Large-headed atractylodes rhizome polysaccharide and preparation method and application thereof

A technology of Atractylodes Rhizoma Atractylodes Polysaccharide and Atractylodes Rhizoma Atractylodes Rhizome, which is applied in the field of preparation of Atractylodes Rhizoma Atractylodes Polysaccharides, can solve the problems of no probiotics and intestinal flora, and achieve reliable results, high reliability, and high sensitivity

Active Publication Date: 2011-01-26
KUIMING JIAJIANING BIOLOGICAL PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Atractylodes polysaccharides are also limited to good effects on immune regulation, anti-tumor, and lo

Method used

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  • Large-headed atractylodes rhizome polysaccharide and preparation method and application thereof
  • Large-headed atractylodes rhizome polysaccharide and preparation method and application thereof
  • Large-headed atractylodes rhizome polysaccharide and preparation method and application thereof

Examples

Experimental program
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Embodiment 1

[0033] Example 1. Extraction method of Baizhu polysaccharide

[0034] 1. Extraction method of Baizhu polysaccharide

[0035] Take 20g Atractylodes macrocephala (purchased from Beijing Tongrentang Baiwangshan Pharmacy), add 200mL distilled water and soak for 24h, then directly decoct the soaking liquid for 30min and then filter, add the same amount of distilled water (200mL) and decoct for another 30min, and the combined filtrate is evaporated and concentrated to 100mL , Get the extract of Atractylodes macrocephala.

[0036] 2) Extraction and purification of Atractylodes macrocephala polysaccharide

[0037] Preparation of Atractylodes macrocephala crude polysaccharide by water extraction and alcohol precipitation: After concentrating the extract of Atractylodes macrocephala, add 3 times the volume of ethanol solution (the volume percentage is 95%, and the final volume percentage of ethanol is 71.25%), and place it in a refrigerator at 4℃ for alcohol precipitation Centrifuge at 8000rpm...

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Abstract

The invention discloses a method for preparing large-headed atractylodes rhizome polysaccharide and application. The method for preparing the large-headed atractylodes rhizome polysaccharide comprises the following steps of: 1) soaking large-headed atractylodes rhizomes into water, then decocting the rhizomes, collecting filtrate, and concentrating the filtrate to obtain large-headed atractylodesrhizome extract; 2) adding ethanol into the extract, standing the extract at the temperature of 4 DEG C, centrifuging the extract and collecting sediment, dissolving the sediment, removing the ethanol, small molecular sugar, protein and salt by dialysis, and drying the extract to obtain coarse large-headed atractylodes rhizome polysaccharide; and 3) dissolving the large-headed atractylodes rhizome polysaccharide, then adding Saveg liquid in the volume which is 2 times that of the solution, mixing and shaking the solution uniformly, centrifuging the solution and collecting supernate, then removing chloroform and n-butyl alcohol by dialysis from water, and drying the supernate to obtain the large-headed atractylodes rhizome polysaccharide without containing the protein. Proved by experiments, the large-headed atractylodes rhizome polysaccharide can promote proliferation of probiotics of infants, youth, animal bifidobacterium, plant lactobacillus and the like in vitro, can be used as probiotics for production, has the effect of regulating intestinal flora balance, and can be used as novel functional probiotics for development.

Description

Technical field [0001] The invention relates to a preparation method of Atractylodes macrocephala polysaccharide and its application in regulating the balance of intestinal flora. Background technique [0002] There is often a layer of microorganisms or microbial layers in the human digestive tract, which are not only harmless to the host, but also beneficial and necessary. This microbial layer is called normal microbiota or normal bacteria flora. These microbial groups (tens of billions, accounting for 1 / 50-1 / 60 of the human body's weight) carry out active growth and metabolism activities in the human intestine, and have extremely important physiological functions. It is closely related to human digestion, absorption, immunity, anti-tumor, anti-aging, and resistance to invasion by foreign flora. Numerous research results show that age, race, dietary structure, living habits, environment, disease, antibiotic treatment, etc. will affect the human intestinal microecosystem and its...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P1/00
Inventor 李平兰刘丽莎王锐尚楠王洋
Owner KUIMING JIAJIANING BIOLOGICAL PROD CO LTD
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