Preparation method of liquid inoculant of aboriginal probiotic bacillus megaterium, bacillus mucilaginosus and azotobacter chroococcum

A technology for Bacillus megaterium and jelly-like spores, which is applied in the field of preparation of liquid inoculants, can solve problems such as inability to save energy, complex production processes, application limitations, etc., and achieves reduction of processes and production processes for raw material preparation and autoclaving Simple, labor-saving effect

Inactive Publication Date: 2011-04-20
兰州市南北两山环境绿化工程指挥部 +2
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  • Claims
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AI Technical Summary

Problems solved by technology

[0002] The preparation method of the traditional microbial liquid bacterial agent uses glucose, peptone, molasses, beef extract, vitamins, inorganic salts and water to prepare a liquid medium, and scrapes the bacterial lawn from the slant of the test tube to inoculate it in the liquid medium , prepared by aerobic or anaerobic culture into a liquid bacterial agent, its disadvantages are: the cost of raw materials used is high, the production process is complicated, and energy cannot be saved
Its disadvantages are: complex preparation process, long fermentation cycle, high cost and limited application

Method used

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  • Preparation method of liquid inoculant of aboriginal probiotic bacillus megaterium, bacillus mucilaginosus and azotobacter chroococcum
  • Preparation method of liquid inoculant of aboriginal probiotic bacillus megaterium, bacillus mucilaginosus and azotobacter chroococcum
  • Preparation method of liquid inoculant of aboriginal probiotic bacillus megaterium, bacillus mucilaginosus and azotobacter chroococcum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: a kind of indigenous probiotic bacillus megaterium, jelly-like bacillus and torus brown nitrogen-fixing bacteria liquid inoculum, by bacillus megaterium (Bacillus megaterium), jelly-like bacillus (Bacillus mucilaginosus), jelly-like azotobacter ( Azotobacter chroococcum) and liquid culture medium, the percentage by weight of its three bacterial strains is 55-65% for Bacillus megaterium, 30-35% for Bacillus jelly-like, and 5-10% for Azotobacter rotundum. The preservation number of the bacillus is CGMCC No.3770; the preservation number of the jelly-like bacillus is CGMCC No.3769;

[0027] The liquid medium includes adding 1-2 g of ammonium sulfate and 0.5-1 ml of phosphoric acid to 1 liter of fresh tofu wastewater or vermicelli wastewater or corn starch wastewater or potato starch wastewater, and adjusting the pH to 7.2-7.5 with 1% lime water.

Embodiment 2

[0028] Example 2: see figure 1 , a preparation method of indigenous probiotic Bacillus megaterium, Bacillus jelly-like and Azotobacter torusus liquid inoculum, which has the following steps:

[0029]A. Preparation of indigenous probiotic Bacillus megaterium and Bacillus jelly-like agar slant medium and cultivation of strains:

[0030] a. Preparation of agar slant medium: 0.5-2% wort juice, 0.5-2% glucose, 0.2-0.6% peptone, 1-3% agar powder, 80-120ml sterile water, after heating and dissolving, use 1% Adjust the pH value to 7.0-7.5 with sodium hydroxide, steam sterilize at 110-114°C for 30-35 minutes, and then subpackage under aseptic conditions into dry and sterilized test tubes of 15×1.5cm, set them on an inclined plane and wait for solidification. use;

[0031] b. Cultivation of indigenous probiotic Bacillus megaterium and Bacillus jelly-like strains: respectively take the mother species of Bacillus megaterium and Bacillus jelly-like on the agar slant, and transfer them t...

Embodiment 3

[0043] Embodiment 3: see figure 1 , a preparation method of indigenous probiotic Bacillus megaterium, Bacillus jelly-like and Azotobacter torusus liquid bacterial preparation, it has the following steps: Steps A-B are the same as in Example 2,

[0044] C Indigenous probiotics Bacillus megaterium, Bacillus jelly-like, Azotobacter rotundum liquid culture medium preparation and liquid bacteria:

[0045] (1) For the cultivation of test tube liquid strains, get 100ml of fresh vermicelli waste water, add (NH 4 ) 2 SO 4 0.1g, H 3 PO 4 0.05ml, after fully dissolving, adjust the pH to 7.0~7.5, divide into dry test tubes of 15×1.2cm, autoclave at 112°C for 30 minutes, cool to 32°C, inoculate Bacillus megaterium and jelly-like spores respectively Bacillus and Azotobacter rotundum are cultured in test tube liquid culture medium for 24 to 48 hours at 28 to 32°C to become test tube liquid strains (secondary strains).

[0046] (2) The cultivation of Erlenmeyer flask liquid strain:

...

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Abstract

The invention relates to a preparation method of a microbial inoculant, in particular to a preparation method of a liquid inoculant combining bacillus megaterium, bacillus mucilaginosus and azotobacter chroococcum by utilizing waste liquor generated in the food industry. The liquid inoculant of the aboriginal probiotic bacillus megaterium, the azotobacter chroococcum and the bacillus mucilaginosus is mainly characterized in being prepared from 55-65 percent by weight of bacillus megaterium, 30-35 percent by weight of bacillus mucilaginosus, 5-10 percent by weight of azotobacter chroococcum and a fluid nutrient medium, wherein the bacillus megaterium has the preservation number of CGMCC (China General Microorganism Conservation Center) NO.3770, the bacillus mucilaginosus has the preservation number of CGMCC No.3769, and the azotobacter chroococcum has the preservation number of CGMCC No.3768. The invention has the advantages that the waste liquor generated in the food industry is used as a raw material, three-waste pollution is not generated in the production process, the production process is simple and convenient to operate and saves labor and energy, and the liquid inoculant can be used as an organic wastewater purifier or a microbial fertilizer and a microbial feed.

Description

Technical field: [0001] The invention mainly relates to a method for preparing a microbial inoculum, in particular to a method for preparing a liquid inoculum of a combination of Bacillus megaterium, Bacillus jelly-like and Azotobacter rotundum by using waste liquid produced in the food industry. Background technique: [0002] The preparation method of the traditional microbial liquid bacterial agent uses glucose, peptone, molasses, beef extract, vitamins, inorganic salts and water to prepare a liquid medium, and scrapes the bacterial lawn from the slant of the test tube to inoculate it in the liquid medium , prepared by aerobic or anaerobic culture into liquid bacterial agents, its disadvantages are: the cost of raw materials used is high, the production process is complicated, and energy cannot be saved. [0003] The traditional method of mixing bacterial agents is to cultivate various bacterial species (aerobic and anaerobic) in different growth environments, and then scr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20C02F3/34C05F11/08A23K1/16C12R1/11C12R1/07C12R1/065A23K10/18
Inventor 孙荣高钟芳李春杰赵瑾柴青
Owner 兰州市南北两山环境绿化工程指挥部
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