Ultrafiltration membrane-solid phase extraction combined device for sample pretreatment and application thereof

Abstract

The invention relates to an ultrafiltration membrane-solid phase extraction combined device for sample pretreatment and application thereof. Firstly, an ultrafiltration membrane for intercepting molecules greater than the specified molecular weight is selected and is cut into membrane sheets with the size same as the inner diameter of a small solid phase extraction column, and the ultrafiltration membrane is fixed at the upper layer of solid phase extraction materials through a sieve plate. Samples are extracted with organic solves, and the concentrated sample solutions are extracted, purified and enriched by the combined extraction device. The ultrafiltration membrane can simply, conveniently and effectively intercept and separate large molecules such as lipoid, protein, saccharide and the like according to the size of the membrane hole diameter, and can realize the synchronous separation, extraction, purification and enrichment of the trace target compounds in the complicated matrix samples through being in combined use with the solid phase extraction. The invention has low preparation cost, can easily realize mass production and automatic operation, can be applicable to complicated sample analysis capable of realizing various kinds of instrument analysis through multi-step pre-treatment, such as the sample analysis of biological tissue, metabolin and food, and can also be applied to the separation, purification or preparation of natural products and biomolecules.

Description

technical field [0001] The invention belongs to the field of chemical analysis instrument testing, and in particular relates to an efficient and practical ultrafiltration membrane-solid phase extraction combined device, which is suitable for the extraction, purification, Separation and enrichment. Background technique [0002] The detection of trace target compounds in samples with complex matrices often requires multi-step sample pretreatment before entering the instrument for analysis. Traditional sample pretreatment methods mainly include liquid-liquid extraction, Soxhlet extraction, ultrasonic-assisted extraction, etc., which have limitations to varying degrees, such as complicated operation process, high reagent consumption, and low separation efficiency. [0003] Solid-phase extraction technology is a sample pretreatment technology developed since the 1970s. It uses a solid-phase adsorbent to adsorb the target compound to separate it from the sample matrix and interfe...

AI Technical Summary

Problems solved by technology

However, most of the currently used commercial solid-phase extraction columns are filled with 30-80 μm granular materials, which have high requirements for the sample extraction solution, especially when there are interfering components such as biological macromolecules, proteins or lipids in the sample solution, it is easy to Cause blockage or contamination in the column, resulting in low separation column efficiency, poor adsorption efficiency and selectivity, which is limited in the application of trace target compound analysis in complex samples

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