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Method for constructing composite bacteria system for decaying maize straws

A technology of corn stalks and construction methods, applied in the field of microbial screening, can solve the problems that can only be solved at normal temperature and high temperature, and achieve the effect of accelerating decomposition and good decomposition effect

Inactive Publication Date: 2010-11-17
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to solve the defect that the direct return of straw to the field in the prior art can only have a good ability to degrade straw at room temperature and high temperature, and under the condition of liquid fermentation, and to invent a compound decomposing corn straw Strain construction method

Method used

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  • Method for constructing composite bacteria system for decaying maize straws
  • Method for constructing composite bacteria system for decaying maize straws
  • Method for constructing composite bacteria system for decaying maize straws

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0026] A method for constructing a composite bacterial line for decomposing corn stalks, comprising the following steps:

[0027] (1) Enrichment culture of flora: Take the soil that corn stalks have been returning to the field for many years and place it on the bottom layer of a 32L plastic bucket with a thickness of 15cm, spread 5cm thick fresh corn stalks in the middle, and then spread 15cm thick on it In the soil, adjust the C / N value to 30, cultivate at room temperature for 2 months, and regularly spray distilled water to prevent the surface from being too dry;

[0028] (2), preparation of solid medium: crush corn stalks through a 1 mm sieve, weigh 100 g of corn stalk powder, add 30 g of starch-free bran, adjust the C / N value to 28, add sterilized at 121 ° C for 30 minutes Inorganic salt basic nutrient salt solution, the water content of the mixture is 40%, two pieces of Xinhua No. 1 filter paper are placed on the surface of the culture medium, and cultured at 30°C; the in...

Embodiment 2

[0033] This example is exactly the same as example 1, the only difference is that in step (1) the C / N value is adjusted to 30 by adding urea; the subculture in step (5) refers to the cultivation of the complex bacterial strain for more than 5 generations .

Embodiment 3

[0035] This example is exactly the same as Example 2, except that the inorganic salt basic nutrient salt solution in step (2) is sterilized at 121° C. for 30 minutes.

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Abstract

The invention relates to a method for constructing a composite bacteria system for decaying maize straws, which belongs to the technical field of a microorganism screening method. The method for constructing the composite bacteria system for decaying the maize straws comprises the following steps of: (1) performing enrichment culture on bacteria; (2) preparing a solid culture medium; (3) performing inducing culture of nutrient limitation; (4) performing inducing culture of gradient temperature reduction; and (5) performing successive transfer culture to obtain the composite bacteria system CSS-1 for decaying the maize straws. The composite bacteria system screened by the method comprises bacteria, fungus and actinomycetes. The method has the advantages that: the composite bacteria system which comprises the bacteria, the fungus and the actinomycetes is obtained by screening under the condition of the solid culture medium; and the decaying effect is good.

Description

technical field [0001] The invention belongs to the technical field of microbial screening methods, and in particular relates to a method for constructing a composite bacterial system for decomposing corn stalks. Background technique [0002] Straw accounts for more than 50% of crop production. It is an extremely abundant and directly usable renewable organic resource. It is also the fourth largest energy source in the world after coal, oil and natural gas. The annual amount of crop straw resources in my country is nearly 700 million tons, accounting for almost 1 / 3 of the world's total straw resources. An average of 35.3% of straw is burned in the open air in various regions of my country every year. A large amount of straw is wasted or burned on the spot, which not only causes Serious environmental pollution and fire hazards, but also caused a huge waste of resources. [0003] Straw itself is a good biomass energy. The organic components in straw are mainly cellulose and h...

Claims

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Application Information

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IPC IPC(8): C12N1/00C05F11/08
Inventor 李俊李力沈德龙关大伟陈慧君姜昕杜秉海刘尧曹凤明冯瑞华杨小红
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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