Composition containing L. acidophilus 1.1878 and Chinese medicaments and application thereof

A technology of Lactobacillus acidophilus and composition, applied in the field of compositions containing Lactobacillus acidophilus and traditional Chinese medicine

Inactive Publication Date: 2011-01-26
杜丹
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no relevant literature and patent reports in China

Method used

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  • Composition containing L. acidophilus 1.1878 and Chinese medicaments and application thereof
  • Composition containing L. acidophilus 1.1878 and Chinese medicaments and application thereof
  • Composition containing L. acidophilus 1.1878 and Chinese medicaments and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Morphology and staining

[0022] Observe the colony morphology on the MRS plate with naked eyes; take a smear of a single colony, stain with Gram, and observe under a microscope. Results: The colony was colorless, with a diameter of 0.5-2.0 mm; the microscopic examination showed that the Gram stain was positive, without bacillus, and arranged in short chains. According to culture and morphological staining, it can be preliminarily determined as Lactobacillus.

Embodiment 2

[0023] Embodiment 2: biochemical reaction

[0024] a. prepare sugar-free MRS medium;

[0025] b. After filtering lactose, galactose, sucrose, mannose, mannitol, sorbitol, maltose, trehalose, rhamnose, and glucose with a 0.22 μm microporous membrane, add them to the above sugar-free In the MRS medium, an improved MRS medium is made;

[0026] c. Add 0.5ml of bromocresol violet to every 10mL of the improved medium as an indicator;

[0027] d. Take the L.acidophilus logarithmic growth phase culture solution, inoculate it in each of the above-mentioned mediums, culture it anaerobically at 37°C for 36 hours, and observe the fermentation situation.

[0028] Judgment of the results: the indicator changes from purple to yellow for positive, and green for weak positive.

[0029] Biochemical reaction result:

[0030] Table 1 L.acidophilus biochemical reaction results

[0031]

Embodiment 3

[0032] Embodiment 3: Lactobacillus acidophilus (L acidophilus 1.1878) acid resistance performance evaluation

[0033] Centrifuge the logarithmic growth phase culture solution of Lactophilus 1.1878 at 2500r / min for 5min, discard the supernatant, wash the thalline 3 times with sterile PBS buffer, adjust the bacterial concentration to 10 8 CFU / mL, the strain was inoculated in artificial gastric juice, with HCl and 0.1mol·L -1 Adjust the pH to 2.0, 3.0, and 4.0 with NaOH, and incubate in an anaerobic incubator at 37°C. At the 60th, 90th, and 180th minute, respectively, take 1 mL of the bacterial suspension and count the viable bacteria. The bacterial suspension with a pH of 6.2 was used as 0min normal control, calculate the survival rate of strains under each pH condition. The concentration of 0min is: bacterial suspension concentration × 1 / 20 (ml).

[0034]Results: As can be seen from Table 2, when the pH was 2.0, the number of live bacteria of Lactophilus (L acidophilus 1.1878...

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Abstract

The invention relates to a composition containing L. acidophilus 1.1878 and Chinese medicaments and application thereof. The L. acidophilus 1.1878 has high acid resistance and bile salt resistance and good adhesion. The composition contains the L. acidophilus 1.1878, and also contains oligosaccharide, Chinese medicinal herbs and the like. The composition can be used as food, health-care products or medicaments, is suitable for eating for a long term, and is mainly used for preventing and treating antibiotic associated diarrhea, regulating intestinal flora, protecting intestinal mucosa and enhancing immunity.

Description

(1) Technical field [0001] The invention relates to a composition containing Lactobacillus acidophilus and traditional Chinese medicine. The composition can be used as a health product or medicine, is suitable for long-term consumption, and is mainly used for preventing and treating antibiotic-associated diarrhea, regulating intestinal flora, protecting intestinal mucosa and enhancing immunity. (2) Background technology [0002] Antibiotic-associated diarrhea (AAD) is the most common adverse reaction to antibiotic therapy. Antibiotic-associated diarrhea occurs in 5% to 25% of patients receiving antibiotic therapy. With the increasing use of antibiotics, AAD cases are increasing year by year. This kind of enterogenous infection is not only difficult to cure with conventional antibiotic treatment, but also may lead to further flora imbalance, leading to the deterioration and spread of the disease, and severe cases may develop bacteremia and even death. The pathogenesis of A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/734A61K35/74A23L1/28A23L1/29A61P1/00A61P1/12A61K31/702A23L33/135A61K35/747
Inventor 杜丹王晓东张彦
Owner 杜丹
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