Fluorescent quantitative PCR kit for detecting alpha-globin gene deletion

A fluorescent quantitative and globin technology, applied in the field of biochemistry, can solve the problems of operational feasibility, unsatisfactory detection throughput, false positives, false negatives, etc., and achieve good sensitivity, good specificity, repeatability, and good sensitivity and the effect of specificity
CN102146476AInactive Publication Date: 2011-08-10SOUTHERN MEDICAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTHERN MEDICAL UNIVERSITY
Publication Date
2011-08-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the field of biochemistry, and provides a fluorescent quantitative polymerase chain reaction (PCR) kit for detecting alpha-globin gene deletion. The kit consists of a pair of specific amplified xi globin primers, a pair of specific amplified alpha1 globin primers, a pair of specific amplified alpha2 globin primers, a pair of specific amplified beta-Actin gene primers, a fluorescent probe for specifically detecting xi globin, a fluorescent probe for specifically detecting alpha1 globin, a fluorescent probe for specifically detecting alpha2 globin, a fluorescent probe for specifically detecting beta-Actin genes, a DNA polymerase and the like. The kit has good sensitivity and accuracy for detecting depletion alpha-thalassemia, has good repeatability and stability, and is totally suitable for clinical detection of alpha-thalassemia.
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Description

technical field

[0001] The invention relates to the field of biochemistry, in particular to reagents for measuring or testing methods comprising nucleic acids. Background technique

[0002] The human α-globin gene cluster is located on chromosome 16. There are three functional genes ζ, α1, and α2, which express the corresponding ζ and α globin chains, and then combine with the corresponding β globin chains to form ζ2γ2 (embryonic hemoglobin Hb Portland) and α2β2 (adult hemoglobin HbA). Normally, the human globin gene expresses an appropriate ratio of α- and β-globin chains to form a functional hemoglobin tetramer. When the α-globin gene is defective, the α-chain synthesis is reduced and the β-chain is relatively excess, which can lead to α-thalassemia disease. Thalassemia (referred to as "thalassemia") is one of the most common single-gene genetic diseases that have the greatest impact on human health in the world. It is mainly concentrated in countries along the Mediterra...

Claims

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