Preparation method of anti-fatigue oligopeptides biscuits
An anti-fatigue and oligopeptide technology, applied in dough processing, baking, baked food, etc., can solve the problems of single nutrition and scarcity of functional biscuits, achieve obvious effects, increase nutritional value, and enhance antioxidant activity
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[0029] The preparation method of the anti-fatigue oligopeptide biscuit of the present invention comprises three parts: preparation of casein oligopeptide powder, preparation of wolfberry juice powder and preparation of anti-fatigue casein oligopeptide biscuit.
[0030] The preparation of casein oligopeptide powder is to hydrolyze cow milk or goat milk casein with neutral protease and alkaline protease for a specified time under the conditions of specified pH, temperature and enzyme amount, then inactivate the enzyme, cool, and centrifuge to remove the enzyme and unused Enzymatically hydrolyzed protein macromolecules are spray-dried.
[0031] The wolfberry juice powder is prepared by screening, cleaning, color-protecting, beating, leaching, press-filtering, fine-filtering, and spray-drying dry wolfberry that is free from diseases, insect pests, and mildew.
[0032] The preparation of anti-fatigue casein oligopeptide biscuits is to mix the prepared casein oligopeptide powder and...
Embodiment 1
[0043] (1) Take fresh goat milk, filter it to remove impurities, and centrifuge it at low temperature to degrease. Use 2 mol / L HCl solution to adjust the pH value to 4.3, stand still for 30 min, centrifuge at 3000 r / min for 10 min, discard the supernatant, wash the precipitate twice with acetic acid-sodium acetate buffer solution with pH 4.3, after washing Centrifuge at 3000 r / min for 10 minutes, and the obtained precipitate is crude casein;
[0044] Weigh a certain amount of crude casein and place it in an enzymolysis tank, dissolve it with 0.1 mol / L sodium hydroxide solution, and distill the volume to the specified volume with distilled water to obtain a 60 g / kg goat milk casein solution.
[0045] ( 2) Prepare a 60 g / kg goat milk casein solution, adjust the pH to 7.5, heat at 95°C for 5 minutes, cool to 50°C, add 3500 U / g of neutral protease and 250 U / g of alkaline protease and keep the temperature at 50°C For hydrolysis, add 2 mol / L NaOH solution during the hydrolysis pro...
Embodiment 2
[0049] (1) Fresh milk is filtered to remove impurities, and centrifuged at low temperature to degrease. Use 2 mol / L HCl solution to adjust the pH value to 4.6, stand still for 30 min, centrifuge at 3000 r / min for 10 min, discard the supernatant, wash the precipitate twice with acetic acid-sodium acetate buffer solution with pH 4.6, after washing Centrifuge at 3000 r / min for 10 min, and the obtained precipitate is crude casein;
[0050] Weigh a certain amount of crude casein and place it in an enzymolysis tank, dissolve it with 0.1 mol / L sodium hydroxide solution, and distill the volume to the specified volume with distilled water to obtain a 60 g / kg milk casein solution.
[0051] ( 2) Prepare a 60 g / kg milk casein solution, adjust the pH to 7.5, heat at 95°C for 5 minutes, cool to 50°C, add 4000 U / g neutral protease and 200 U / g alkaline protease to hydrolyze at constant temperature , During the hydrolysis process, 2 mol / L NaOH solution was added to keep it within the require...
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