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Method for preparing polypeptide by taking seashells as raw material

A shell and raw material technology, applied in the field of peptide preparation, can solve the problems of no antibacterial peptide research, and achieve high potential market value, good economic and social benefits, and low prices

Inactive Publication Date: 2011-07-06
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0025] At present, antimicrobial peptides derived from shellfish are mainly extracted from the flesh or blood of shellfish, and there is almost no research on antimicrobial peptides from shellfish shells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1) Wash the shells with 10% dilute hydrochloric acid, dry and crush them to 2 mesh at normal temperature;

[0039] 2) Soak the crushed shell powder with 0.1% sodium hydroxide solution for 10 hours (to extract shell protein), the liquid-solid ratio is 100ml / g (L / Kg), and treat it with ultrasonic wave for 300 minutes;

[0040] 3) filtering the solid-liquid mixture to obtain a filtrate;

[0041] 4) The filtrate is extracted with acetone, separated and purified to obtain the polypeptide.

Embodiment 2

[0043] 1) Clean the shells with 0.1% dilute sulfuric acid, then dry and crush them to 10000 mesh at 200°C;

[0044] 2) Soak the crushed shell powder with 10% potassium hydroxide solution for 120 hours (to extract shell protein), the liquid-solid ratio is 2ml / g (L / Kg), and treat it with microwave for 300 minutes;

[0045] 3) filtering the solid-liquid mixture to obtain a filtrate;

[0046] 4) The filtrate is extracted with ethanol, separated and purified to obtain the polypeptide.

Embodiment 3

[0048] 1) Clean the shells with 2% dilute nitric acid, then dry and crush them to 100 mesh at 80°C;

[0049] 2) Soak the crushed shell powder with 2% potassium hydroxide solution for 24 hours (to extract shell protein), the liquid-solid ratio is 10ml / g (L / Kg), and treat it with ultrasonic wave for 60 minutes;

[0050] 3) filtering the solid-liquid mixture to obtain a filtrate;

[0051] 4) The filtrate is extracted with ethylene-propanol, separated and purified to obtain the polypeptide.

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Abstract

The invention discloses a method for preparing polypeptide by taking seashells as a raw material. The method comprises the following steps of: (1) cleaning the seashells with a cleaning solution and drying and grinding at normal temperature or at the temperature between 40 DEG C and 200 DEG C to obtain seashell powder; (2) soaking the seashell powder in an alkali solution for 10-120 hours and treating with ultrasonic waves or microwaves for 5-300 minutes to obtain a solid-liquid mixture with the liquid to solid ratio between 2 ml / g and 100 ml / g; (3) filtering the solid-liquid mixture to obtain a filtrate; and (4) extracting the filtrate with an organic solvent, separating and purifying to obtain the polypeptide. The method for preparing the polypeptide by taking the seashells as the raw material has the advantages of simple technology and mild operating condition. The prepared product has potential application in the development of products such as an antibacterial polypeptide medicament, an agricultural fungicide, a pesticide, a preservative, a surface glazing agent and the like.

Description

technical field [0001] The invention relates to the field of shell utilization, in particular to a method for preparing polypeptides from shells. Background technique [0002] Shellfish is the main species of mariculture in my country, accounting for more than 70% of the total output of mariculture in the country, with a total output of 12 million tons per year, accounting for more than 60% of the world's total output, ranking first in the world. [0003] The rapid development of shellfish farming has produced a large amount of shell waste. For a long time, a large number of shell resources have not been fully utilized, and they are dumped on the coast and beaches as waste, causing serious coastal pollution. Shell pollution has become an urgent environmental problem in coastal areas, and the environmental problems caused by the accumulation of shells are becoming more and more serious year by year. The problem of shell disposal has become one of the bottlenecks restricting...

Claims

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Application Information

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IPC IPC(8): C07K1/14
Inventor 夏枚生叶瑛李海晏张路
Owner ZHEJIANG UNIV
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