Ultrafiltration membrane-solid phase extraction combined device for sample pretreatment and application thereof

A solid-phase extraction material and ultrafiltration membrane technology are applied in the field of ultrafiltration membrane-solid-phase extraction combined devices, which can solve the problems of low separation column efficiency, poor adsorption efficiency and selectivity, limitations and the like, and achieve simple operation and low cost. Effect

Inactive Publication Date: 2011-03-30
FOOD INSPECTION CENT OF CIQ SHENZHEN
View PDF0 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the currently used commercial solid-phase extraction columns are filled with 30-80 μm granular materials, which have high requirements for the sample extraction solution, especially when there are interfering components such as biological macromolecules, protei

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ultrafiltration membrane-solid phase extraction combined device for sample pretreatment and application thereof
  • Ultrafiltration membrane-solid phase extraction combined device for sample pretreatment and application thereof
  • Ultrafiltration membrane-solid phase extraction combined device for sample pretreatment and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0014] Example (Determination of various veterinary drug residues in spiked pig liver samples by LC-MS / MS using an ultrafiltration membrane-solid phase extraction device). The experimental steps are as follows:

[0015] 1. Weigh 5 g of pig liver (homogeneous), put it into a 50-mL polypropylene centrifuge tube with a stopper, add 50 μL of a mixed standard solution with a concentration of 100 μg / L prepared in methanol (addition concentration is 1 μg / kg), add 30 μL Glucuronidase and 8mL sodium acetate buffer solution (pH=5.5), fully mixed with a high-speed vortex for 1min, and shaken in a water bath at 37°C for 12h.

[0016] 2. Add 5g anhydrous Na 2 SO 4 (accurate to 0.01g), add 15mL of acetonitrile and vortex for 1min, reciprocate at room temperature for 20min, centrifuge at -4°C at 9500r / min for 5min, and carefully remove the upper organic layer to a 50-mL centrifuge tube. Add 15 mL of acetonitrile and vortex for 1 min, homogenize with a mechanical homogenizer at 13000 r / min...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an ultrafiltration membrane-solid phase extraction combined device for sample pretreatment and application thereof. Firstly, an ultrafiltration membrane for intercepting molecules greater than the specified molecular weight is selected and is cut into membrane sheets with the size same as the inner diameter of a small solid phase extraction column, and the ultrafiltration membrane is fixed at the upper layer of solid phase extraction materials through a sieve plate. Samples are extracted with organic solves, and the concentrated sample solutions are extracted, purified and enriched by the combined extraction device. The ultrafiltration membrane can simply, conveniently and effectively intercept and separate large molecules such as lipoid, protein, saccharide and the like according to the size of the membrane hole diameter, and can realize the synchronous separation, extraction, purification and enrichment of the trace target compounds in the complicated matrix samples through being in combined use with the solid phase extraction. The invention has low preparation cost, can easily realize mass production and automatic operation, can be applicable to complicated sample analysis capable of realizing various kinds of instrument analysis through multi-step pre-treatment, such as the sample analysis of biological tissue, metabolin and food, and can also be applied to the separation, purification or preparation of natural products and biomolecules.

Description

technical field [0001] The invention belongs to the field of chemical analysis instrument testing, and in particular relates to an efficient and practical ultrafiltration membrane-solid phase extraction combined device, which is suitable for the extraction, purification, Separation and enrichment. Background technique [0002] The detection of trace target compounds in samples with complex matrices often requires multi-step sample pretreatment before entering the instrument for analysis. Traditional sample pretreatment methods mainly include liquid-liquid extraction, Soxhlet extraction, ultrasonic-assisted extraction, etc., which have limitations to varying degrees, such as complicated operation process, high reagent consumption, and low separation efficiency. [0003] Solid-phase extraction technology is a sample pretreatment technology developed since the 1970s. It uses a solid-phase adsorbent to adsorb the target compound to separate it from the sample matrix and interfe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N1/34B01D15/08B01D61/18
Inventor 张毅岳振峰蓝芳沈金灿赵凤娟欧阳珊吴永宁
Owner FOOD INSPECTION CENT OF CIQ SHENZHEN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap