 2 hours, filter or centrifuge, collect the filtrate (I) and the precipitate, and the precipitate is desalted by dialysis to obtain the crude immunoglobulin solution ;
[0038] 3. The filtrate (I) is first passed through an ultrafiltration device system with a molecular weight cut-off of 6000, and the retentate (I) and the dialysate (I) are collected respectively.
[0039] 4. Pass the dialysate (I) through an ultrafiltration device system with a molecular weight cut-off of 1000, and collect the retentate and dialysate (II) respectively, and the ...
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