Method for culturing baby hamster kidney (BHK) 21 cell in serum-free way, and vaccine preparation method

Abstract

The invention provides a method for culturing baby hamster kidney (BHK) 21 cell in a serum-free way, which leads the BHK 21 cell to be inoculated into a cell culture medium for culturing, wherein the cell culture medium comprises a basic culture medium and further comprises 100-200g / 100L of soy protein, 100-200g / 100L of pea protein, 100-200g / 100L of broad bean protein, 0-100g / 100L of potato protein, 0-200g / 100L of wheat gluten protein and 50-100g / 100L of rice protein by taking the volume of solvent of the culture medium as reference. In addition, the invention also provides a vaccine (such asrabies vaccine and foot-and-mouth disease vaccine) preparation method comprising the method for culturing the BHK 21 cell. The BHK 21 cell cultured by the culture medium containing the vegetable protein is high in culture density, beneficial to separating down-stream products of the cells, low in cost, small in batch difference, good in safety and suitable for producing virus host, expression vector and the like of biological products such as vaccine and the like.

Description

technical field [0001] The invention relates to a method for cultivating BHK21 cells and a method for preparing a vaccine, in particular to a method for cultivating BHK21 cells without serum and a method for preparing a vaccine comprising the method for cultivating BHK21 cells. Background technique [0002] In the existing methods for culturing BHK21 cells without serum, soybean hydrolyzate is generally added as a component of serum-free animal cell culture medium to improve the stability and culture density of cultured BHK21 cells, increase the yield and utilization of cultured BHK21 cells Yield of recombinant target protein products produced by BHK21 cells as expression vectors. And it is believed that hydrolysates of other plants, such as wheat hydrolysates, do not obtain comparable beneficial effects as soybean hydrolysates. [0003] For example, CN 1318577C (Patent No. 00816020.1) discloses a method for cultivating mammalian cells, which uses a protein-free and serum-f...

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the shortcomings of low cell culture density, high cost of downstream products of isolated cells, large batch differences of cultured cells and poor safety in the existing method for culturing BHK21 cells without serum, and to provide a new serum-free method. A method for cultivating BHK21 cells, the method has high cell culture density, facilitates the separation of downstream products of cells, low cost, small batch differences of cultured cells, and good safety

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