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Method for separating and purifying protein from corn starch wastewater

A technology of corn starch and waste water, which is applied to the preparation method of peptides, chemical instruments and methods, extraction water/sewage treatment, etc., can solve the problems of separation and purification of protein that have not been reported, and achieve strong operability, high recovery rate, The effect of simple process equipment

Active Publication Date: 2010-09-08
山东汇盛天泽环境工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the 1980s, domestic research on two-phase extraction technology has also been carried out, but there is no report on the separation and purification of protein from starch wastewater.

Method used

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  • Method for separating and purifying protein from corn starch wastewater
  • Method for separating and purifying protein from corn starch wastewater
  • Method for separating and purifying protein from corn starch wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1 Experimental method:

[0015] In this experiment, PEG (polyethylene glycol)-(NH 4 ) 2 SO 4 A two-phase system was used to separate and purify the protein in corn starch wastewater. The separation effect of the two-phase system on protein under different influencing factors (polymer and its molecular weight, pH value, temperature, ion concentration, etc.) was analyzed.

[0016] 1.1 Experimental materials

[0017] Contains: polyethylene glycol with molecular weights of 3000, 6000 and 8000, ammonium sulfate ((NH 4 ) 2 SO 4 ), bovine serum albumin. All reagents required were of analytical grade, and neither polyethylene glycol nor ammonium sulfate required further purification. The water used throughout the experiment was ultrapure water.

[0018] 1.2 Preparation of samples

[0019] The water samples used in this experiment all come from the corn soaking solution of Henan Yongchang Feitian Starch Co., Ltd., and the protein content of the corn soaking solution is ...

Embodiment 2

[0060] (1) Extraction of object product: the cornstarch homogenate waste water that has added 0.05mol / L phosphate buffer pH value and is adjusted to 10, contains protein 7% (wt / wt), the concentration that molecular weight is 3000 is 30% (wt) / wt) polymer PEG and a concentration of 21% (wt / wt) NH 4 SO 4 Mix in the homogeneous slurry mixing tank, the corn starch wastewater is mixed with PEG, NH 4 SO 4The volume ratio is 1:2:2; form a homogeneous slurry, and then enter the separator for separation, the target protein is separated to the lower phase (PEG phase), and other impurities such as nucleic acid, polysaccharide, etc. are distributed to the upper phase (salt-rich phase); the second The first step extraction is to transfer the target protein into the salt-rich phase. The method is to add a NaCl aqueous solution with a concentration of 1.0M to the lower phase, form a new two-phase system with the lower phase at a volume ratio of 1:1, and then enter the separator for separat...

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Abstract

The invention discloses a method for separating and purifying protein from corn starch wastewater. The method comprises the following steps of: mixing the corn starch wastewater with pH value of between 8 and 10 adjusted by adding phosphoric acid, polymer PEG and NH4SO4 in a homogenizing serous fluid mixing pool; forming homogenizing serous fluid, allowing the serous fluid to enter a separator for separating, and separating target protein to a lower phase; adding NaCl aqueous solution in the lower phase to form a new aqueous two-phase system with the lower phase; and allowing the aqueous two-phase system to enter the separator for separating, separating the target protein to an upper phase, distributing other impurities to the lower phase, and further concentrating and drying the upper phase containing the protein to obtain the product protein. The method has simple equipment and strong operability, and the added polymer and salt can be reclaimed and recycled. The method has high reclamation rate, and cannot cause secondary pollution to the environment. By using the method, not only the protein in the corn starch wastewater can be separated, but also the protein in other starch wastewater can be separated and purified.

Description

technical field [0001] The invention relates to a method for separating and purifying protein from cornstarch wastewater. Background technique [0002] With the continuous expansion of domestic corn starch production scale, corn starch wastewater is constantly increasing. Corn starch wastewater is mainly composed of two parts, corn soaking liquid and production process water. The CODcr content of corn starch wastewater is high. If it is discharged directly, it will not only pollute the water environment, increase the cost of wastewater treatment for enterprises, but also waste the protein in it. [0003] The traditional process is to separate the protein after the soaking liquid is concentrated from 6-7% protein (wt / wt) to 40-48% by three-effect or four-effect evaporation. However, the cost of three-effect evaporative concentration or four-effect evaporative concentration is relatively high, and the energy consumption is also high. Take an enterprise with an annual output...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C02F1/26
Inventor 于桂英张喜玲梅媛
Owner 山东汇盛天泽环境工程有限公司