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Method for preparing gallic acid

A gallic acid and gallic acid technology, applied in the field of preparation of gallic acid, can solve the problems of unsafe hydrolysis, easy volatilization of ammonia water, and insufficient yield, and achieve the effects of easy treatment, low pollution of "three wastes" and high yield of products

Inactive Publication Date: 2010-10-20
遵义市倍缘化工有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, gallnut and tara powder are used as raw materials, and ammonia water is used to adjust the pH value in the process of enzyme production and acid production. Due to the shortcomings of ammonia water, which is volatile and strong in taste, it pollutes the environment.
There is no microbial growth and enzyme production process in the acid production stage, the amount of enzyme used is large, and the production of enzyme and acid production requires more than 40 hours. The hydrolysis is not safe enough, and the yield is not high enough.

Method used

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  • Method for preparing gallic acid
  • Method for preparing gallic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Prepare culture medium according to the following components (g / l)

[0015] (NH 4 ) 2 SO 4 2

[0016] K H 2 PO 4 2

[0017] MGSO 4 7H 2 O 1

[0018] Zn 4 0.6

[0019] yeast paste 1

[0020] Sucrose 15

[0021] Tannic acid 100

[0022] Divide the prepared medium into several 1000ml Erlenmeyer flasks for sterilization. In order to avoid high-temperature oxidation of tannins, tannic acid was added after sterilization to adjust the initial pH value of the solution to 4.0 with 10mol / L NaOH; then immediately inoculated with Aspergillus niger NO.316, and placed on a ZHWY-2102C constant temperature oscillator for reaction. Set the temperature at 23-38°C, adjust the rotation speed at 120-128r / min, adjust it several times with 10mol / L NaOH, keep the pH value of the reaction solution at about 3.0-5.5, react continuously for 30h, and measure the enzyme activity or tannin The acid residue changes slowly, that is, it is used to make acid.

Embodiment 2

[0024] Prepare medium (g / L) according to the following components:

[0025] NH 4 cl 2

[0026] K H 2 PO 4 2

[0027] MgSO 4 7H 2 O 1

[0028] ZnSO 4 0.6

[0029] yeast paste 1

[0030] Glucose 15

[0031] Tannic acid 100

[0032] Vitamin B complex 8mg / L

[0033] The prepared medium was divided into several 1000ml Erlenmeyer flasks, and tannin was added after sterilization. Remaining steps are carried out according to enzyme production embodiment 1.

[0034] Acid-making embodiment:

[0035] Prepare medium (g / L) according to the following components:

[0036] (NH 4 ) 2 SO 4 2

[0037] K H 2 PO 4 2

[0038] MgSO 4 7H 2 O 1

[0039] ZnSO 4 0.6

[0040] yeast paste 1

[0041] Put the prepared medium into the fermenter equipped with a semi-automatic feeding alkali device and an external pH meter and dissolved oxygen meter, and sterilize.

[0042] Tannic acid is added to dissolve, so that the concentration of tannin ...

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Abstract

The invention discloses a method for preparing gallic acid. In the method, enzyme preparation and acid preparation processes are performed in two different containers by stages; and NaOH solution is used for adjusting pH value in both the enzyme preparation and acid preparation processes. The invention aims to provide a method for preparing gallic acid, which reduces reaction time and raw and auxiliary material consumption, overcomes the drawbacks of strong smell, effumability and easy environmental pollution of the ammonia water due to the fact that the pH value is adjusted not by ammonia water and reduces environmental pollution. And as microorganism growing and enzyme production processes are still performed in the acid production period, the method is advantageous over a common enzyme method in hydrolysis safety, and product purity and yield and can create great social and economic benefits.

Description

technical field [0001] The invention relates to a method for preparing gallic acid, which uses industrial tannic acid as a raw material and adopts a whole-cell biocatalysis method to prepare gallic acid, belonging to the field of biotechnology. Background technique [0002] Gallic acid (Gallic Acid, GA) is also known as beilic acid and gallic acid, and its chemical name is 3,4,5-trihydroxybenzoic acid. It is white or light yellow needle crystal or powder, usually in the form of a hydrate exist. As an important chemical product, gallic acid has a broad application market; it can be used in organic synthesis, coatings, dyes, medicine, food, chemical industry, leather, daily chemicals, agriculture, minerals and other fields, mainly used in drugs, dyes, Food, cosmetics, feed and other fields. In addition, there is also a certain application market in semiconductor photosensitive resin. [0003] The traditional production process of preparing gallic acid: use gallnut and tara ...

Claims

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Application Information

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IPC IPC(8): C12P7/42C12R1/685
Inventor 张基明邓厚璋王石发李迅谷文
Owner 遵义市倍缘化工有限责任公司
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