Preparation method of multifunctional nanometer composite particle constructed by modified polymine

A nano-composite particle, polyethyleneimine technology, applied in biochemical equipment and methods, chemical instruments and methods, preparations for in vivo tests, etc., can solve the problem of low fluorescence yield, uneven crystal particle size, biological phase Capacitance needs to be improved urgently

Active Publication Date: 2010-12-29
SICHUAN UNIV
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Problems solved by technology

The nanocrystals prepared by the aqueous phase synthesis method can be directly dispersed in water, but due to the inhomogeneous particle size and wide particle size distribution of the synthesized crystals, the physical properties are lacking compared with the crystals prepared by the organic phase. The yield is lower than that of quantum dots prepared in the organic phase, and the magnetic properties of superparamagnetic iron oxide crystals synthesized in the aqueous phase are also poor
This leads to limitations in the biological and industrial applications of aqueous-phase synthesized nanocrystals.
High-performance nanocrystals prepared from organic phases have uniform particle size distribution and controllable physical properties, but biocompatibility needs to be improved urgently

Method used

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  • Preparation method of multifunctional nanometer composite particle constructed by modified polymine
  • Preparation method of multifunctional nanometer composite particle constructed by modified polymine
  • Preparation method of multifunctional nanometer composite particle constructed by modified polymine

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Effect test

Embodiment 1

[0066] Concentration of 10mg / ml, monodisperse Fe in n-hexane 3 o 4 Add the nanocrystals into the reagent bottle, remove the organic solvent by vacuum drying, and then weigh the Fe 3 o 4 0.001g of nanocrystals; 10g of a chloroform solution of saturated dodecane-modified branched polyethyleneimine with a concentration of 20mg / ml and the above-mentioned dried nanocrystals were shaken and mixed evenly at a temperature of 25°C; the above-mentioned organic solution was mixed at a temperature of 25°C ℃, ultrasonic power 130W, amplitude 65%, added to 100g of pure water, oscillated and shaken for 24 hours; the residual trichloromethane solvent in the above aqueous phase solution was removed by vacuum rotary evaporation method; modified polyethyleneimine was obtained Pack Fe 3 o 4 Nanocomposite particles constructed from nanocrystals. Such as figure 2 As shown, the results of transmission electron microscopy show that the modified polyethyleneimine wraps Fe 3 o 4 The nanocomposi...

Embodiment 2

[0068] The concentration is 8mg / ml, monodisperse MnFe in n-hexane 2 o 4 Add the nanocrystals into the reagent bottle, remove the organic solvent by vacuum drying, and then weigh the MnFe 2 o 4 0.5 g of nanocrystals; 10 g of a tetrahydrofuran solution of polycaprolactone-modified branched polyethyleneimine with a concentration of 20 mg / ml and the above-mentioned dry nanocrystals were shaken and mixed evenly at a temperature of 25 ° C; the above-mentioned organic solution was mixed at a temperature of 30 ° C, Ultrasonic power 130W, amplitude 65%, was added to 5g of pure water, oscillated and shaken for 24 hours; the residual tetrahydrofuran solvent in the above aqueous phase solution was removed by vacuum rotary evaporation method; modified polyethyleneimine-coated MnFe 2 o 4 Nanocomposite particles constructed from nanocrystals. Such as Figure 4 As shown, the results of transmission electron microscopy show that the modified polyethyleneimine wraps MnFe 2 o 4 Nanocompos...

Embodiment 3

[0070] Add FePt nanocrystals with a concentration of 2mg / ml and monodisperse in n-hexane into the reagent bottle, remove the organic solvent by vacuum drying, then weigh 0.001g of FePt nanocrystals; 1 g of chloroform solution of ethyleneimine and the above-mentioned dry nanocrystals were shaken and mixed evenly at a temperature of 25 ° C; Shake the solution for 24 hours; remove the residual chloroform solvent in the aqueous phase solution by vacuum rotary evaporation; prepare nanocomposite particles constructed of modified polyethyleneimine wrapped FePt nanocrystals.

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Abstract

The invention discloses a preparation method of a multifunctional nanometer composite particle constructed by modified polymine, which is characterized by converting the organic phase of a nanometer crystalloid synthesized by an organic phase to a water phase. In the phase conversion process, the agglomeration of a large quantity of particles are not required to occur so as not to cause precipitation in a pure water solvent or a buffer water solvent, and the controllability of dispersion degree needs to be realized according to different uses; the nanometer particles subjected to phase conversion are decorated by adding different surface groups according to application conditions to meet the necessary function and characteristic requirement; and the nanometer crystalloid is covered by themodified polymine to keep the original physical characteristic and have the excellent dispersion performance in the water phase. The nanometer composite particle can carry hydrophobic nanometer crystalloid and medicines to be applied to molecular imaging and medicine delivery, and can be used as a gene carrier to be applied to gene transfection; meanwhile, gold ion or silver ion in the water solution is reduced by utilizing amino on the modified polymine in the nanometer composite particle to realize in-situ crystalloid growth so as to construct a multifunctional molecular developing probe.

Description

technical field [0001] The invention relates to a preparation method of multifunctional nanocomposite particles constructed by modified polyethyleneimine, and belongs to the fields of biomedical materials, drug delivery and molecular imaging. Background technique [0002] At present, multifunctional nanoparticles (such as light-responsive nanoparticles, magnetic-responsive nanoparticles, and electrical-responsive nanoparticles) have been widely used in biological fields such as molecular imaging and cell sorting. For example, superparamagnetic iron oxide nanoparticles have been used as magnetic resonance imaging agents (such as the existing product Feridex I.V.) and drug carriers (Langmuir 2007, 23, 6342); photoluminescent quantum dots have been fully used in cell imaging. Confirmed and affirmed (Nature Biotechnology 2004, 22, 8), and according to the latest scientific research, gold nanoparticles are expected to be used as a new generation of X-ray imaging agents (The Briti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L79/02C08K3/22C08K3/08C08K3/30A61K47/32A61K49/12C07K5/12C07K7/08C12N5/00C12N15/63
Inventor 艾华王志勇刘刚刘琛苏红莹马娜
Owner SICHUAN UNIV
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