Method for identifying pathogenicity of sesame stem blight and blast pathogenic bacteria

Abstract

The invention relates to a method for identifying pathogenicity of sesame stem blight and blast pathogenic bacteria. The method comprises the following steps of: (1) culturing bacterial strains for inoculation; (2) pretreating seeds, namely selecting healthy and full sesame seeds and sterilizing for later use; (3) inoculating, namely adding a sterilization culture medium into a culture bottle, laying down sterile filter paper, adding sterile water, removing bubbles, uniformly placing sesame sterile seeds along the edges of the filter paper, inoculating bacterium cakes in the center of the filter paper, and hermetically culturing in the dark; (4) investigating pathogenicity, namely dividing the pathogenic conditions into different pathogenic grades according to pathogenic degrees; and (5) evaluating the pathogenicity, wherein the pathogenic index is equal to Sigma (pathogenic grade*corresponding pathogenic bud number) / total bud number. The method is simple and convenient and practicable, the required equipment is few, the result is reliable, and the pathogenicity of different sesame stem blight pathogenic bacteria and blast pathogenic bacteria can be reflected more accurately.

Description

technical field [0001] The invention relates to a method for identifying the pathogenicity of sesame stem point blight and fusarium wilt pathogenic bacteria. Background technique [0002] Sesame is one of the four important oil crops in my country, and its planting is widely distributed. Among them, the sesame planting area in the Huanghuai and the middle and lower reaches of the Yangtze River is relatively concentrated, accounting for 70% of the national sesame area. The growth period of sesame is about 90 days. It is a temperature-loving crop and generally grows from June to August. At this time, most of the main sesame producing areas are in high temperature and rainy seasons, and high temperature and high humidity can easily induce sesame stem point blight and sesame wilt . [0003] Sesame stem point blight is also known as stem rot and charcoal rot, and its pathogenic bacteria is Macrophomina phaseoli (Maubl.) Ashby., which belongs to the fungus of the subphylum Deuter...

AI Technical Summary

Problems solved by technology

The pathogenicity identification method of sesame stem point blight and sesame wilt generally adopts the combination of natural disease in the field and artificial inoculation (spraying spores or sclerotia suspensions at the flowering stage, or inoculating diseased wheat kernels in the soil), and investigating the pathogenicity of different sesame materials. Disease index, but this method of field adult plant stage identification requires large-scale field moisturizing during the inoculation period, and it is difficult to control the conditions in large areas of the field, and is also easily disturbed by external environmental factors such as climate, and these two pathogens can be removed from the soil. In addition, it also contains a variety of pathogenic bacteria of soil-borne diseases, so this method of field inoculation identification at the adult plant stage is not only cumbersome but also has low reliability and a long cycle, and it is impossible to simultaneously identify multiple pathogenic bacteria

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