Bone growth factor controlled release type bone repairing material as well as preparation method and applications thereof
A technology for bone growth factor and repair material, which is applied in the field of bone growth factor controlled-release bone repair material and its preparation, can solve the conditions that it is difficult to provide the synergistic effect of multiple growth factors, and the long-term slow-release effect of bone growth factor is not good. To achieve the effect of exerting osteoinductive effect, promoting bone tissue regeneration and functional reconstruction, and maintaining biological activity
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Embodiment 1
[0036] Embodiment 1 controlled-release BMP-2 / biological bone repair material
[0037] (1) Take by weighing 1.0g chitosan and be dissolved in the acetic acid solution of 0.5% (v / v), be made into the chitosan solution that concentration is mass volume ratio 0.1%, then under stirring (rotating speed 800r / min), press The mass ratio of bone morphogenetic protein-2 (BMP-2) to chitosan is 0.05: 1 ratio to add BMP-2, mix evenly;
[0038] (2) Weigh 1.0 g of sodium hyaluronate and dissolve it in deionized water to form a solution with a concentration of 0.1% by mass to volume;
[0039] (3) Weigh 0.5g of biological porous bone repair material (Guangdong Guanhao Biotechnology Co., Ltd. sample) and immerse it in the final solution made in step (1) under negative pressure (0.01MPa), keep it for 1 hour, take it out and dry it in the air ;
[0040] (4) Immerse the porous bone repair material treated in step (3) into the final solution prepared in step (2) under negative pressure (0.1 MPa), ...
Embodiment 2
[0042] Example 2 Controlled Release BMP-2 / bFGF / TCP Bone Repair Material
[0043] (1) Weigh 1.0g chitosan and dissolve it in 1.0% (v / v) acetic acid solution to make a solution with a concentration of 0.05% by mass volume ratio, and then, under stirring (rotating speed 3000r / min), press BMP-2 Add BMP-2 and bFGF in a ratio of 0.1:0.001:1 to the mass ratio of bFGF and chitosan, and mix well;
[0044] (2) Take by weighing 1.0g carboxymethyl chitosan and dissolve it in deionized water to make a solution with a concentration of 0.05% by mass to volume;
[0045] (3) Weigh 0.5g of calcium-phosphorus bioceramic porous bone repair material TCP (Wuhan Huawei Biomaterials Engineering Co., Ltd. β-TCP artificial bone, trade name: Bemet artificial bone) under negative pressure (0.1MPa) and immerse in step (1 ) in the solution made, keep it for 2 hours, take it out and dry it;
[0046] (4) Immerse the porous bone repair material processed in step (3) in the solution prepared in step (2) unde...
Embodiment 3
[0048] Embodiment 3: Controlled-release type BMP-2 / bFGF / biological bone repair material
[0049] (1) Weigh 1.0g of chitosan and dissolve it in 1.0% (v / v) acetic acid solution to make a solution with a concentration of 0.05% by mass volume ratio, and then, under stirring (rotating speed 2000r / min), press BMP-2 Add BMP-2 in a ratio of 0.1:1 to chitosan and mix evenly;
[0050] (2) Weigh 1.0 g of hyaluronic acid and dissolve it in deionized water to make a solution with a mass volume ratio of 0.05%, and then add bFGF at a ratio of 0.001:1 by mass ratio of bFGF to hyaluronic acid under stirring ,well mixed;
[0051] (3) Weigh 0.5 g of biological porous bone repair material and immerse it in the solution prepared in step (1) under negative pressure (0.1 MPa), keep it for 1 hour, take it out and dry it in the air;
[0052] (4) Immerse the porous bone repair material processed in step (3) in the solution prepared in step (2) under negative pressure (0.1 MPa), keep it for 1 hour, ta...
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