Method for preparing panax japonicus saponin IVa and application of panax japonicus saponin IVa in preparing a medicament for protecting liver and lowering transaminase

A technology for ginseng saponins and pharmaceutical preparations, applied in the directions of drug combinations, pharmaceutical formulations, steroids, etc., can solve the problems of complex processes and low purity of ginseng saponins IVa monomers, and achieve high purity, stable content, Curative effect

Inactive Publication Date: 2011-07-27
SHAANXI UNIV OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The invention provides a preparation method of bamboo ginseng saponin IVa, to solve the problems of complex technology in the prior art and the low purity of the prepared bamboo ginseng saponin IVa monomer

Method used

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  • Method for preparing panax japonicus saponin IVa and application of panax japonicus saponin IVa in preparing a medicament for protecting liver and lowering transaminase
  • Method for preparing panax japonicus saponin IVa and application of panax japonicus saponin IVa in preparing a medicament for protecting liver and lowering transaminase
  • Method for preparing panax japonicus saponin IVa and application of panax japonicus saponin IVa in preparing a medicament for protecting liver and lowering transaminase

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preparation example Construction

[0029] One, the preparation method of bamboo ginseng saponin IVa

[0030] The processing steps and technical conditions of this preparation method are as follows:

[0031] (1) Extraction Crush the raw medicinal material of Ginseng ginseng, extract the broken material with 5-40 times of water or a hydrophilic organic solvent, extract 1-3 times, each time for 1-3 hours, filter, and combine the filtrate; concentrate the filtrate, The concentrated solution is obtained, and the ratio of the concentrated solution is 1:2-1:3, that is, each milliliter of the concentrated solution contains 0.5-0.33 grams of the original medicinal material;

[0032] (2) Enrichment of total saponins The concentrated solution obtained in step (1) is adsorbed through a macroporous adsorption resin, and the ratio of resin to the original medicinal material is 1: 0.1-10 g / g, that is, the amount of the original medicinal material adsorbed by every gram of resin 0.1 to 1 gram), respectively eluting with 20%-7...

Embodiment 1

[0038] Pearl ginseng raw material 1Kg, pulverized, 20 times the amount of water extraction 2 times, 2 hours each time, the extract was filtered, concentrated to 1:2, the filtrate was enriched with total saponins through a macroporous adsorption resin chromatographic column, and mixed with water and 60% ethanol Elute with aqueous solution, collect the ethanol eluate, concentrate under reduced pressure, and dry to obtain 110 g of total saponins; the total saponins are separated by a 5000 g silica gel chromatography column, eluted with a 20-5:1 chloroform-methanol gradient, and the eluate is collected. Concentrate under reduced pressure and dry to obtain 10 g of crude bamboo ginseng saponin IVa; the eluate is purified by Sephadex LH-20, eluted with chloroform-methanol gradient (20-5:1), and the eluate is collected , recovered under reduced pressure, dried to obtain the crude product of bamboo ginseng saponin IVa; the crude product was recrystallized repeatedly with acetone-methano...

Embodiment 2

[0040] Ginseng raw material 1Kg, pulverized, extracted twice with 15 times the amount of ethanol, each time for 1.5h, the extract was filtered, concentrated to 1:2, the filtrate was enriched through a macroporous adsorption resin chromatographic column, and mixed with water and 60% methanol aqueous solution Elution, collect methanol eluate, concentrate under reduced pressure, and dry to obtain 120 g of total saponins; 6000 g of total saponins are separated by silica gel chromatography, eluted with 20~5:1 chloroform-methanol gradient, collect eluate, and depressurize Concentrate and dry to obtain 7 g of crude product of bamboo ginseng saponin IVa; the eluate is purified by Sephadex LH-20, eluted with chloroform-methanol gradient (20-5:1), collects the eluate, and subtracts The crude product of bamboo ginseng saponin IVa was obtained by pressing and drying; the crude product was repeatedly recrystallized with acetone-ethanol to obtain 1.2 g of bamboo ginseng saponin IVa crystals,...

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Abstract

The invention discloses a method for preparing panax japonicus saponin IVa and an application of panax japonicus saponin IVa in preparing a medicament for protecting liver and lowering transaminase. The preparation method mainly comprises the following steps: crushing panax japonicus saponin IVa bulk pharmaceutical chemicals, extracting with water or a hydrophilic organic solvent, filtering and concentrating extract; passing the concentrated solution through a macroporous adsorption resin column chromatography column so as to enrich total saponin, eluting with water and an alcohol-water mixedliquid in turn, collecting the alcohol-water eluent, performing reduced pressure concentration at a low temperature, and drying to obtain the total saponin; and carrying out chromatograph separation on the total saponin, eluting with a solvent, recovering the solvent at reduced pressure, and drying so as to obtain crude panax japonicus saponin IVa; and repeatedly recrystallizing the crude productwith an organic solvent so as to obtain pure panax japonicus saponin IVa. According to verification, the panax japonicus saponin IVa has bioactivity of protecting liver and lowering transaminase, andcan be mixed with one or more of pharmaceutically acceptable carriers/auxiliary materials so as to be prepared into solid and liquid oral preparations with effects of protecting liver and lowering transaminase.

Description

technical field [0001] The present invention relates to a preparation method of a traditional Chinese medicine monomer and its medical use, in particular to a preparation method of an oleanolic acid type triterpene saponin-Pipetosaponin IVa in ginseng and its use in the preparation of liver-protecting and enzyme-lowering medicines Applications. Background technique [0002] Pearl ginseng is Araliaceae plant pearl ginseng [Panaxjaponicus C.A.Mey.var.major (Buck.) C.Y.Wu et K.M.Feng] or feather leaf Sanqi [Panax jap Onicus C.A.Mey.var.bipinnatifidus (Seem.) C.Y.Wu et K.M. Feng] dried beaded rhizomes. Mainly distributed in my country's Shaanxi, Sichuan, Hubei, Yunnan and other provinces. It has the effects of invigorating the lungs, nourishing yin, activating collaterals, and stopping bleeding. It is used for deficiency of both qi and yin, excessive heat and thirst, fatigue cough, injury from falls, joint pain, hemoptysis, hematemesis, traumatic bleeding, etc. Its aerial par...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00A61K31/704A61P1/16
Inventor 宋小妹崔九成蒙跃龙唐志书刘越宋蓓王薇冯改利邓翀晁旭
Owner SHAANXI UNIV OF CHINESE MEDICINE
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