Pressure pulsation ultrasonic atomization culture device and cultural method using same

A technology of ultrasonic atomization and pressure pulsation, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as inability to carry out large-scale cultivation, enlargement of reactor volume, poor dispersion of droplets, etc., and save raw materials. , High production efficiency, uniform growth state

Inactive Publication Date: 2011-01-26
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

This process improves the concentration of nutrient mist in the atomization reactor and makes the atomization liquid evenly distributed, but there is still the problem of liquid escape, which increases the probability of bacterial contamination during the liquid replenishment process
[0005] The dispersion process of the mist in the reactor mainly relies on two kinds of energy, one is the kinetic energy generated by ultrasound, the kinetic energy generated by the ultrasound makes the culture liquid form a sprayed water column to push the generated nutrient mist upward. The degree of dispersion in the reactor is very poor, and the volume of the reactor is limited, so large-scale cultivation cannot be carried out; the other is to rely on pneumatically driving the droplets to disperse the droplets in the reactor. The advantage of this method is that the droplets The dispersion in the reactor is more sufficient, and it has the potential to scale up the process, but its disadvantage is that the droplets will escape with the overflow of the air flow at the same time, causing the loss of the culture solution and increasing the chance of bacterial contamination

Method used

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  • Pressure pulsation ultrasonic atomization culture device and cultural method using same
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Examples

Experimental program
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Effect test

Embodiment 1

[0043] Rapid Propagation of Rhodiola longipsifolia by Using Pressure Pulse Ultrasonic Atomization Culture Device

[0044] Such as figure 1 As shown, the pressure pulsation ultrasonic atomization culture device is composed of two systems: one is the pressure control system. The system includes a time relay 4 , an air pump 3 , an air distribution tee 6 , a gas flow meter 1 , an air filter 5 and a solenoid valve 20 . Wherein the gas distribution tee 6 comprises three ports, two ports have the same size, and the diameter of the third port is 5% of the diameter of the first two ports. By adjusting the opening of the gas flow meter 1, the air flow rate entering the reactor is adjusted so that the pressure in the reactor reaches 1.2 atm. During operation, the air pump is controlled by a time relay. At the same time, the solenoid valve is closed under the control of the time relay, so that the air injected by the air pump can only flow through the larger end of the air distribution...

Embodiment 2

[0053] Rapid Propagation of Rhodiola grandiflora by Pressure Pulse Ultrasonic Atomization Culture Device

[0054] The specific implementation steps of the technique of carrying out the large-scale propagation of Rhodiola grandiflora using the same device as described in Example 1 are:

[0055] 1. Same as step 1 in Example 1.

[0056] 2. The seeds germinate on the culture medium and grow roots, stems, and leaves. Take the leaves and cut them into small sections of 0.5 cm with a scalpel. Prepare MS medium, add agar powder 5%, sucrose 30%, 2.5 mg / L BAP and 0.2 mg / L GA 3 , sterilized at 121°C for 17 minutes. The cut plant material is inoculated on the culture medium, and then placed in a culture room at 25±2°C, with a light intensity of 4000Lux and a photoperiod of 16 hours.

[0057] 3. After the plant material dedifferentiates and grows clustered buds, take out the clustered buds as the initial material for reactor culture. Prepare MS medium with 30% sucrose, 2.5 mg / L BAP and...

Embodiment 3

[0061] Rapid Propagation of Xinjiang Snow Lotus by Using Pressure Pulse Ultrasonic Atomization Cultivation Device

[0062] Except that the diameter of the third port of the gas distribution tee is 1% of the diameter of the first two ports, and the pressure in the reactor is controlled at 1.5 atm, all the other devices are the same as those described in Example 1. The specific implementation steps of the technique of adopting the above device to carry out the large-scale propagation of Xinjiang Snow Lotus are:

[0063] 1. Same as step 1 in Example 1.

[0064] 2. The seeds germinate on the medium and grow roots, stems, and leaves. Take the leaves and cut them into 0.5cm with a scalpel 2 small pieces. MS medium was prepared by adding 5% agar powder, 30% sucrose, 2.5 mg / L BAP and 0.5 mg / L NAA, and sterilized at 121° C. for 17 minutes. The cut plant material is inoculated on the culture medium, and then placed in a culture room at 25±2°C, with a light intensity of 4000Lux and a ...

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Abstract

The invention relates to a pressure pulsation ultrasonic atomization culture device and cultural method using the same. The culture device of the invention comprises an atomization control system, and the atomization control system comprises an atomizing reactor culture-tank, an atomizing transducing chip arranged at the bottom of the culture-tank, an atomization generating device for controlling the atomizing transducing chip and a time relay, wherein the time relay is used for controlling the atomization generating device to start the atomizing transducing chip to atomize nutrient solution; and meanwhile, the culture device of the invention also comprises a pressure control system arranged on an intake pipe of the atomization control system, and the pressure control system comprises the time relay, an air pump, a gas-distributing tee, a gas flowmeter, an air filter and an electromagnetic valve, wherein the air after passing through the gas flowmeter is pumped into the gas-distributing tee by the time relay and the control pump, then the air enters the atomizing reactor culture-tank after passing through the air filter. The device and the method of the invention have the advantages of saving materials, reagents and manpower, shortening the breeding cycles of seedlings and having high production efficiency, therefore, the invention is easy to be used in large-scale production.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a pressure pulsation ultrasonic atomization cultivation device and a cultivation method thereof. Background technique [0002] my country is rich in plant resources, especially medicinal plant resources. It is one of the countries with the largest variety of wild plants, the earliest utilization and the highest utilization rate in the world. It has extremely rich important prescription materials and valuable experience accumulated by traditional Chinese medicine. The advantages of developing herbal medicines are unmatched by other regions. However, due to the low level of modernization and internationalization of medicinal plants in my country, the competitiveness of herbal medicine products in the international market is restricted. In recent years, due to unscientific over-exploitation, some rare medicinal plant resources with limited reserves are on the verge of depletion. For ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘春朝郭斌
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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