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Method for detecting BHA (butylated hydroxyanisole) in edible oil and plastic packages by laser nanometer Raman spectroscopy

A spectroscopic detection and plastic packaging technology, applied in Raman scattering, material excitation analysis, etc., can solve the problems of long analysis time and high operation requirements, and achieve the effects of high sensitivity, low detection limit, and convenient and fast method.

Inactive Publication Date: 2011-06-15
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the detection methods for such antioxidants mainly include gas chromatography, capillary electrophoresis, high performance liquid chromatography, ultra-high performance liquid chromatography-mass spectrometry, etc., but the operation requirements are high and the analysis time is long

Method used

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  • Method for detecting BHA (butylated hydroxyanisole) in edible oil and plastic packages by laser nanometer Raman spectroscopy
  • Method for detecting BHA (butylated hydroxyanisole) in edible oil and plastic packages by laser nanometer Raman spectroscopy
  • Method for detecting BHA (butylated hydroxyanisole) in edible oil and plastic packages by laser nanometer Raman spectroscopy

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preparation example Construction

[0023] B. The preparation method of surface enhancer nano-gold solution is: accurately weigh 0.0300 g of chloroauric acid or sodium salt, and configure it with ultrapure water to 6 × 10 -4 g / ml solution, stir and boil, quickly add sodium citrate solution, continue to stir for 20-40min, cool to room temperature for later use; particle size requirement: about 30nm.

[0024] Of course, those skilled in the art can also formulate other laser Raman spectrometer parameters and surface enhancers according to known materials;

[0025] After determining the detection conditions, the standard curve can be drawn by referring to the following methods:

[0026] Accurately weigh 100mg of BHA, dissolve it with an appropriate amount of methanol, transfer it to a 10mL volumetric flask, and use methanol to make up the volume; shake it up and let it stand still to get a 10ug / L BHA standard solution, which is gradually diluted with the standard solution to make 1.0, 0.1 , 0.01ug / L series solutio...

Embodiment 1

[0029] Cut the PVC material into fragments of about 0.5cm×0.5cm, accurately weigh 0.5g from the fragments, add 10mL of chloroform and ultrasonically extract to dissolve all the samples, then add methanol to make the solution volume to 25mL, and wait for the solution to precipitate After filtering, the filtrate was evaporated to dryness at 40°C, and the volume was adjusted to 2 mL with methanol, and passed through a 0.45 μm filter membrane for Raman detection. Three parallel samples were made for the PVC material, namely parallel sample 1, parallel sample 2, and parallel sample 3. The analysis results are shown in Table 1. The parallel sample 1 was repeatedly measured 6 times, and the analysis results are shown in Table 2. Through analysis, the relative standard deviation (RSD) of the parallel samples is 4.97, and the relative standard deviation of the repeated measurement experiment is 2.92%, which shows good repeatability.

[0030] Table 1 BHA content in PVC materials

[00...

Embodiment 2

[0035] Accurately weigh 2ml of edible oil, add 20mL of chloroform for three extractions, combine the organic layer solution with methanol to make the solution volume to 25mL, rotary evaporate the solution at 40°C, and dilute to 2mL with methanol for Raman detection. Three parallel samples were made for the edible oil sample, namely parallel sample 1, parallel sample 2, and parallel sample 3. The analysis results are shown in Table 3. Parallel sample 1 was repeated 6 times, and the analysis results are shown in Table 4. Through analysis, the relative standard deviation (RSD) of the parallel samples is 0.648, and the relative standard deviation of the repeated measurement experiment is 0.892%, and the repeatability is good.

[0036] Table 3 BHA content in edible oil

[0037]

[0038] Table 4 Repeated determination results of parallel sample 1

[0039] Numbering

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Abstract

The invention relates to a method for detecting BHA (butylated hydroxyanisole) in edible oil and plastic packages by laser nanometer Raman spectroscopy. The method comprises the following steps: setting the parameters and scanning conditions of a laser Raman spectrometer, and preparing a nano-gold solution with a certain particle size; scanning a series of BHA standard solutions with a certain concentration gradient and plotting a standard curve; extracting BHA in the packaging material by an organic solvent, precipitating, filtering, concentrating, fixing volume, and the like; mixing BHA methanol solution with the prepared nano-gold colloidal solution at the ratio of 1 to 10; and adjusting pH value; and carrying out laser scanning. The method has the advantages of low detection limit, high detection speed, less consumption of analytical reagent, and low detection cost, and can achieve rapid quantitative and qualitative detection of BHA in the sample.

Description

Technical field: [0001] The invention relates to a method for detecting antioxidant BHA in food and food packaging materials, in particular to a method for detecting antioxidant BHA in plastic packaging materials and edible oils with laser nano-enhanced Raman spectroscopy. Background technique: [0002] Butylated hydroxyanisole (BHA) is a good antioxidant that can be used in food and food packaging materials. It is necessary to add an appropriate amount of antioxidants to maintain the original properties or nutritional value of food or packaging materials. As a fat-soluble antioxidant, BHA is suitable for oily food and fat-rich food, and can also be used as a feed antioxidant. However, excessive use of antioxidants has certain toxic and carcinogenic effects on the human body. GB9685-2008 stipulates that the maximum usage of BHA in food packaging materials is 0.1% and the specific migration limit is 30mg / kg. As a food additive, it is stipulated that the maximum usage amount...

Claims

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Application Information

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IPC IPC(8): G01N21/65
Inventor 姚卫蓉孙莹莹汪仕韬
Owner JIANGNAN UNIV
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