Method for preparing turnip chitin binding protein through macromolecular cold-condensation method

A technology of binding protein and coagulation method, which is applied in the field of preparing radish chitin binding protein by polymer condensation polymerization method, can solve the problems of complex preparation process, lack of relevant information, and difficulty in realizing industrialization, and achieves simple process, low equipment investment, The effect of easy industrial production

Active Publication Date: 2011-09-07
ZHUHAI YUPINTANG BIOLOGICAL TECH
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Problems solved by technology

[0011] ⑦ The sequence of amino acid residues at the N-terminal is WDADMVAKUM. Blastp collected this sequence, and found that there is no relevant information matching it in the existing database
In medicine, there are very few antifungal drugs, and the application of antibiotics is limited due to the resistance of microorganisms and the side effects on the body; Pollution also limits the application. Also in food, daily chemical, and disease prevention and disease resistance industries in poultry and livestock breeding, radish CBP has a wide range of uses and needs to be developed and utilized.
[0013] Radishes can be planted all over the country, especially in Lingnan and other places all year round. The production and preparation of radish chitin-binding protein has a wide range of raw materials and is cheap, but the enzyme content is low. The existing preparation process is complicated and difficult to achieve. industrialization

Method used

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  • Method for preparing turnip chitin binding protein through macromolecular cold-condensation method
  • Method for preparing turnip chitin binding protein through macromolecular cold-condensation method
  • Method for preparing turnip chitin binding protein through macromolecular cold-condensation method

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Embodiment Construction

[0037] like figure 1 As shown, get the radish and wash it, cut its epidermis (1-2cm) with a knife, and induce it for 5 days at 16-20°C. Get 100kg of the induced radish, chop it, and add 100L 5mmol / L while homogenizing it. NaHCO 3 The solution was homogenized to obtain the first homogenate, and the first homogenate was centrifuged with a tubular centrifuge (14000r / min, room temperature) to obtain about 175L of the first supernatant; the residue was added with 100L5mmol / L NaHCO 3Homogenize the solution to obtain the second homogenate, and the second homogenate is centrifuged (same as above) to obtain 105L of the second supernatant; combine the first supernatant and the second supernatant (about 280L), and dry the residue (dried ) is used as feed; the above combined supernatant (pH about 4.5-5.0) is transferred to a reaction tank and stirred and heated to 50°C, kept for 5min, centrifuged (same as above), and the third supernatant is collected; the third supernatant The clear li...

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Abstract

The invention relates to a method for preparing turnip chitin binding protein through a macromolecular cold-condensation method. The principles of PAA+R.CBP -> PAA-R.CBP and PAA-R.CBP+Ca<2+> -> PAA-Ca<2+>+R.CBP are utilized, turnip used as a material is purified to obtain R.CBP powder, wherein the purification step comprises induction, extraction, selective thermal denaturation, ultrafiltration concentration, condensation reaction and displacement. The technical production equipment has low investment, the technology is simple and easy to realize industrial production; and PAA can be recycled by adopting the reaction product PAA-Ca<2+> and using a H2SO4 solution, the cost can be reduced, and no environmental pollution is caused.

Description

【Technical field】 [0001] The invention relates to a method for preparing radish chitin-binding protein by a polymer condensation condensation method. 【Background technique】 [0002] Radish chitin-binding protein (Raphanus sativus L Chitin-binding protin, abbreviated R·CBP, radish CBP) is a protein that can specifically bind to chitin, and it has been proved that it has a bactericidal effect (Journal of South China Agricultural University, 1997, 18(4):72~75): We have studied that this protein has a wide antibacterial spectrum, and it is effective against Staphylococcus aureus, Staphylococcus aureus, Bacillus Subtilis (Ehrenberg) Cohn Wait for G + bacteria and Proteus bacillus (Proteusbacillus Vulgaris), Escherichia Cdi, Salmonella typhimwium (Salmonellatyphimwium), Pastewella multaida (Pastewella multaida), Salmonella pullorum (Salmnella), Enterobacteratrogenes (Enterobacteratrogenes), etc. G - Fungi such as Saccharomyce Scerevisiae, Mucor racemosus, Rhizopus reserei, Aspe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/00
Inventor 苏保荣徐凤彩程京燕胡永刚
Owner ZHUHAI YUPINTANG BIOLOGICAL TECH
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