Rigid ceramic/agarose composite microsphere and preparation method thereof

A technology of composite microspheres and ceramic microspheres, which is applied in the direction of microsphere preparation, microcapsule preparations, chemical instruments and methods, etc., can solve the problems of difficult to obtain products with high porosity, difficult control of foaming process conditions, and uneven distribution of pores. Uniformity and other issues to achieve the effect of stable structure, easy control and amplification, and low cost

Inactive Publication Date: 2010-11-10
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The traditional method of adding a pore-forming agent has uneven distribution of pores and it is difficult to obtain products with high porosity; the process conditions of the foaming method are difficult

Method used

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  • Rigid ceramic/agarose composite microsphere and preparation method thereof
  • Rigid ceramic/agarose composite microsphere and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Mix 12 g of nano-silica, 2 g of citric acid, and 30 mL of water, add 20 mL of 5% sodium alginate solution and continue to stir evenly to a gel. Transfer the above ceramic slurry to a 500mL three-necked flask, add 200mL pump oil, 10mL Span 80, stir for 30min at 600rpm, add 10mL saturated calcium chloride dropwise, and continue stirring for 20min. After the reaction, the microspheres are collected by filtration, cleaned with alcohol and deionized water, dried in an oven at 60°C, heated in a muffle furnace to 1200°C, kept for 30 minutes, and cooled naturally to obtain porous ceramic microspheres. The ceramic microspheres were immersed in a 4% agarose solution, incubated at 105° C. for 30 minutes, cooled naturally, and ground with 25 mL deionized water. The water was sieved to remove the surface agarose, and the microspheres were washed with deionized water three times. The obtained rigid ceramic / agarose composite microspheres have a true wet density of 1.3 g / mL, the volume ...

Embodiment 2

[0026] Mix 12 g of nano-silica, 2 g of citric acid, and 30 mL of water, add 20 mL of 5% sodium alginate solution and continue to stir evenly to a gel. Transfer the above ceramic slurry to a 500mL three-necked flask, add 200mL pump oil, 10mL Span 80, stir for 30min at 800rpm, add 10mL saturated calcium chloride dropwise, and continue stirring for 20min. After the reaction, the microspheres are collected by filtration, cleaned with alcohol and deionized water, dried in an oven at 60°C, heated in a muffle furnace to 1200°C, kept for 30 minutes, and cooled naturally to obtain porous ceramic microspheres. The ceramic microspheres were immersed in a 4% agarose solution, incubated at 105° C. for 30 minutes, cooled naturally, and ground with 25 mL deionized water. The water was sieved to remove the surface agarose, and the microspheres were washed with deionized water three times. The obtained rigid ceramic / agarose composite microspheres have a true wet density of 1.3 g / mL, the volume ...

Embodiment 3

[0028] Mix 14 g of nano-silica, 3 g of citric acid, and 30 mL of water, add 20 mL of 5% sodium alginate solution and continue to stir evenly to a gel. Transfer the above ceramic slurry to a 500mL three-necked flask, add 200mL pump oil, 10mL Span 80, stir at 600rpm for 30min, add 10mL saturated calcium chloride dropwise, and continue stirring for 20min. After the reaction, the microspheres are collected by filtration, cleaned with alcohol and deionized water, dried in an oven at 60°C, heated in a muffle furnace to 1200°C, kept for 30 minutes, and cooled naturally to obtain porous ceramic microspheres. The ceramic microspheres were immersed in a 6% agarose solution, incubated at 105°C for 30 minutes, and naturally cooled with 25 mL of deionized water for full grinding, the water was sieved to remove the surface agarose, and the microspheres were washed with deionized water 3 times. The obtained rigid ceramic / agarose composite microspheres have a true wet density of 1.5 g / mL, the ...

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Abstract

The invention discloses a rigid ceramic/agarose composite microsphere and a preparation method thereof. The composite microsphere has a uniform composite structure of a rigid ceramic skeleton and agarose gel. The preparation method comprises the following steps of: firstly, preparing ceramic slurries, and uniformly mixing nano-silica powder, zirconia powder or titanium dioxide powder with sodium alginate solution; secondly, preparing a porous ceramic microsphere, inverse suspension dispersing the ceramic slurries in an oil phase, dropping calcium chloride solution to for curing into microspheres, collecting the microspheres, drying, forming the microspheres to through high temperature sintering; thirdly, filling agarose, immersing the ceramic microspheres into agarose solution, and cooling and solidifying after preserving the temperature in a sterilizing pot for a period of time; and fourthly, grinding and screening: grinding a solide mixture of ceramic and the agarose in a mortar, obtaining the microspheres with the required particle size through screening in water, and removing the agarose on the surfaces of the microspheres. The microsphere has the advantages of good rigidity, good controllable density, good hydrophilicity, good sphericity and low cost. Shown by test experiments, the microsphere can be used as a matrix of biomacromolecule chromatographic separation medium.

Description

Technical field [0001] The invention relates to a rigid ceramic / agarose composite microsphere and a preparation method thereof Background technique [0002] Chromatographic separation technology has the advantages of high resolution, simple equipment, mild operating conditions, and is conducive to maintaining the activity of biological macromolecules. It is one of the most effective and commonly used biological separation technologies and methods, and the key to chromatographic technology It is the chromatography medium. As the demand for biological products continues to grow, the requirements for chromatography media are getting higher and higher. The ideal chromatography medium should have excellent hydrophilicity, mechanical strength, chemical stability, chemical modifiable and macroporous characteristics. [0003] Polysaccharide soft gums (such as cellulose, dextran, agarose, etc.) have good hydrophilicity and good biocompatibility with proteins. They can be easily chemically...

Claims

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Application Information

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IPC IPC(8): B01J13/04B01J20/24B01J20/281B01J20/30
Inventor 夏海锋沈建华金雄华徐斌饶志明
Owner JIANGNAN UNIV
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