Tissue cultivating method of dendrobium officinale growing in Yunnan

A technology of tinplate and tissue cultivation in Yunnan, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of restricting the production and development of Dendrobium candidum test-tube seedlings, shortage of raw materials, long breeding time, etc., to achieve sustainable development. The effect of utilization and development, ensuring the quality of seedlings, and shortening the growth cycle

Inactive Publication Date: 2010-09-08
KUNMING YOULIFENGYUAN TECH DEV
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  • Application Information

AI Technical Summary

Problems solved by technology

Due to the small scale and low output of the existing artificial planting, it cannot meet the market demand at all, so the expensive raw materials are extremely scarce, and a large number of counterfeit Dendrobium officinale appear
Due to problems such as low protocorm differentiation rate of Dendrobium candidum, low multiplication coefficient, long propagation time, and high production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: explant culture

[0016] The robust dormant buds of Dendrobium candidum were inoculated on MS sucrose agar medium containing auxin, cytokinin, 200ml / L coconut milk and 3% activated carbon after being sterilized by alcohol and mercuric chloride, and cultured in a light culture room for 2~ After 3 weeks, most of them can germinate and grow new shoots. They have a higher germination rate and good growth in the medium of NAA0.5mg / L+6-BA 1.0-2.0mg / L. After 2 months, the seedlings Up to 1.5cm-3.0cm.

Embodiment 2

[0017] Embodiment 2: protocorm induction and cultivation thereof

[0018] Use the tissue culture seedlings that have established a sterile system, cut the stems into small pieces and continue to induce the differentiation of protocorms, add 100ml / L coconut milk in the range of 0.2-0.5mg / L NAA and 1.0-2.0mg / L 6-BA hormone On the MS sucrose agar medium, after being cultured in a light culture room for 1 month, protocorms grow from around the mother body, the differentiation frequency is 100%, and the proliferation coefficient is more than 10 times. According to this method, subculture every one month, the annual proliferation coefficient of each bud is theoretically 1.0×10 10 above.

Embodiment 3

[0019] Embodiment 3: lateral bud differentiation and subculture thereof

[0020] Cut the above-mentioned seedlings into leafy stems of 1~2cm in length, and inoculate them on MS sucrose agar medium containing auxin 0.2-0.8mg / L NAA, cytokinin 1.0-2.5mg / L BA, and 100ml / L coconut milk Above, after one and a half months of cultivation in the light incubator, axillary buds grow from the leaf axils of the stem segment, the differentiation frequency is 100%, and the proliferation coefficient is more than 2 times. According to this method, subculture every one and a half months, the annual proliferation coefficient of each bud is theoretically 1.9×10 4 above.

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PUM

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Abstract

The invention relates to a tissue cultivating method of dendrobium officinale growing in Yunnan. In the tissue cultivating method, wild dendrobium officinale high-quality plants grown in Yunnan are selected and explants are vigorous dormant buds and mature fruits. Main agents are large amount of elements, trace elements, ferric salt, inositol, glycine, praline, various vitamins and plant hormone. The dendrobium officinale is cultivated under the conditions that the temperature is 25+/-2DEG C, the illumination time is 12 hours and the illumination intensity is 1,000-2,000muMm<-2>s<-1>. The invention breaks through the limit of seedling cultivation on propagating materials, seasons, places and the like. The method can ensure the consistency of the quality of seedlings and high-quality of seed sources of traditional Chinese plant of dendrobium officinale, solve the bottleneck difficult problems that protocorm of the dendrobium officinale has low differentiation rate and low proliferation and test tube seedlings have weak growth and has the characteristics of short growth period, low production cost, high quality and scale production of seedlings.

Description

technical field [0001] The invention relates to a tissue cultivation method of Dendrobium officinale in Yunnan. Background technique [0002] Dendrobium officinale is a traditional precious Chinese medicinal material with a medicinal history of more than 2,000 years. The original plant is Dendrobium officinale (Dendrobium officinale). After being listed as endangered plants in the "Convention on International Trade in Endangered Wild Fauna and Flora", my country has strictly restricted and managed the excavation, purchase, and management of Dendrobium. Wild medicinal Dendrobium resources are scarce and grow slowly, and it has gradually become a rare species that is on the verge of extinction. endangered species. Due to the small scale and low output of the existing artificial planting, it cannot meet the market demand at all, so the expensive raw materials are extremely scarce, and a large number of counterfeit Dendrobium officinale appear. Due to problems such as low proto...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈昌祥张艳张抱琼熊云昆黄曦吴超侯军刘杨超
Owner KUNMING YOULIFENGYUAN TECH DEV
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