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Method for measuring murrayone content of iculatae medicinal material

A technology for the determination of Jiulixiang ketone and its method, which is applied in the field of content determination of drugs, can solve the problems of rare UPLC, etc., and achieve the effects of reducing solvent consumption, strong specificity, and accurate and reliable results

Active Publication Date: 2011-02-02
CHINA RESOURCES SANJIU MEDICAL & PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, UPLC has been used in the research of biopharmaceuticals, natural products, metabolomics, etc., but UPLC is rarely used in the analysis of traditional Chinese medicine.

Method used

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  • Method for measuring murrayone content of iculatae medicinal material
  • Method for measuring murrayone content of iculatae medicinal material
  • Method for measuring murrayone content of iculatae medicinal material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: Ultra-high performance liquid chromatography (UPLC) method is determined the content of jiuli ketone in the medicinal material of Thyme chinensis

[0046] a. Preparation of reference substance solution: Accurately weigh 5 mg of Jiulixiang ketone reference substance, add methanol to make a 0.05 mg / ml solution; b. Preparation of test solution: take 1.0 g of Thyme powder (passed through a No. 4 sieve), and accurately Weigh it, put it in a stoppered Erlenmeyer flask, add 25ml of methanol precisely, weigh it, use an ultrasonic processor with a power of 300W and a frequency of 40KHz for 30 minutes, let it cool, weigh it again, and make up the lost weight with methanol , shake well, filter, and take the filtrate to get it; c. Formulate chromatographic conditions: AcquityITY BEH C 18 The size is 2.1mm×50mm, 1.7μm chromatographic column, the mobile phase is 23:77 acetonitrile-water or 32:68 methanol-water, the flow rate is 0.5ml / min, the detection wavelength is 337...

Embodiment 2

[0047] Embodiment 2: Ultra-high performance liquid chromatography (UPLC) method is determined the content of jiuli ketone in the medicinal material of Thyme chinensis

[0048] a. Preparation of reference substance solution: accurately weigh 9 mg of jiulixiang ketone reference substance, and add methanol to make a 0.09 mg / ml solution; b. Preparation of test solution: take 2.0 g of thyme powder (passed through a No. 4 sieve), and accurately Weigh it, put it in a stoppered Erlenmeyer flask, add 50ml of methanol precisely, weigh it, use an ultrasonic processor with a power of 200W and a frequency of 80KHz for 50 minutes, let it cool, weigh it again, and make up the lost weight with methanol , shake well, filter, and take the filtrate to get it; c. Formulate chromatographic conditions: AcquityITY BEH C 18 The size is 2.1mm×50mm, 1.7μm chromatographic column, the mobile phase is 15:80 acetonitrile-water or 25:75 methanol-water, the flow rate is 0.8ml / min, the detection wavelength is...

Embodiment 3

[0049] Embodiment 3: Ultra-high performance liquid chromatography (UPLC) method is determined the content of jiuli ketone in the medicinal material of Thyme chinensis

[0050] a. Preparation of reference substance solution: Accurately weigh 3 mg of Jiulixiang ketone reference substance, add methanol to make a 0.03 mg / ml solution; b. Preparation of test solution: take 0.5 g of Thyme powder (passed through a No. 4 sieve), and accurately Weigh it, put it in a stoppered Erlenmeyer flask, add 15ml of methanol precisely, weigh it, use an ultrasonic processor with a power of 800W and a frequency of 50KHz for 15 minutes, let it cool, weigh it again, and make up the lost weight with methanol , shake well, filter, and take the filtrate to get it; c. Formulate chromatographic conditions: AcquityITY BEH C 18 The size of the chromatographic column is 2.1mm×50mm, 1.7μm, the mobile phase is 35:65 acetonitrile-water or 55:45 methanol-water, the flow rate is 0.3ml / min, the detection wavelength...

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Abstract

The invention discloses a method for measuring murrayone content of iculatae medicinal material. The murrayone content of the iculatae medicinal material is measured by ultra performance liquid chromatography (UPLC). The method has the advantages of strong specificity, good precision, high recovery rate, accurate and reliable result, simplicity and high speed. Compared with the HPLC method for measuring the murrayone content of the iculatae medicinal material, the UPLC method has the advantages that: the result is more accurate and reliable, the method is simpler and quicker, and greatly improves analysis speed and reduces the consumption of a solvent. The UPLC method can successfully substitute for the HPLC method and provides a new detection method for the quality control of the iculatae medicinal material.

Description

technical field [0001] The invention relates to a method for determining the content of medicines, in particular to a method for determining the content of jiurikone in the medicinal material Thyme thyme. Background technique [0002] Murraya panicula ta(L.)jack is a plant of Murraya paniculata (L.)jack in Rutaceae. It is pungent, slightly bitter, warm in nature and slightly poisonous. It has the functions of promoting qi and relieving pain, promoting blood circulation and dispelling blood stasis, expelling wind and dampness, anesthesia and relieving convulsions. For the treatment of stomachache, rheumatic arthralgia; external treatment of toothache, tumbling swelling pain, insect bites. Trichosanthes contains a variety of coumarins, flavonoids, alkaloids and terpenoids, of which kurikone is the main component. Pharmacological studies have shown that Thyme Thyme has anti-fertility and termination of pregnancy, anti-bacterial and anti-inflammatory effects, anti-thyroid, hyp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K135/00A61P1/04A61K36/75A61P17/02A61P29/00A61P1/02A61K127/00G01N30/36
Inventor 王静韩正洲刘志刚李琼娅谈英谭沛马舒冰
Owner CHINA RESOURCES SANJIU MEDICAL & PHARMA