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Method for extracting paeoniflorin from white paeony root by enzyme process

A technology of paeoniflorin and white peony, applied in the field of enzymatic extraction of paeoniflorin from white peony, can solve the problems of large solvent consumption, long process, low yield, etc., and achieve the advantages of reduced solvent consumption, low operating temperature and short process Effect

Inactive Publication Date: 2010-11-03
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is to provide a method for enzymatically extracting paeoniflorin from Radix Paeoniae Alba, using Radix Paeoniae Alba as a raw material, using enzymatic extraction and separation to overcome the long process and large solvent consumption in the prior art , high temperature and low yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for enzymatically extracting paeoniflorin from Radix Paeoniae Alba, comprising the following steps in sequence,

[0025] (1) Extraction: Weigh 0.8 kg of Radix Paeoniae Alba raw material, crush the raw material and pass through a 100-mesh sieve, dissolve it in 2.4 kg of water, adjust the pH value to 6 with 1mol / L hydrochloric acid, add cellulase 5u / g, pectinase 15u / g, enzymolysis temperature 40°C, enzymolysis time 3h, then placed at room temperature, detected by high performance liquid phase (HPLC), the extract contains 0.03g of paeoniflorin;

[0026] (2) Centrifugal separation:

[0027] Put the enzymatic solution into a centrifuge for centrifugation, the centrifuge speed is 5000 rpm, after 15 minutes, collect the supernatant;

[0028] (3) Adsorption:

[0029] After the obtained supernatant was concentrated and filtered, it was applied to D101 macroporous adsorption resin, firstly eluted with water at an elution rate of 1.8mL / min, and then eluted with 70% etha...

Embodiment 2

[0035] A method for enzymatically extracting paeoniflorin from Radix Paeoniae Alba, comprising the following steps in sequence,

[0036] (1) Extraction: Weigh 1 kg of Radix Paeoniae Alba raw material, crush the raw material and pass through a 100-mesh sieve, dissolve it in 5 kg of water, adjust the pH to 5 with 1 mol / L hydrochloric acid, add 10 u / g of cellulase, 30 u / g of pectinase , the enzymolysis temperature is 45°C, the enzymolysis time is 2.5h, and then placed at room temperature, the extract contains 0.03g of paeoniflorin as detected by high performance liquid chromatography (HPLC);

[0037] (2) Centrifugal separation:

[0038] Put the enzymatic solution into a centrifuge for centrifugation, the centrifuge speed is 5000 rpm, after 15 minutes, collect the supernatant;

[0039] (3) Adsorption:

[0040] After the obtained supernatant was concentrated and filtered, it was applied to D101 macroporous adsorption resin, firstly eluted with water at an elution rate of 1.8mL / mi...

Embodiment 3

[0046] A method for enzymatically extracting paeoniflorin from Radix Paeoniae Alba, comprising the following steps in sequence,

[0047] (1) Extraction: Weigh 1.5 kg of Radix Paeoniae Alba raw material, crush the raw material and pass through a 100-mesh sieve, dissolve it in 9 kg of water, adjust the pH to 7 with 1mol / L sodium hydroxide, add 25 u / g of cellulase, pectinase 35u / g, the enzymolysis temperature is 60°C, the enzymolysis time is 2 hours, and it is placed at room temperature. After detection by high performance liquid phase (HPLC), the extract contains 0.04g of paeoniflorin;

[0048] (2) Centrifugal separation:

[0049] Put the enzymatic solution into a centrifuge for centrifugation, the centrifuge speed is 5000 rpm, after 15 minutes, collect the supernatant;

[0050] (3) Adsorption:

[0051] After the obtained supernatant was concentrated and filtered, it was loaded on D101 macroporous adsorption resin, firstly eluted with water at an elution rate of 1.8mL / min, and...

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Abstract

The invention relates to the technical field of extracting active ingredients of Chinese medicaments, in particular to a method for extracting and separating paeoniflorin from white paeony root. The method needs to solve the problems of complex process, huge consumption of organic solvents, difficult operation and low yield in the prior art. The technical scheme of the method comprises the following steps of: 1, extraction: performing two-enzyme enzymolysis of the white paeony root by cellulose-pectinase; 2, macroporous resin adsorption: collecting 70 percent ethanol elution for vacuum concentration; and 3, drying: performing vacuum drying of paeoniflorin solution subjected to vacuum concentration for 12 hours to obtain the paeoniflorin product (97 percent). The method has the advantages of simple process, short flow, low operational temperature, little loss of active ingredients, easy operation, low cost, high yield, high purity and easy realization of industrialized production.

Description

technical field [0001] The invention relates to the technical field of extraction of effective components of traditional Chinese medicines, in particular to a method for enzymatically extracting paeoniflorin from Radix Paeoniae Alba. Background technique [0002] Radix Paeoniae Alba is the dried root of Paeonia lactiflora Pall, a plant of the family Ranunculaceae, and its main active ingredient is paeoniflorin. The 2000 edition of "Chinese Pharmacopoeia" records that Radix Paeoniae Alba has the effects of nourishing blood and softening the liver, relieving pain in the middle, and astringing yin and sweating. It is one of the main ingredients in the classic famous prescription Siwu Decoction (rehmannia glutinosa, angelica, peony root, and chuanxiong) in the Song Dynasty. The main active ingredients of peony root are paeoniflorin, albiforin, and hydroxypaeoniflorin. (oxypaeoniflorin), benzoylpaeoniflorin (benzoylpaeoniflorin) and benzoylhydroxypaeoniflorin (benzoyloxypaeonifl...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08
Inventor 吕欣张瑜张岗王玉堂
Owner NORTHWEST A & F UNIV
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