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Medical applications and preparation methods of Pleurotus ferulae Lanzi polysaccharide and composites thereof

A technology of Pleurotus ferulae and polysaccharide, which is applied in the field of Pleurotus ferulae polysaccharide and pharmaceutical compositions containing Pleurotus ferulae polysaccharide, and can solve the problems of weakening anti-tumor immune response and the like

Active Publication Date: 2010-12-15
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, various treatments can attenuate the antitumor immune response

Method used

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  • Medical applications and preparation methods of Pleurotus ferulae Lanzi polysaccharide and composites thereof
  • Medical applications and preparation methods of Pleurotus ferulae Lanzi polysaccharide and composites thereof
  • Medical applications and preparation methods of Pleurotus ferulae Lanzi polysaccharide and composites thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0113] Example 1: Extraction and preparation of Pleurotus ferulai polysaccharide

[0114] Pleurotus ferulai (fresh) 20kg, cut into small pieces, add 400ml of water per 100g, squeeze the juice with a juicer for 1min, ultrasound for 30min, boil for 3h, cool, centrifuge to obtain the supernatant, filter paper suction. Precipitate and add 6 to be measured water to ultrasonic for 30min, boil for 1h, cool, centrifuge, take the supernatant, filter with filter paper, combine the supernatants and concentrate to obtain 9L of concentrate. The yield rate is 7.825%.

Embodiment 2

[0115] Example 2: Purification of Pleurotus ferulai polysaccharide

[0116] 50ml of Pleurotus ferulai concentrate prepared in Example 1 (equivalent to 50mg of raw material) was added with 0.6g of papain, adjusted to PH5-6, 60°C water bath for 1.5h, placed at room temperature, centrifuged at 3000r / min for 5min, took the supernatant and added Ethanol to 85% of the alcohol content, shake and place, centrifuge at 3000r / min for 5min, take the precipitate, repeat the operation 3 times, and obtain the purified Pleurotus ferulai polysaccharide.

Embodiment 3

[0117] Example 3: Investigation of papain enzymatic hydrolysis process

[0118] 1. Determination of the most suitable enzyme dosage:

[0119] Take 7 conical flasks with stoppers, add 20ml (43.5mg / ml) of Pleurotus ferulae extract to each flask, add papain (mass ratio of enzyme to substrate) 0mg / g, 2mg / g, 4mg / g, 6mg / g, 8mg / g, 10mg / g, 12mg / g, adjust PH6 with HCL, 50℃ water bath for 2h and then boiling water bath for 10min to inactivate the enzyme, cool to room temperature, centrifuge at 3000r / min for 5min, take the supernatant and add 95% Ethanol to alcohol concentration 80%, 3000r / min centrifugation for 10min to take the precipitate, wash the precipitate three times in the same way, and measure the yield of polysaccharide. The best enzyme base ratio is: 8mg / g.

[0120] Enzyme base ratio mg / g

[0121] 2. Determination of the optimal enzymolysis time:

[0122] Take 7 conical flasks with stoppers, add 20ml (43.5mg / ml) of Pleurotus ferulae extract to each bottle, add 6.96mg of papain ...

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PUM

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Abstract

The invention relates to medical applications and preparation methods of Pleurotus ferulae Lanzi polysaccharide and composites thereof. The preparation methods of the invention comprise the following steps of extracting, removing protein and purifying, wherein the step of removing protein adopts the following methods of: (1) a solvent extraction method; (2) an ion exchange resin method; (3) an enzyme method; and (4) a gel chromatography; and the step of purifying adopts the following methods of: (1) the solvent extraction method; (2) an ethanol precipitation method; (3) a liquid-liquid counter-current distribution chromatography; and (4) the gel chromatography.

Description

[0001] Technical Field: The present invention relates to Pleurotus ferulae polysaccharides prepared from Pleurotus ferulae Lanzi and pharmaceutical compositions containing Pleurotus ferulae polysaccharides, as well as their preparation, medical use and analysis methods. Background technique: [0002] Cervical cancer is one of the most common gynecological malignancies. Xinjiang is a high-incidence area of ​​cervical cancer in my country. The morbidity and mortality rates are both high, and there is a trend of increasing year by year and younger. The treatment of cervical cancer has developed from traditional local surgery and radiotherapy to systemic treatment-chemotherapy. However, a variety of treatment methods can weaken the anti-tumor immune response. Therefore, in the treatment of cervical cancer, improving the body's immune function plays an important role in the process of eliminating tumor cells. [0003] At present, a large number of clinical studies have confirmed that C...

Claims

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Application Information

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IPC IPC(8): C08B37/00C12S3/02A61K31/715A61K47/46A61K47/26A61P35/00A61P37/04A61P7/02A61P1/00A61P39/00A61P25/28A61P9/10A61P17/16A61P9/00A61P3/06A61P39/06A23L1/09G01N21/78G01N30/02A23L33/125
Inventor 王金辉李国玉巩平魏秀岩黄健王莹杨琳卢立娜秦颖张翠
Owner SHIHEZI UNIVERSITY
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