Method for extracting tea oil by microwave-assisted aqueous enzymatic method
A microwave-assisted, water-enzymatic technology, which is applied in the production of fat/fat and fat production, can solve the problems of difficult extraction of tea saponin, high acid value of oil and low oil yield, etc., and achieves easy extraction and utilization and nutritional value. High, low-equipment effects
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Embodiment 1
[0018] The tea seeds are dehulled, dried and crushed. Weigh 4.00 g of crushed tea seeds, microwave for 60 s, add 12 ml of 0.05 mol / L sodium hydroxide salt solution, and bathe in 70°C water for 40 min. After cooling slightly, centrifuge and absorb the supernatant oil. Then use 18% hydrochloric acid solution to adjust the pH value to 4.5, add 0.5% compound enzyme (protease + cellulase), bathe in water at 40°C for 3 hours, and adjust the pH value to 8 with 10 mol / L sodium hydroxide solution ~9. Centrifuge and absorb the supernatant oil. Merge the camellia oil gained twice, vacuum dry, weigh. Finally, the yield of camellia oil was calculated to be 36.35%.
Embodiment 2
[0020] The tea seeds are dehulled, dried and crushed. Weigh 50.00 g of crushed tea seeds, microwave for 90 s, add 150 ml of 0.05 mol / L sodium hydroxide salt solution, and bathe in 80°C water for 30 min. After cooling slightly, centrifuge and absorb the supernatant oil. Then adjust the pH value of the degreased part to 4.0 with 20% hydrochloric acid solution, add 4% compound enzyme (protease + pectinase), bathe in water at 45°C for 2 hours, and adjust the pH value to 8 with 10 mol / L sodium hydroxide solution ~9. Centrifuge and absorb the supernatant oil. Merge the camellia oil gained twice, vacuum dry, weigh. Finally, the yield of camellia oil was calculated to be 37.82%.
Embodiment 3
[0022] The tea seeds are dehulled, dried and crushed. Weigh 100.00 g of crushed tea seeds, microwave for 80 s, add 300 ml of 0.05 mol / L sodium hydroxide salt solution, and bathe in 90°C water for 30 min. After cooling slightly, centrifuge and absorb the supernatant oil. Then adjust the pH to 4.5 with 20% hydrochloric acid solution, add 2% compound enzyme (protease + cellulase), bathe in water at 45°C for 4 hours, and adjust the pH to 8 with 10 mol / L sodium hydroxide solution ~9. Centrifuge and absorb the supernatant oil. Merge the camellia oil gained twice, vacuum dry, weigh. Finally, the yield of camellia oil was calculated to be 38.04%.
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