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Hydroxy-terminated hyperbranched polyamine-ester polymer and application thereof in microfluidic chip

A hydroxyl-terminated hyperbranched, microfluidic chip technology, applied in the field of polymers, can solve problems such as detection and separation of unfavorable separation substances, reduced detection efficiency of detectors, and increased sample diffusion, and achieves improved hydrophilicity, inhibition of adsorption, The effect of easy availability of raw materials

Inactive Publication Date: 2011-05-18
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current method of connecting the microfluidic chip and the capillary is easy to cause dead volume at the connection. If there is a dead volume, there will be hysteresis, increased sample diffusion, peak broadening, tailing, etc., resulting in a decrease in the detection efficiency of the detector. , which is not conducive to the detection and separation of separated substances

Method used

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  • Hydroxy-terminated hyperbranched polyamine-ester polymer and application thereof in microfluidic chip
  • Hydroxy-terminated hyperbranched polyamine-ester polymer and application thereof in microfluidic chip
  • Hydroxy-terminated hyperbranched polyamine-ester polymer and application thereof in microfluidic chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Preparation of G2 generation hydroxyl-terminated hyperbranched polyamine-ester polymer

[0042] Pass nitrogen gas into a 250mL three-necked flask for 10 minutes, take 0.2mol diethanolamine, 0.2mol methyl acrylate and 10ml methanol, mix them evenly in a small beaker and add them to the three-necked flask, and ventilate the mixture with nitrogen gas at room temperature for 10 minutes, stir for 30 minutes and heat up to 35°C After keeping for 4 hours, the methanol was distilled off under reduced pressure to obtain a colorless and transparent oily substance N, N-dihydroxyethyl-3-aminopropionic acid methyl ester, the reaction formula is shown below.

[0043]

[0044] Add 0.045mol of synthesized monomers, 0.045g of p-toluenesulfonic acid and 0.005mol of trimethylolpropane into a 250mL four-neck flask, pass nitrogen gas for 10min, stir until dissolved, then raise the temperature to 85°C and keep it constant, stir for 24h, and then The methanol and unreacted small ...

Embodiment 2

[0046] Example 2 Preparation of G3 generation hydroxyl-terminated hyperbranched polyamine-ester polymer

[0047] Add 0.1mol of N,N-dihydroxyethyl-3-aminopropionic acid methyl ester synthesized in Example 1, 0.0986g of p-toluenesulfonic acid and 0.004761mol of trimethylolpropane into a 250mL four-necked flask, and pass nitrogen gas for 10min , stirred to be dissolved, then heated to 85°C and kept constant, stirred for 24 hours, and then distilled off the methanol and unreacted small molecular compounds in the reaction to obtain the G3 generation of hydroxyl-terminated hyperbranched polyamine-ester polymer. Wherein, the molar ratio of trimethylolpropane and N, N-dihydroxyethyl-3-aminopropionic acid methyl ester is 1: 21, and the structural formula of the G3 generation terminal hydroxyl hyperbranched polyamine-ester polymer is as mentioned above Show.

Embodiment 3

[0048] Example 3 Preparation of G4 generation hydroxyl-terminated hyperbranched polyamine-ester polymer

[0049] Add 0.2mol of N,N-dihydroxyethyl-3-aminopropionic acid methyl ester synthesized in Example 1, 0.194g of p-toluenesulfonic acid and 0.004448mol of trimethylolpropane into a 250mL four-necked flask, and pass nitrogen gas for 10min , stirred to be dissolved, then heated to 85°C and kept constant, stirred for 24 hours, and then distilled to remove methanol and unreacted small molecular compounds in the reaction to obtain the G4 generation of hydroxyl-terminated hyperbranched polyamine-ester polymer. Wherein, the molar ratio of trimethylolpropane and N, N-dihydroxyethyl-3-aminopropionic acid methyl ester is 1: 45, and the structural formula of the G4 terminal hydroxyl hyperbranched polyamine-ester polymer is as mentioned above Show.

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Abstract

The invention relates to hydroxy-terminated hyperbranched polyamine-ester polymer and an application thereof in a microfluidic chip. The preparation method of the hydroxy-terminated hyperbranched polyamine-ester polymer comprises the following steps: using equimolar diethanol amine and methyl acrylate to react and obtain N,N-dihydroxyethyl-3-aminomethyl propionate; and using trimethylolpropane and N,N-dihydroxyethyl-3-aminomethyl propionate to react, wherein G2 hydroxy-terminated hyperbranched polyamine-ester polymer, G3 hydroxy-terminated hyperbranched polyamine-ester polymer and G4 hydroxy-terminated hyperbranched polyamine-ester polymer are obtained respectively when the molar ratio of trimethylolpropane to N,N-dihydroxyethyl-3-aminomethyl propionate is 1:9, 1:21 or 1:45. The hydroxy-terminated hyperbranched polyamine-ester polymer is used to prepare the hydrophilic microfluidic chip. The preparation method of the chip comprises the following steps: oxidizing a polydimethylsiloxane (PDMS) microfluidic chip substrate in oxygen atmosphere; and connecting silane coupling agent with the inside of the substrate, and connecting the polymer with silane coupling agent. By strictly controlling the reaction conditions and the molar ratio of reactants, relatively regular polymer can be prepared. The polymer in the invention is used to modify PDMS, thus the hydrophilicity can be maintained permanent, any expensive instrument is not used and the preparation is convenient and fast.

Description

technical field [0001] The invention relates to the field of polymers, in particular to a hydroxyl-terminated hyperbranched polyamine-ester polymer, and also relates to the application of the polymer in microfluidic chips. Background technique [0002] Since Manz et al. proposed the microfluidic analysis system in the 1990s, microfluidic chip technology has developed rapidly and has been applied in many aspects. Microfluidic chip technology has the advantages of fast separation speed, small sample consumption, and fast detection speed. Polydimethylsiloxane has become one of the main materials for making microfluidic chips because of its cheap price, stable chemical properties, durability, good light transmission, and excellent biocompatibility. However, PDMS itself has some defects. , such as the highly hydrophobic surface, which will cause air bubbles in the separation channel during the separation process, resulting in disconnection of the flow path, resulting in test fai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G63/685G01N35/00
Inventor 寿崇琦杨文林栋徐磊刘冰蒋大庆
Owner UNIV OF JINAN
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