Method for preparing turnip chitin binding protein through macromolecular cold-condensation method

A technology of binding protein and coagulation method, which is applied in the field of preparing radish chitin binding protein by polymer condensation polymerization method, can solve the problems of complex preparation process, lack of relevant information, and difficulty in realizing industrialization, and achieves simple process, low equipment investment, The effect of easy industrial production
CN102174483AActive Publication Date: 2011-09-07ZHUHAI YUPINTANG BIOLOGICAL TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHUHAI YUPINTANG BIOLOGICAL TECH
Publication Date
2011-09-07

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Abstract

The invention relates to a method for preparing turnip chitin binding protein through a macromolecular cold-condensation method. The principles of PAA+R.CBP -> PAA-R.CBP and PAA-R.CBP+Ca<2+> -> PAA-Ca<2+>+R.CBP are utilized, turnip used as a material is purified to obtain R.CBP powder, wherein the purification step comprises induction, extraction, selective thermal denaturation, ultrafiltration concentration, condensation reaction and displacement. The technical production equipment has low investment, the technology is simple and easy to realize industrial production; and PAA can be recycled by adopting the reaction product PAA-Ca<2+> and using a H2SO4 solution, the cost can be reduced, and no environmental pollution is caused.
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Description

【Technical field】

[0001] The invention relates to a method for preparing radish chitin-binding protein by a polymer condensation condensation method. 【Background technique】

[0002] Radish chitin-binding protein (Raphanus sativus L Chitin-binding protin, abbreviated R·CBP, radish CBP) is a protein that can specifically bind to chitin, and it has been proved that it has a bactericidal effect (Journal of South China Agricultural University, 1997, 18(4):72~75): We have studied that this protein has a wide antibacterial spectrum, and it is effective against Staphylococcus aureus, Staphylococcus aureus, Bacillus Subtilis (Ehrenberg) Cohn Wait for G + bacteria and Proteus bacillus (Proteusbacillus Vulgaris), Escherichia Cdi, Salmonella typhimwium (Salmonellatyphimwium), Pastewella multaida (Pastewella multaida), Salmonella pullorum (Salmnella), Enterobacteratrogenes (Enterobacteratrogenes), etc. G - Fungi such as Saccharomyce Scerevisiae, Mucor racemosus, Rhizopus reserei, Aspe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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