Method for producing swine fever live vaccine by using porcine kidney cell line (PK15) and product thereof

A technology of swine fever live vaccine and pig kidney cells, which is applied in the field of production of swine fever live vaccine with pig kidney cell lines, can solve the problems of vaccine production and efficacy improvement, cell exogenous virus contamination, low toxin titer and other problems , to achieve good economic benefits and application prospects, to solve the pollution of exogenous pathogens from cattle, and to be easy to operate

Inactive Publication Date: 2010-10-06
广东永顺生物制药股份有限公司 +1
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Problems solved by technology

Since BVD / MDV disease is relatively common in cattle herds in my country, the use of primary cells of bovine testis to produce vaccines may easily cause cell exogenous virus contamination, and the titer of toxin production is not high, and there are large batch-to-batch differences, which will bring great benefits to the improvement of vaccine production and efficacy. difficulty

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  • Method for producing swine fever live vaccine by using porcine kidney cell line (PK15) and product thereof
  • Method for producing swine fever live vaccine by using porcine kidney cell line (PK15) and product thereof

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Embodiment 1

[0023] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. Embodiment 1 produces the method for swine fever live vaccine with porcine kidney cell line (PK15)

[0024] (1) Selection of cells for seedling preparation: select porcine kidney (PK15) cell line for use;

[0025] (2) Passage and culture of cells for seedling production: PK15 cell line was digested and passed by EDTA-trypsin cell dispersion liquid, and continued to culture at 37°C with cell growth liquid. When a good monolayer was formed, it was used for continued passage or virus inoculation;

[0026] (3) Propagation of cytotoxic species: use cell maintenance solution to prepare 0.5% virus suspension from fresh spleen venom, inoculate a monolayer of well-growing PK15 cell line, and place at 37° C. to continue culturing. Harvest and change the medium every 4 days, take the second harvest and third harvest of cell culture venom as the poisonous s...

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Abstract

The invention discloses a method for producing a swine fever live vaccine by using a porcine kidney cell line (PK15). The method comprises the following technical steps of: (1) selecting a porcine kidney cell line (PK15) as a cell for producing the vaccine; (2) carrying out sub-culturing on the cell for producing the vaccine; (3) reproducing virus cell seeds; (4) reproducing venom for producing the vaccine; and (5) preparing the vaccine, and performing split charging and freeze-drying. The method of the invention has the advantages of simple and stable production process, easy operation, high content of virus, small difference between bitches, and controllable quality, and capability of improving the quality and yield of the vaccine. The swine fever live vaccine produced by the method of the invention has high safety and high immune effect, and has a complete immune protection function on the attacks of the strong swine fever viruses.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and more specifically relates to a method for producing a live swine fever vaccine by using a pig kidney cell line (PK15). Background technique [0002] The cells used in the production of attenuated CSF vaccine in my country are primary bovine testicular cells. Since BVD / MDV disease is relatively common in cattle herds in my country, the use of primary cells of bovine testis to produce vaccines may easily cause cell exogenous virus contamination, and the titer of toxin production is not high, and there are large batch-to-batch differences, which will bring great benefits to the improvement of vaccine production and efficacy. difficulty. Contents of the invention [0003] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a method for producing live swine fever vaccine with porcine kidney cell line (PK15). The method has the...

Claims

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Application Information

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IPC IPC(8): A61K39/187A61P31/14
Inventor 吴文福宁宜宝林旭埜张毓金岑小清游启有张国丽任向阳杨傲冰张木辉司徒剑谋
Owner 广东永顺生物制药股份有限公司
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