Method and kit for synchronously cultivating bacterium group

Abstract

The invention relates to the field of medicaments, in particular to a method for synchronously cultivating a bacterium group and a kit for synchronously cultivating the bacterium group. The kit of the invention comprises aerobe group cultivation proliferation solution, anaerobe group cultivation proliferation solution, bacteria L-form group cultivation proliferation solution and cell bacterium group cultivation proliferation solution. The method and the kit of the invention have the advantages of necessary sample amount for cultivating bacteria of only 1 to 2 percent of an ordinary using amount, positive detection rate of over 95 percent, capacity of judging a result within 6 to 8 hours of bacterium cultivation, obtainment of pathogenic bacterium group medicament sensitive data within 24 hours, accordance with the basic rule of evidence-based diagnosis of evidence-based medicament and wide clinical application prospect.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for synchronous culture of bacterial groups, explanation of direct drug susceptibility test results, definition of technical standards and a kit. Background technique [0002] Clinical bacterial culture is a commonly used clinical bacterial pathogenic (cause) inspection method in hospitals, that is, to provide the nutrients and environment necessary for bacterial growth by artificial means, so that the clinical pathogenic bacteria in the human body can reproduce in a special medium , and then according to the biological characteristics of the colony's shape, growth characteristics, special needs for nutrition and changes in indicators to judge the bacterial category, identify the bacterial species, and guide clinical medication, which has high application value. [0003] In recent years, due to the widespread use of antibiotics, drug-resistant strains have gradually eme...

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Problems solved by technology

[0007] 1. Large sampling volume: Taking blood samples as an example, 5-10 ml of blood is required for each culture. If four types of bacterial culture are performed at the same time, 20-40 ml of blood is required. Many patients are unwilling to accept it, especially newborns. Bacterial culture for infectious diseases;

[0008] 2. The bacterial culture report takes a long time, and the results can only be produced within 7 days; the drug sensitivity report takes 72 hours;

[0009] 3. The positive detection rate is low, and the positive detection rate of aerobic fungi culture is less than 13%;

[0010] 4. Neglecting that the mixed infection of clinical pathogenic bacteria is an important objective phenomenon of clinical pathogenic bacteria, and ignoring the actual state of continuous mutation of clinical pathogenic bacteria

[0011] 5. First cultivate aerobic bacteria, if there is no growth, then cultivate anaerobic bacteria, if there is no growth, then cultivate other bacteria, delaying the treatment time

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