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Method for producing manninotriose and melibiose by fermenting soy molasses

A technology of mannotriose and soybean molasses, which is applied in the field of producing mannotriose and melibiose, can solve the problems of being unsuitable for mass production, complex operation, high cost, etc., and is suitable for industrial production, with simple processing methods and good quality Effect

Inactive Publication Date: 2011-02-16
天津实发中科百奥工业生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of them use boiling water to extract mannotriose from Rehmannia glutinosa, and there are also literature records to extract mannotriose by hydrolysis of threose, which can be divided into acid hydrolysis and enzymatic hydrolysis, but both are expensive and complicated to operate, and are not suitable for mass production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Preparation of spore suspension: Inoculate alcohol yeast (distillery yeast) TJZKBA10598 on the slant, incubate at 30°C for 3 days, add 0.75% normal saline to wash the mycelium, and obtain spore suspension. The spore content is about 10 5 -10 6 1, ready for use; the composition of the slant medium includes (1L): 20g sucrose, 5g yeast powder, KH 2 PO 4 0.5g, FeSO 4 · 7H 2 O 0.01g, urea 3g, agar 15g, dilute to 1L with distilled water, pH5.5.

[0019] (2) The above-mentioned spore suspension is used to inoculate into the sterilized liquid seed medium. The composition of the liquid seed medium includes (1L): 20g of sucrose, 5g of yeast powder, KH 2 PO 4 0.5g, FeSO 4 · 7H 2 O 0.01g, urea 3g, dilute to 1L with distilled water, pH value 5.5. The liquid seed culture was sterilized at 121°C for 15 minutes and then cooled; then the spore suspension was added to the above-mentioned cooled liquid seed medium according to the inoculum volume of 5% by volume (the liqu...

Embodiment 2

[0024] (1) Preparation of spore suspension: Inoculate alcohol yeast (distillery yeast) TJZKBA10598 on the slant, incubate at 30°C for 3 days, add 0.75% normal saline to wash the mycelium, and obtain spore suspension. The spore content is about 10 5 -10 6 1, ready for use; slant medium composition includes (1L): sucrose 20g, yeast powder 5g, KH 2 PO 4 0.5g, FeSO 4 · 7H 2 O 0.01g, urea 3g, agar 15g, dilute to 1L with distilled water, pH5.5.

[0025] (2) The above-mentioned spore suspension is used to inoculate into the sterilized liquid seed medium. The composition of the liquid seed medium includes (1L): 20g of sucrose, 5g of yeast powder, KH 2 PO 4 0.5g, FeSO 4 ·7H 2 O 0.01g, urea 3g, dilute to 1L with distilled water, pH value 5.5. The liquid seed culture was sterilized at 121°C for 15 minutes and then cooled; then the spore suspension was added to the above-mentioned cooled liquid seed medium according to the inoculum volume of 5% by volume (the liquid volume of...

Embodiment 3

[0030] (1) Preparation of spore suspension: Inoculate alcohol yeast (distillery yeast) TJZKBA10598 on the slant, incubate at 30°C for 3 days, add 0.75% normal saline to wash the mycelium, and obtain spore suspension. The spore content is about 10 5 -10 6 1, ready for use; slant medium composition includes (1L): sucrose 20g, yeast powder 5g, KH 2 PO 4 0.5g, FeSO 4 · 7H 2 O 0.01g, urea 3g, agar 15g, dilute to 1L with distilled water, pH5.5.

[0031] (2) The above-mentioned spore suspension is used to inoculate into the sterilized liquid seed medium. The composition of the liquid seed medium includes (1L): 20g of sucrose, 5g of yeast powder, KH 2 PO 4 0.5g, FeSO 4 ·7H 2 O 0.01g, urea 3g, dilute to 1L with distilled water, pH value 5.5. The liquid seed culture was sterilized at 121°C for 15 minutes and then cooled; then the spore suspension was added to the above-mentioned cooled liquid seed medium according to the inoculum volume of 5% by volume (the liquid volume of...

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Abstract

The invention relates to a method for producing manninotriose and melibiose by fermenting soy molasses, which comprises the following steps: (1) preparing a spore fungus suspension; (2) inoculating the spore fungus suspension into a sterilized liquid seed culture medium; (3) inoculating the primary liquid seed into a sterilized 5L seeding tank, and fermenting and culturing to obtain secondary liquid seed; (4) inoculating the secondary liquid seed into a sterilized fermentation tank, and fermenting and culturing by using soy molasses dilution as the culture medium; and (5) centrifugating the fermentation liquid to remove thalli, and carrying out chromatographic separation by using active carbon columns, thereby further obtaining manninotriose and melibiose crystals. The method is used for producing the manninotriose and the melibiose by fermenting the cheap material soy molasses, thereby effectively solving the problem of utilization of waste molasses; and the invention has the advantages of simple technique, high yield, good quality and low cost, does not need complicated equipment, and is beneficial to development and application of the manninotriose and the melibiose.

Description

technical field [0001] The invention relates to a method for producing mannotriose and melibiose, in particular to a method for fermenting soybean molasses to produce mannotriose and melibiose. Background technique [0002] Soybean molasses is a by-product obtained during the production of concentrated soybean protein. Soybean molasses is rich in soybean oligosaccharides, which can be used as raw materials for the production of functional soybean oligosaccharides. Wherein the main component content is: total sugar 54.64% (among them sucrose 32.17%, raffinose 4.27%, stachyose 17.54%), total lipid 18.00%, total steroid glycoside 3.06%, total phospholipid 8.79%, crude protein 8.71%, Ash content 8.69%. [0003] Melibiose is a disaccharide composed of D-galactose and D-glucose 1,6-combined. The chemical system name is O-α-galactopyranosyl-(1→6)-D-glucopyranose. It is an oligosaccharide that exists in nature and is contained in many plants, but its content is very small, and it...

Claims

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Application Information

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IPC IPC(8): C12P19/00C12P19/12C12R1/645
Inventor 徐勇虎李睿颖国华王永乐刘云胡娅君刘晓鸥
Owner 天津实发中科百奥工业生物技术有限公司
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