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Scale separation and purification method of pentaxanthin amylose

A technology for separation and purification of sea urchin yellow, applied in the field of biomedicine, can solve the problems of inconvenient large-scale industrial production of sea urchin yellow polysaccharide, reduce sample volume, and cannot remove impurities, etc., and achieve large-scale production with high value, good purity, and increased yield Effect

Inactive Publication Date: 2011-02-16
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The condition of this method is mild, but the disadvantage is that the efficiency is not high, and the yield of polysaccharide is low. Generally, it needs to be repeated 5 times or more to remove the protein.
This method requires the use of a large amount of organic reagents, strict operating requirements, and a long cycle
In addition, concentration under reduced pressure can only reduce the sample volume and cannot remove impurities, which cause inconvenience to the industrial scale production of echinacea polysaccharides

Method used

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  • Scale separation and purification method of pentaxanthin amylose
  • Scale separation and purification method of pentaxanthin amylose
  • Scale separation and purification method of pentaxanthin amylose

Examples

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Embodiment 1

[0029]Take 50kg of fresh Echinococcus glabra sea urchin as raw material, take the yellow and weigh about 10kg, cut it into small pieces, put it in a stirring tank, add an equal volume of acetone, stir it to make it fully contact with the acetone, let it stand for 30min, discard the upper layer of acetone , repeated 8 times until the acetone layer is basically colorless, finally the acetone is evaporated to dryness under reduced pressure, and ground to make 1.5kg of sea urchin yellow acetone powder. Put 1.5kg of sea urchin yellow acetone powder in 30 times of water (112L), cook at 90°C for 6h, extract twice, collect the extract, concentrate under reduced pressure to 56L. For papain enzymatic deproteinization, adjust the pH of sea urchin yellow extract to 6.5, add 0.6% (w / w) papain, incubate at 60°C for 24h, heat at 90°C for 15min to inactivate the enzyme, and collect the supernatant by centrifugation. Select an ultrafiltration membrane with a cut-off molecular weight of 100K to...

Embodiment 2

[0033] Take 100kg of fresh Echinococcus glabra sea urchin as the raw material, take the yellow and weigh about 20kg, cut it into small pieces, put it in a mixing tank, add an equal volume of acetone, stir it to make it fully contact with the acetone, let it stand for 30min, discard the upper layer of acetone , repeated several times until the acetone layer is substantially colorless (about 6-8 times), finally the acetone is evaporated to dryness under reduced pressure, and ground to make 3 kg of sea urchin yellow acetone powder. Put 3kg of sea urchin yellow acetone powder in 30 times of water (225L), cook at 90°C for 6h, extract twice, collect the extract, concentrate under reduced pressure to 112L. For papain enzymatic deproteinization, adjust the pH of sea urchin yellow extract to 6.5, add 0.6% (w / w) papain, incubate at 60°C for 20h, heat at 90°C for 15min to inactivate the enzyme, and collect the supernatant by centrifugation. Select an ultrafiltration membrane with a cut-o...

Embodiment 3

[0037] Take 200kg of fresh Echinococcus glabra sea urchin as the raw material, take the yellow and weigh about 40kg, cut it into small pieces, put it in a mixing tank, add an equal volume of acetone, stir it to make it fully contact with the acetone, let it stand for 30min, discard the upper layer of acetone , repeated several times until the acetone layer is basically colorless (about 6-8 times), finally the acetone is evaporated to dryness under reduced pressure, and ground to make 6 kg of sea urchin yellow acetone powder. Put 3kg of sea urchin yellow acetone powder in 30 times of water (450L), cook at 90°C for 6h, extract twice, collect the extract, concentrate under reduced pressure to 224L. For papain enzymatic deproteinization, adjust the pH of sea urchin yellow extract to 6.5, add 0.6% (W / W) papain, incubate at 60°C for 22h, heat at 90°C for 15min to inactivate the enzyme, and collect the supernatant by centrifugation. Select an ultrafiltration membrane with a cut-off m...

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Abstract

The invention relates to the field of biological medicines, in particular to a scale separation and purification method of pentaxanthin amylase. On the basis of the traditional extraction and separation method, The concentrating process of the invention comprises the flowing steps of: ultrafiltration concentration; chromatography by using a Cellulose DE52 ion exchange column; and then carrying out ultrafiltration concentration again; and chromatography by using a Sephacryl S-400HR gel filtration column; and finally removing impurities by dialysis. The invention puts emphasis on the separation and purification method of the pentaxanthin amylase and a scale purification technology thereof, not only can obtain high-yield and high-purity pentaxanthin amylase, but also provides a technological base for industrialized production.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a large-scale separation and purification method of echinacea yellow polysaccharide. Background technique [0002] Scientists from various countries have conducted extensive research on polysaccharides from different sources and found that polysaccharides have anti-tumor, cardiovascular disease treatment, and immune regulation effects. Echinacea yellow polysaccharide is a polysaccharide isolated and purified from Echinococcus photicus sea urchin. Its structure was identified by physicochemical analysis and GC analysis, and it was proved to be a novel structure glucan isolated from echinacea yellow for the first time. Pharmacological experimental studies have shown that sea urchin yellow polysaccharide not only has immunomodulatory activity, but also has natural antitumor activity. In 1985, lentinan was first developed into an injection in Japan and entered clinical application. As an ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12S3/02
Inventor 周长林奚涛高宇王慧窦洁
Owner CHINA PHARM UNIV
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