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Method for extracting kaempferol

An extraction method and technology for kaempferol, applied in the field of extraction of kaempferol, can solve the problems of poor solubility, less extraction of kaempferol, product loss, etc., and achieve the effects of improving the utilization rate of raw materials, improving the extraction rate and saving energy

Inactive Publication Date: 2010-09-29
苏州北商智业管理咨询有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] There are very few literatures on the extraction of a single component of kaempferol, and the research is not deep enough
Cao Mingcheng's "Study on the Extraction Process of Thyme Grass" studied a method of water extraction and alcohol precipitation, because the solubility in water is not good, and the loss in the extraction process is relatively large
Chinese patent: CN10115303 "A method for extracting active ingredient kaempferol in ginkgo leaves by ultrasonic wave" mentioned method drying process is too long, which easily causes product loss

Method used

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  • Method for extracting kaempferol

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Effect test

Embodiment 1

[0030] Grind 10kg of raw materials (2.4% equivalent content of kaempferol and its derivatives), add 80L of 1% lime water, stir and heat in a boiling water bath for 1.5 hours, cool; add phosphoric acid to the filtrate to adjust pH to 5, filter, add 50g of fiber Suzyme, heat up to 40°C in a stirring heating device, stabilize for 15 hours and hydrolyze to obtain 84L hydrolyzate; heat and concentrate the hydrolyzate to 8L, add ethyl acetate to stir and extract, take the ester phase and recover ethyl acetate under reduced pressure to obtain 487g Extraction: use 2L 95% methanol for the extract, and finally obtain 210g of kaempferol finished product with a content of 99.1%.

Embodiment 2

[0032] Grind 10kg of raw materials (the equivalent content of kaempferol and its derivatives is 3.1%), add 90L of 1.5% lime water, stir and heat in a boiling water bath for 1.7 hours, and cool; add phosphoric acid to the filtrate to adjust the pH to 6, filter, and add 75g of fiber Suzyme, heat up to 45°C in a stirring heating device, stabilize for 18 hours and hydrolyze to obtain 95L hydrolyzate; heat and concentrate the hydrolyzate to 8L, add ethyl acetate to stir and extract, take the ester phase and recover ethyl acetate under reduced pressure to obtain 496g Extract: 2.5L 85% methanol is used for the extract to finally obtain 274g of kaempferol finished product with a content of 98.3%.

Embodiment 3

[0034] Grind 10kg of raw materials (2.7% equivalent content of kaempferol and its derivatives), add 100L of 1.5% lime water, stir and heat in a boiling water bath for 2 hours, cool; add acetic acid to the filtrate to adjust the pH to 7, filter, add 100g of fiber Suzyme, heat up to 50°C in a stirring heating device, stabilize for 20 hours and hydrolyze to obtain 107L hydrolyzate; heat and concentrate the hydrolyzate to 7L, add ethyl acetate to stir and extract, take the ester phase and recover ethyl acetate under reduced pressure to obtain 491g Extract: use 3L 80% methanol for the extract, and finally obtain 243g of kaempferol finished product with a content of 98.5%.

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Abstract

The invention relates to a method for preparing kaempferol. The method comprises the following technical steps of: crushing raw materials, and extracting alkaline solution; performing enzymolysis after acid adjustment, and extracting enzymolysis liquid by using ethyl acetate; and performing concentration under reduced pressure on the extract liquor, and recrystallizing by using methanol to obtain the product. The method for producing the kaempferol has the advantages of simple process, high utilization ratio of raw materials, and high purity.

Description

Technical field: [0001] The invention relates to a method for extracting kaempferol, in particular to a method for purifying kaempferol by enzymolysis and recrystallization. Background technique: [0002] Kaempferol [0003] Alias: kaempferol-3, kaempferol, kaempferol, tetrahydroxyflavone, thymosin III. [0004] English name: Kaempferol [0005] Molecular formula: C 15 h 10 o 6 [0006] Molecular weight: 286.23 [0007] Molecular Structure: [0008] [0009] Ingredient classification: flavonols [0010] Physical properties: yellow needle crystal, melting point 276°C-278°C, sensitive to light and air. Kaempferol is slightly soluble in water, soluble in hot ethanol, ether and alkali. [0011] Pharmacological action: Antibacterial, has inhibitory effect on Staphylococcus aureus, Pseudomonas aeruginosa, typhoid bacillus and Shigella. Cough, treatment of bronchitis. Inhibits enzymes, inhibits eye aldose reductase, and is beneficial to the treatment of diabetic cata...

Claims

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Application Information

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IPC IPC(8): C12P17/06C07D311/30C07D311/40
Inventor 孙世芳赵玲
Owner 苏州北商智业管理咨询有限公司
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