New fermentation method

Abstract

The invention discloses a new fermentation method, which mainly comprises the following steps of: (1) preparing liquid seeds; (2) preparing a solid plate: adding 18 to 32g of agar into each 1,000 ml of corresponding liquid fermentation culture medium for strict anaerobes, sterilizing, pouring into a plate, cooling, and preparing a solid plate culture medium containing liquid culture medium components by utilizing the solidification capacity of the agar on liquid; and (3) performing amplified fermentation: spraying the corresponding liquid seeds obtained by the step (1) to the surface of the solid plate uniformly, putting the sprayed solid plate in a fermentation chamber at the constant temperature of between 35 and 37 DEG C for closed fermentation culture, culturing for 24 to 72 hours according to different bacterial strains, and collecting bacterial sludge. The method has the advantages of low cost, simple operation, easy subsequent processing and suitability for the production of most of microbial bacterial strains; and through the method, products which break through the upper limit of bacterial concentration in the conventional microbial preparation products greatly can be produced, and about 1,000 billion / g bacterial powder products can be obtained.

Description

technical field [0001] The invention belongs to the field of microorganism cultivation, and in particular relates to a novel fermentation method of microorganisms. Background technique [0002] At present, in industrial fermentation, the main fermentation processes can be classified into solid-state fermentation and liquid fermentation. [0003] Liquid fermentation is a culture in which bacteria are inoculated into a fermenter, the bacteria cells are suspended in a liquid fermentation medium, and the growth is carried out by controlling parameters such as temperature, stirring, air, pressure, and nutrient concentration. method. Submerged liquid culture generally requires air and stirring, which is a traditional microbial fermentation culture method. It mainly has the disadvantages of expensive equipment, complex operation, low concentration of bacterial cells produced per unit culture medium, and high cost of bacterial liquid separation. [0004] Solid fermentation includ...

AI Technical Summary

Problems solved by technology

[0013] 1. The sterilization and disinfection of materials is difficult to do thoroughly, and the cost is high, which can easily cause bacterial contamination and affect product quality

[0014] 2. It is limited to microorganisms that grow in low humidity conditions, so the possible processes and products are more likely to be restricted, and generally more suitable for fungi

[0015] 3. It is not easy to evenly distribute substances and energy by stirring, so during the fermentation period, the addition of substances cannot be uniform, and the fermentation effect is not uniform.

[0016] 4. Fermentation in a relatively dense environment often causes problems with heat dissipation, and the lower part of the material is often insufficiently ventilated. In the case of uneven stirring, the growth of oxygen-consuming bacteria is not good, especially in mass production, which often limits its size. Scale capacity

[0017] 5. It is not easy to extract pure bacteria, often mixed with the carrier

[0018] 6. The subsequent drying process usually uses hot air drying, which causes a large loss in the number of viable bacteria

[0019] 7. The order of magnitude of the bacteria content of the finished product generally does not exceed about 100 billion / g, and it is usually difficult to reach about 100 billion / g