Denitrified microbial agent as well as preparation method and application thereof

A microbial inoculant and denitrification technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of restricting large-scale popularization and use, low investment cost, high investment and operation cost, and achieve short processing time. , reducing the total nitrogen index, the effect of broad application prospects

Inactive Publication Date: 2011-05-25
WUXI ZHONGJIA TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the installation of denitrification devices in sewage treatment can reduce the total nitrogen index in water, the high investment and operation costs limit its large-scale promotion and use
The biological denitrification method only needs to add denitrification microbial agents in the traditional sewage treatment process, no

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0033] Example 1

[0034] Preparation of denitrification microbial agents:

[0035] Microbacterium oxydans MCCC 1A04 fermentation broth, Rhodococcus ruber ATCC 51882 fermentation broth, and denitrifying bacteria Paracoccus denitrificansATCC 17741 fermentation broth were prepared according to the ratio of 30%, 20%, and 50% respectively (the percentage is the percentage of bacteria count to the total bacteria count ) Mix and add the solid auxiliary material, the mass ratio of the solid auxiliary material to the fermentation broth is 1.5:1, stir evenly, dry at 28°C, pulverize, sieving through 40 mesh, packaging and bagging. Wherein, the fermentation broth of Microbacterium oxydans MCCC 1A04, the fermentation broth of Rhodococcusruber ATCC 51882 and the fermentation broth of the denitrifying bacteria Paracoccus denitrificans ATCC 17741 are all obtained by slant culture, primary seed culture and fermentation culture. The slant medium, first-level seed culture and fermentation culture i...

Example Embodiment

[0038] Example 2

[0039] Preparation of denitrification microbial agents:

[0040] Microbacterium oxydans CGMCC 1788 fermentation broth, Rhodococcus ruber ATCC 15906 fermentation broth, and denitrifying bacteria Paracoccus denitrificansATCC 13543 fermentation broth were mixed according to the ratio of 50%, 20%, and 30% respectively (the percentage is the percentage of bacteria count to the total bacteria count ) Mix and add the solid auxiliary material, the mass ratio of the solid auxiliary material to the fermentation broth is 2:1, stir evenly, dry at 40°C, pulverize, sieving through 40 mesh, packaging and bagging.

[0041] Among them, the fermentation broth of Microbacterium oxydans CGMCC 1788, the fermentation broth of Rhodococcus ruber ATCC 15906, and the fermentation broth of the denitrifying bacteria Paracoccus denitrificans ATCC 13543 are all obtained by slant culture, primary seed culture and fermentation culture. The slant medium, first-level seed culture and fermentation ...

Example Embodiment

[0044] Example 3

[0045] Preparation of denitrification microbial agents:

[0046] The fermentation broth of Microbacterium oxydans MCCC 1A04974, the fermentation broth of Rhodococcus ruber ATCC 534 and the fermentation broth of denitrifying bacteria Paracoccus denitrificansATCC 35512 were prepared according to the ratio of 40%, 20%, and 40% respectively (the percentage is the percentage of bacteria count to the total bacteria count. ) Mix and add the solid auxiliary material, the mass ratio of the solid auxiliary material to the fermentation broth is 1.8:1, stir evenly, dry at 35°C, crush, sieving through 40 mesh, packaging and bagging.

[0047] Among them, the fermentation broth of Microbacterium oxydans MCCC 1A04974, the fermentation broth of Rhodococcus ruber ATCC 534 and the fermentation broth of the denitrifying bacteria Paracoccus denitrificans ATCC 35512 are all obtained through slant culture, first-level seed culture and fermentation culture. The slant medium, first-level ...

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Abstract

The invention discloses a denitrified microbial agent as well as a preparation method and application thereof. The denitrified microbial agent contains Microbacterium oxydans, Rhodococcus and Paracoccus denitrificans. The preparation method comprises the following steps of: uniformly mixing a Microbacterium oxydans fermentation liquor, a Rhodococcus fermentation liquor and a Paracoccus denitrificans fermentation liquor with solid auxiliary materials according to a ratio; drying at 28-40 DEG C; pulverizing; and sieving with 10-50 meshes. The denitrified microbial agent is in a dormant state atnormal temperature in vacuum, can be used for quickly and effectively reducing the total nitrogen index in water in a sewage treatment process, and has wide application prospect.

Description

technical field [0001] The invention belongs to the field of microbial environmental protection, and in particular relates to a denitrification microbial bacterial agent and its preparation method and application. Background technique [0002] With the continuous development of modern industry, the widespread application of chemical fertilizers and the discharge of a large amount of domestic and industrial sewage, the nitrogen pollution in wastewater is becoming more and more serious. The nitrogen source in sewage is the nutrient of the water body, but the eutrophication of the water body can easily cause the water body to be black and smelly. Excessive algae and ammonia in the source water will increase the cost of water treatment. Combined nitrogen (such as nitrite and nitrate, etc.) has toxic side effects on humans and organisms, especially nitrite, which is a carcinogen, should be Strictly control emission standards. At present, the BOD, COD and TP of most sewage treat...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C02F3/34C12R1/01
Inventor 朱宝泉胡海峰陈纪文张琴陈建昌赵晓鸣
Owner WUXI ZHONGJIA TECH
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