Novel technology for granular latex gel complex carrier immobilized cell

A granular and cell-based technology, applied in the direction of immobilization on or in the inorganic carrier, fixed on/in the organic carrier, etc., can solve the problem of affecting the strength and service life of immobilized particles, low mechanical strength of agar gel, The strength of calcium alginate is not high, and the immobilized cells have high activity, low price, and strong ability to withstand changes in hydraulic water quality.

Inactive Publication Date: 2011-01-19
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These polymers have certain disadvantages, such as: most immobilized carriers are water-soluble, which will directly affect the strength and service life of immobilized particles
The particles prepared by cross-linking polyvinyl alcohol and boric acid have water-soluble swelling properties. As time goes by, the particle volume increases, which makes the particles weak and the service life is relatively short.
Agar gel has low mechanical strength and poor permeability
Polyacrylamide gel monomer has high toxicity, low activity, and is not easy to form
The strength of calcium alginate is not high, and the preparation process of using it to immobilize cells is more complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Activate 1g of active dry yeast in 10ml of 3% glucose solution for half an hour, add 1.5g of latex and stir evenly according to the gel-bacteria ratio of 1.5:1, then add 25g of volcanic magma filter material with a particle size of 5-6mm, and stir Mix evenly, dry in a constant temperature drying oven at 40±5°C for 0.5 to 1 hour, take it out and wash it with clean water three times to make immobilized granules.

[0020] In a 300ml Erlenmeyer flask, add immobilized particles and a glucose solution with a concentration of 10% and ferment at a constant temperature at 35°C. The pH of the fermentation broth is 5.0, and the added nutrient salt: KH 2 PO 4 2.5g / L, MgSO 4 0.25g / L, CaCI 2 0.25g / L, urea 0.24g / L.

[0021] After one day, the utilization rate of reducing sugar reaches more than 90%, and the yield of ethanol is 45%. The fermentation broth is added intermittently and batches for reaction. Above 40%.

Embodiment 2

[0023] Activate 1g of active dry yeast in 10ml of 3% glucose solution for half an hour, add 1g of latex and stir evenly according to the gel-to-bacteria ratio of 1:1, then add 12g of concrete porous bricks with a particle size of 3mm, stir and mix evenly, Dry in a constant temperature drying oven at 40±5°C for 0.5 to 1 hour, take it out and wash it with clean water three times to make immobilized granules.

[0024] In a 300ml Erlenmeyer flask, add immobilized particles and a glucose solution with a concentration of 12.5% ​​and ferment at a constant temperature at 35°C. The pH of the fermentation broth is 5.0, and the added nutrient salt: KH 2 PO 4 2.5g / L, MgSO 4 0.25g / L, CaCI 2 0.25g / L, urea 0.24g / L.

[0025] After one day, the utilization rate of reducing sugar reaches more than 90%, and the yield of ethanol is 44%. The fermentation broth is added intermittently and batches for reaction. %above.

Embodiment 3

[0027] Activate 1g of active dry yeast in 10ml of 2% glucose solution for half an hour, add 1g of latex and stir evenly according to the gel-bacteria ratio of 1:1, then add 12g of concrete porous bricks with a particle size of 5-6mm, stir and mix evenly , adding a concentration of 5% CaCl 2 As a coagulant, stand for 5 minutes and rinse with water three times to make immobilized particles.

[0028] In a 300ml Erlenmeyer flask, add immobilized particles and a glucose solution with a concentration of 7% and ferment at a constant temperature at 30°C. The pH of the fermented liquid is 5.0. After one day, the utilization rate of reducing sugar reaches more than 90%, and the ethanol yield is 41%. The fermentation liquid is added intermittently in batches to react, the reaction period is one day, the utilization rate of the reducing sugar is over 90% over 60 days, and the ethanol yield is over 40%.

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PUM

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Abstract

The invention belongs to the technical field of immobilized cells, in particular to a method for using a complex carrier of latex and a porous material as an immobilized carrier. The method comprises the following steps of: taking microbes subjected to activation and proliferation, mixing the microbes and the latex, and stirring and uniformly mixing; adding base material granules and stirring and uniformly mixing; drying for 0 to 4 hours at the temperature of between 50 below zero and 80 DEG C or adding a flocculant to gelatinize the latex; and finally, taking out and cleaning by using tap water, wherein the latex is used for specific engineering demands.

Description

technical field [0001] The invention relates to the technical field of microorganism immobilization, in particular to a method for using a composite carrier of latex and a porous material as an immobilized carrier and the application of the immobilized cells in water treatment and fermentation engineering. Background technique [0002] Immobilized cell technology is a technology that combines free cells or microorganisms with water-insoluble carriers through chemical or physical methods to make them insoluble in water but still have high biological activity. Immobilized cell technology is beneficial to the screening, purification and immobilization of bacteria while immobilizing cells. Compared with the general suspension microorganism treatment method, it can eliminate the loss of microorganisms, facilitate the recycling of microorganisms, and facilitate continuous production. The production operation is convenient for the control of the treatment process, which can effecti...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/08C12N11/04
Inventor 周小凡张帅马金霞吴伟兵
Owner NANJING FORESTRY UNIV
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