Method for supercritical carbon dioxide extraction of lycopene in watermelon

A technology of lycopene and carbon dioxide, which is applied in chemical instruments and methods, extraction and purification/separation, organic chemistry, etc., can solve the problems of difficult implementation, high equipment requirements, and poor extraction effect.

Inactive Publication Date: 2010-11-24
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The lycopene extracted by supercritical carbon dioxide has no peculiar smell and no solvent residue, but the yield of lycopene has always been low
Chinese patent CN1334328A introduces the method of using supercritical extraction of lycopene, because no entrainer is used, and the molecular weight of lycopene is greater than 500, the solubility of lycopene is not large under supercritical liquid carbon dioxide, so its extraction effect is not good
Chinese patent CN1364832A introduces the method of using carbon dioxide supercritical extraction of lycopene in tomato, the entrainer used is a mixture of ethanol and acetone, the use of acetone does not meet the needs of green environmental protection
Chinese patent CN1799674A introduces the method of using supercritical carbon dioxide to extract lycopene from tomato waste, but it is difficult to implement due to high pressure, high energy consumption and high equipment requirements
There is no patent disclosure of supercritical fluid extraction of watermelon lycopene

Method used

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  • Method for supercritical carbon dioxide extraction of lycopene in watermelon
  • Method for supercritical carbon dioxide extraction of lycopene in watermelon
  • Method for supercritical carbon dioxide extraction of lycopene in watermelon

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Embodiment 1

[0033] The invention mainly includes three steps of watermelon pretreatment, supercritical extraction, and material collection to complete the extraction of lycopene in the watermelon.

[0034] Watermelon pretreatment:

[0035] After removing the seeds from the melon pulp, use a high-speed centrifuge at a speed of 3000-5000 rpm for 2-10 minutes to separate the melon pulp and supernatant. Dry the red precipitate of melon pulp after high-speed centrifugation. The drying process can be hot air drying at a temperature lower than 50°C; or vacuum freeze drying. The water content of the dried melon pulp solids is controlled at less than 10%. After the pulp solids are crushed, the particles are screened, and the supercritical extraction is carried out with a particle size of less than 40 mesh.

[0036] Carbon dioxide supercritical extraction of watermelon lycopene:

[0037] Weigh 2 grams of solid melon pulp pulverized particles less than 40 mesh, 0.4 mg of vitamin C, 5 microliters...

Embodiment 2

[0041] Experimental operation procedure and step are by embodiment 1, and concrete data sees the following table:

[0042]

[0043] Detection method and detection index:

[0044] The method for extracting lycopene with supercritical carbon dioxide in the present invention is suitable for extracting lycopene in plant fruits, especially for extracting lycopene in watermelon.

[0045] The determination of lycopene content applicable to this extraction method in the present invention can use high-performance liquid chromatography, and can also use a spectrophotometer. Lycopene is a fat-soluble unsaturated hydrocarbon with the molecular formula C 40 h 56 , with a molecular weight of 539.6, usually dark red powder or oily liquid, the pure product is needle-like dark red crystals, and its English name is Lycopene. Lycopene has a strong absorption peak at 472 nm, and 503 nm is its characteristic absorption. Using the characteristic absorption of lycopene, the rapid determinatio...

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Abstract

The invention discloses a method for supercritical carbon dioxide fluid extraction of lycopene in watermelons, comprising the following steps: pretreating the watermelons, carrying out supercritical carbon dioxide extraction with proper granularity at the pressure of 210-270bar and temperature of 60-80 DEG C for 100-150 minutes, wherein ethanol is used as an entrainer, the amount of the ethanol is 30-40% of the volume of an extraction kettle, and the charging amount of watermelon solid powder is 10-20% of the volume of the supercritical carbon dioxide extraction kettle; wherein vitamin C and polyglycerol fatty acid ester are used as addictives during the supercritical extraction process, and then repeatedly collecting materials after extraction is completed. The method for supercritical carbon dioxide fluid extraction of the lycopene in the watermelons can help improve the extraction yield of the lycopene up to 37%, and the extraction product has low solvent content and less environmental pollution in production process.

Description

technical field [0001] The invention relates to an extraction of watermelon lycopene, namely, a method for extracting lycopene in watermelon by using carbon dioxide as a supercritical fluid. Background technique [0002] Red watermelon is rich in lycopene and is the main source of natural lycopene. The latest research found that the average content of lycopene in high-quality red watermelon fruit is 4.87 mg / 100 g fresh weight, while the average content of lycopene in fresh tomato fruit is 3.10 mg / 100 g fresh weight. The lycopene contained in tomato is more than 40% higher than that contained in tomatoes of equal weight. Studies have shown that lycopene in tomatoes can be absorbed more effectively by the human body only after being heated or processed, while lycopene in watermelon can be directly absorbed by the human body without heating or processing. [0003] The research results show that the molecular structure of lycopene is a long chain with 11 conjugated double bond...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C11/21C07C7/10C09B61/00C09B67/10
CPCY02P20/54
Inventor 赵文恩刘伟胡利强赵蕾李雯
Owner ZHENGZHOU UNIV
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