Modified phanerochaete chrysosporium adsorbent as well as preparation and application thereof
A technology of P. protothecoides and adsorbents, which can be used in adsorption water/sewage treatment, other chemical processes, chemical instruments and methods, etc., can solve the problems of limitation, low reaction efficiency, long action time, etc., and achieve easy control, adsorption and adsorption. Large capacity, no secondary pollution effect
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Embodiment 1
[0022] Scrape the spores of Phanerochaete chrysosporium on the plate into sterile water to form a spore suspension, then add the quantitative suspension to the liquid medium, shake and cultivate at 35°C and about 120r / min for 2~ 3 days. Filter, collect mycelial balls, take out mycelial balls and bake at 40°C for 4 hours. Mix the dried 10g of Phanerochaete chrysosporium, 3ml of azobenzene, 100ml of chloroform and 10ml of 4-bromobutyryl chloride, wherein 4-bromobutyryl chloride is added dropwise to the mixed solution in the last step, and the reaction mixture Seal, shake slowly at 25°C for 12 hours, filter, and then rinse the filtered Phanerochaete chrysosporium with chloroform to wash away unreacted 4-bromobutyryl chloride, and then wash the washed Chrysosporium Phanerochaete is immersed in a modified solution, which is prepared by mixing 10g polyethyleneimine, 0.1gKOH and 100ml tert-amyl alcohol, wherein the amount of polyethyleneimine is the same as that of the initial addit...
Embodiment 2
[0026] The spores of Phanerochaete chrysosporium on the plate were scraped into sterile water to form a spore suspension, and then the quantitative suspension was added to the potato medium (the potato medium was sterilized at 105°C), and the Under the conditions of 35° C. and about 120 r / min, shaking culture was carried out for 2 to 3 days, and Phanerochaete chrysosporium mycelium balls were cultivated. The mycelial balls were collected by filtration, and the mycelial balls were taken out and baked at 40°C for 4 hours.
[0027] Put 10g of dried Phanerochaete chrysosporium in 2.5mL of azobenzene and 95mL of chloroform, then add 5mL of 4-bromobutyryl chloride dropwise, seal the reaction mixture, and slowly Shake for 12 hours, filter, and then rinse the filtered Phanerochaete chrysosporium with chloroform to wash away unreacted 4-bromobutyryl chloride, and then immerse the washed Phanerochaete chrysosporium in a solution containing 10 g of polyethylene imine, 0.1g KOH and 90mL ...
Embodiment 3
[0029] The modified Phanerochaete chrysosporium adsorbent prepared in Example 2 was applied to the purification treatment of heavy metal wastewater containing hexavalent chromium, and the purification treatment effect under different pH values was investigated.
[0030]Add 0.05 g of the above-mentioned modified Phanerochaete chrysosporium adsorbent to 100 mL of chromium-containing wastewater with a concentration of 200 mg / L, adjust the pH value of the chromium-containing wastewater with NaOH and HCl, and prepare a pH value of 1 to 9.5 For a series of chromium-containing wastewater samples that vary within a certain range, maintain a temperature environment of 25°C on an oscillator with a temperature adjustment device, shake at 120r / min for 6 hours, take the supernatant, and measure the residual Cr(VI) in the solution , the measurement results are shown in Table 1, and the effect of pH value on the adsorption effect is shown in image 3 shown.
[0031] Table 1: Adsorption ca...
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