Kit and method for extracting microbial DNA

Abstract

The invention relates to the field of molecular biology and discloses a kit and a method for extracting microbial DNA. The kit comprises lysis solution, inhibitor removal solution and binding solution, wherein the lysis solution comprises 50 to 200mM of Tris-HCl, 50 to 150mM of EDTA, 0.5 to 3M of NaCl, 0.5 to 2 percent of CTAB, 0.5 to 2 percent of PVP and 0.5 to 2 percent of SDS; the inhibitor removal solution comprises 100 to 300mM potassium acetate, sodium acetate or ammonium acetate and 50 to 200mM aluminum sulfate, ammonium sulfate or aluminum ammonium sulfate; and the binding solution comprises 3 to 6M guanidine hydrochloride, 10 to 50mM Tris-HCl and 5 to 50 percent isopropanol. The kit and the method for extracting the microbial DNA have the advantages of high purity, high universality, high extraction speed, direct use for a downstream experiment, and application to extracting DNA from a microbe-containing sample.

Description

technical field

[0001] The invention relates to the field of molecular biology, in particular to a kit for extracting microbial DNA, and also to a method for extracting microbial DNA. Background technique

[0002] Microorganisms are the general term for all tiny organisms that are invisible or unclear to the naked eye. They are a large group of organisms including bacteria, viruses, fungi, and some small protozoa and microscopic algae. They are small in size and simple in structure, but they are closely related to human life. They cover many beneficial and harmful species, and are widely involved in many fields such as health, food, medicine, industry, agriculture, and environmental protection.

[0003] Soil microorganisms are rich in resources and are important life forms in terrestrial ecosystems. They are very sensitive to the external environment, have poor stress resistance, and are easily affected by various adverse external environments. Therefore, soil microorganism...

Images