High-efficiency denitrification mixed bacterial agent and application thereof
A mixed bacterial agent and denitrification technology, which is applied in the direction of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of secondary pollution, residual water, etc., to reduce chlorine consumption, improve efficiency, and improve nitrification efficiency effect
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Embodiment 1
[0038] The present invention denitrification bacteria culture process:
[0039] The ratio of medium is: 2% glucose (w / v), 1.5% beef extract (w / v), 1.5% peptone (w / v), 0.058% magnesium sulfate (w / v), 0.025% manganese sulfate (w / v), 0.22% sodium acetate (w / v), 0.2% dipotassium hydrogen phosphate (w / v), the solvent is water. The above percentages are percentages by mass and volume, and 1% concentration of a certain component means that 100 mL of culture medium contains 1 g of the component.
[0040] Fermentation conditions: the initial pH value was 5.8, CCTCC No: M 2010001 and CCTCC No: M 2010002 were inoculated respectively, and 2% glucose (w / v) and 0.22% sodium succinate (w / v) were maintained during the fermentation process. At 30°C, the stirring speed is 400r / min, the ventilation rate is 1vvm (7L tank), and the number of bacteria in the fermentation broth can reach 1.17×10 10 cfu / mL.
[0041] Sludge domestication process:
[0042] After the microbial fermentation liquid o...
Embodiment 2
[0044] The domestic sewage of Chongzhao Village Ecological Demonstration Village, Huzhou City, the raw water quality is BOD5 (Biology Oxygen Demand) 250mg / L, COD (chemical oxygen demand, chemical oxygen demand) 400mg / L, total nitrogen 30mg / L, Ammonia nitrogen 10mg / L each gets 1000ml, is placed in the glass container, carries out the aerobic stage treatment with the sludge of this village sewage treatment station and the sludge domesticated by the method of embodiment 1 respectively, and the aeration rate is 1vvm. After 18 hours of reaction, the water quality of the water sample treated with the sludge of the original sewage station was BOD5 20mg / L, COD 40mg / L, total nitrogen 20mg / L, and ammonia nitrogen 6mg / L; the water quality of the water sample treated with the domesticated sludge of this patent was BOD5 6mg / L L, COD 20mg / L, total nitrogen 7mg / L, ammonia nitrogen 3mg / L; using the mixed bacterial agent of the present invention, the overall efficiency is increased by about 50%...
Embodiment 3
[0046] Under special circumstances, the raw water quality of domestic sewage in Chongzhao Village Ecological Demonstration Village, Huzhou City is BOD5550mg / L, COD 1000mg / L, total nitrogen 60mg / L, and ammonia nitrogen 25mg / L. The sludge from the village sewage treatment station and the sludge domesticated according to the method of Example 1 were treated in an aerobic stage, and the aeration rate was 1vvm. After 24 hours of reaction, the water quality of the water sample treated with the sludge of the original sewage station was BOD5 50mg / L, COD 100mg / L, total nitrogen 30mg / L, and ammonia nitrogen 15mg / L; the water quality of the water sample treated with the domesticated sludge of this patent was BOD5 8mg / L L, COD 30mg / L, total nitrogen 8mg / L, ammonia nitrogen 4mg / L; impact resistance has been verified.
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