Lactobacillus plant arum ZY-40 and application thereof to preparing fish sausage through fermentation
A technology of Lactobacillus plantarum, ZY-40, applied in application, food preparation, microorganism-based methods, etc., to achieve good fermentation flavor and gel structure, good sliceability and chewiness
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Embodiment 1
[0025] Example 1: Screening, identification and preservation of strains
[0026] (1) MRS liquid medium: peptone 10g, beef extract 10g, yeast extract 5g, triamine citrate 2g, glucose 20g, Tween-80 1ml, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.5g, sulfuric acid Manganese 0.25g, distilled water 1000ml, pH value 6.2~6.4.
[0027] (2) Isolation of strains: Under aseptic conditions, the vinasse fish meat was added to sterilized physiological saline, homogenized under aseptic conditions, and after gradient dilution, the plate was poured by pouring and cultured at 30°C for 48 hours. Pick a single colony and continue streaking the plate until it is purified. The gram-positive and negative catalase test strains are suspected lactic acid bacteria.
[0028] (3) Re-screening of strains: the suspected lactic acid bacteria are subjected to glucose fermentation, gas production, viscous substance formation, hydrogen peroxide production, hydrogen sulfide production, ...
Embodiment 2
[0034] Example 2: Application of Lactobacillus plantarum ZY-40 in preparing fish meat fermented sausage
[0035] The processing technology mainly includes: surimi preparation → adding auxiliary materials, chopping → inoculation → filling → fermentation maturity → packaging
[0036] The specific operations are as follows:
[0037] (1) Surimi preparation: thawing frozen octopus and then dissecting it, removing scales, skins, heads, tails and internal organs, washing, and mincing meat.
[0038] (2) Chopping: Add 3% starch, 1.5% sucrose, 2% glucose and 2% salt at low temperature (5-10°C), and chopping for 20 minutes.
[0039] (3) Liquid culture of Lactobacillus plantarum ZY-40: After activation, Lactobacillus plantarum ZY-40 is inoculated into MRS liquid medium, and cultivated to the late logarithmic growth period at a temperature of 20-40°C and a rotation speed of 150 rpm. Adjust the concentration of bacteria.
[0040] (4) Inoculation: Add Lactobacillus plantarum ZY-40 to the chopped surim...
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