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Glucosamine modified poly (ethylene glycol) diacrylate (PEGDA) hydrogel, preparation method and application thereof

A glucosamine and polyethylene glycol double technology is applied in the field of glucosamine modified polyethylene glycol diacrylate hydrogel and its preparation, which can solve the problem of lack of biological activity and cell recognition signal, unfavorable cell-specific gene activation, etc. problem, to achieve the effect of being conducive to large-scale production, good biocompatibility, and increasing secretion

Active Publication Date: 2012-09-26
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

PEG is a biomedical material approved by the US FDA for clinical use. Although PEG diacrylate gel is a biocompatible material, it lacks biological activity and cell recognition signals, which is not conducive to cell-specific gene activation.

Method used

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  • Glucosamine modified poly (ethylene glycol) diacrylate (PEGDA) hydrogel, preparation method and application thereof
  • Glucosamine modified poly (ethylene glycol) diacrylate (PEGDA) hydrogel, preparation method and application thereof
  • Glucosamine modified poly (ethylene glycol) diacrylate (PEGDA) hydrogel, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0040] The preparation of glucosamine modified PEGDA hydrogel comprises the following steps:

[0041] (1) Synthesis of polyethylene glycol diacrylate

[0042] Weigh 50 grams of polyethylene glycol (number average molecular weight 4000), dissolve it in 700ml toluene, heat to reflux, remove the water content in the raw material with a water separator, cool to room temperature, under the protection of high-purity nitrogen, add 0.1 Mole of anhydrous triethylamine, 0.1 mole of acryloyl chloride was added dropwise, and stirred at 35°C for 24 hours. Remove the precipitate generated by the reaction by filtration, add 300 ml of diethyl ether to the filtrate and place it in a refrigerator at 4°C to precipitate a white precipitate, filter it, redissolve the precipitate in 100ml of benzene, add 200 ml of ether to the refrigerator at 4°C, precipitate a white precipitate, and repeat the filtration and dissolved twice, and the resulting white precipitate was dried in a vacuum oven at 35°C f...

Embodiment 2

[0048] (1) Synthesis of polyethylene glycol diacrylate

[0049] Weigh 50 grams of polyethylene glycol (number-average molecular weight 10000), dissolve it in 700ml toluene, heat to reflux, remove the water content in the raw material with a water separator, cool to room temperature, under the protection of high-purity nitrogen, add 0.04 Mole of anhydrous triethylamine, 0.04 mole of acryloyl chloride was added dropwise, and stirred at 35°C for 24 hours. Remove the triethylamine salt generated by the reaction by filtration, add 300 ml of ether to the filtrate and place it in a refrigerator at 4°C, a white precipitate precipitates, filter, redissolve the precipitate in 100ml of benzene, add 200ml of ether and place it in a refrigerator at 4°C, a white precipitate precipitates, Filtration and dissolution were repeated twice, and the resulting white precipitate was dried in a vacuum oven at 35°C for 24 hours.

[0050] (2) Synthesis of N-acryloylglucosamine: Dissolve 8.6g of glucos...

Embodiment 3

[0056] (1) Synthesis of polyethylene glycol diacrylate

[0057] Weigh 50 grams of polyethylene glycol (number-average molecular weight 10000), dissolve it in 700ml toluene, heat to reflux, remove the water content in the raw material with a water separator, cool to room temperature, under the protection of high-purity nitrogen, add 0.04 Mole of anhydrous triethylamine, 0.04 mole of acryloyl chloride was added dropwise, and stirred at 35°C for 24 hours. Remove the triethylamine salt generated by the reaction by filtration, add 300 ml of ether to the filtrate and place it in a refrigerator at 4°C, a white precipitate precipitates, filter, redissolve the precipitate in 100ml of benzene, add 200ml of ether and place it in a refrigerator at 4°C, a white precipitate precipitates, Filtration and dissolution were repeated twice, and the resulting white precipitate was dried in a vacuum oven at 35°C for 24 hours.

[0058] (2) Synthesis of N-acryloylglucosamine: Dissolve 8.6g of glucos...

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Abstract

The invention discloses a glucosamine modified poly (ethylene glycol) diacrylate (PEGDA) hydrogel, a preparation method and application thereof. The glucosamine modified poly (ethylene glycol) diacrylate hydrogel contains 10-30 percent of poly (ethylene glycol) diacrylate, 1.0-10mM of acryloyl or propenyl glucosamine and 0.03-0.1 percent of initiating agent I2959. The preparation method comprisesthe steps of: preparing the PEGDA by using polyethyleneglycol as a basic material; preparing the acryloyl or propenyl glucosamine by using glucosamine hydrochloride as a raw material; and preparing awater solution or PBS (Poly (Butadiene-Styrene) solution by using the acryloyl or propenyl glucosamine and the initiating agent I2959 in a certain proportion, and can be cured into the hydrogel afterbeing irradiated by ultraviolet with 365nm wavelength. The hydrogel can be used for tissue repair of clinical medicine, has simple preparation process, can realize in situ injection forming, meet therequirement on minimally invasive procedure, and has better application prospect and scientific significance.

Description

technical field [0001] The invention relates to a modified biological material and its preparation technology, in particular to a glucosamine modified polyethylene glycol diacrylate hydrogel with good biocompatibility and biological activity, its preparation method and application. Background technique [0002] Glucosamine is a structural unit of glycosaminoglycan, hyaluronic acid, which constitutes the extracellular matrix of cartilage. Glucosamine hydrochloride or sulfate, as a clinical drug for the treatment of osteoarthritis, can relieve the pain of patients and reduce the narrowing of the joint cavity. Although its pharmacological effects are still unclear, in vitro experiments have shown that a certain concentration (2mM added to the medium) of glucosamine can promote the secretion of proteoglycan and collagen II in three-dimensional cultured chondrocytes, and can up-regulate the expression of TGFb-1 in chondrocyte mRNA ; Can promote three-dimensional culture of embry...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F290/06C08G65/48A61L27/16A61L27/52
Inventor 王迎军任力王群芳赵连娜夏欢
Owner SOUTH CHINA UNIV OF TECH
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